Showing papers on "Gelatin published in 2018"
TL;DR: A framework for evaluating printability was developed and the effect of dynamic modulus, including storage modulus (G'), loss modulus(G″), and loss tangent (G″/G') on the printing outcome was investigated to evaluate the printability of hydrogel-based bioinks.
Abstract: Three-dimensional bioprinting has emerged as a promising technique in tissue engineering applications through the precise deposition of cells and biomaterials in a layer-by-layer fashion. However, the limited availability of hydrogel bioinks is frequently cited as a major issue for the advancement of cell-based extrusion bioprinting technologies. It is well known that highly viscous materials maintain their structure better, but also have decreased cell viability due to the higher forces which are required for extrusion. However, little is known about the effect of the two distinct components of dynamic modulus of viscoelastic materials, storage modulus (G') and loss modulus (G″), on the printability of hydrogel-based bioinks. Additionally, 'printability' has been poorly defined in the literature, mostly consisting of gross qualitative measures which do not allow for direct comparison of bioinks. This study developed a framework for evaluating printability and investigated the effect of dynamic modulus, including storage modulus (G'), loss modulus (G″), and loss tangent (G″/G') on the printing outcome. Gelatin and alginate as model hydrogels were mixed at various concentrations to obtain hydrogel formulations with a wide range of storage and loss moduli. These formulations were then evaluated for the quantitatively defined values of extrudability, extrusion uniformity, and structural integrity. For extrudability, increasing either the loss or storage modulus increased the pressure required to extrude the bioink. A mathematical model relating the G' and G″ to the required extrusion pressure was derived based on the data. A lower loss tangent was correlated with increased structural integrity while a higher loss tangent correlated with increased extrusion uniformity. Gelatin-alginate composite hydrogels with a loss tangent in the range of 0.25-0.45 exhibited an excellent compromise between structural integrity and extrusion uniformity. In addition to the characterization of a common bioink, the methodology introduced in this paper could also be used to evaluate the printability of other bioinks in the future.
310 citations
TL;DR: This review describes the new trends in nanomaterials based drug delivery systems mainly using biopolymers such as proteins (silk fibroin, collagen, gelatin and albumin) and polysaccharides (chitosan, alginate, cellulose and starch).
303 citations
TL;DR: A gelatin‐based, strong, ductilehydrogel is prepared by soaking a virgin gelatin hydrogel in ammonium sulfate solutions to endow the hydrogels with superior mechanical properties.
Abstract: A gelatin‐based, strong, ductile hydrogel is prepared by soaking a virgin gelatin hydrogel in ammonium sulfate solutions. Chain bundling, hydrophobic interactions, and microphase separation regions induced by the Hofmeister effect endow the hydrogels with superior mechanical properties.
231 citations
TL;DR: In this article, the authors have successfully fabricated hybrid nanocomposite film consisting of chitosan, gelatin, polyethylene glycol and silver nanoparticles (AgNPs) by solution casting method.
216 citations
TL;DR: Incorporating TP into gelatin and sodium alginate film solution was an effective method in order to improve physical properties and antioxidant activity of the films.
208 citations
TL;DR: In this paper, a fully biodegradable triboelectric nanogenerator (BD-TENG) based on gelatin film and electrospun polylactic acid nanofiber membrane is presented.
196 citations
TL;DR: The tannin-inspired gelatin bioadhesives hold great promise for a number of applications in wound closure, tissue sealant, hemostasis, antimicrobial and cell/drug delivery, and would be interested to the readers from biomaterials, tissue engineering, and drug delivery area.
184 citations
TL;DR: In this paper, mango peels extract (MPE) was incorporated into fish gelatin films to determine their physical, barrier, mechanical and antioxidant properties for active food packaging, which revealed the benefits of mango by-products incorporated into gelatin based films as a potential material for active packaging.
180 citations
TL;DR: In this article, a comparative study of gelatin sponges was conducted to identify a biocompatible and effective crosslinker for preparing gelatin spondylus, including Glutaraldehyde (GTA), genipin (GP), 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide (EDC), and microbial transglutaminase (mTG) was used as crosslinkers.
Abstract: This comparative study aims to identify a biocompatible and effective crosslinker for preparing gelatin sponges. Glutaraldehyde (GTA), genipin (GP), 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide (EDC), and microbial transglutaminase (mTG) were used as crosslinking agents. The physical properties of the prepared samples were characterized, and material degradation was studied in vitro with various proteases and in vivo through subcutaneous implantation of the sponges in rats. Adipose-derived stromal stem cells (ADSCs) were cultured and inoculated onto the scaffolds to compare the cellular biocompatibility of the sponges. Cellular seeding efficiency and digestion time of the sponges were also evaluated. Cellular viability and proliferation in scaffolds were analyzed by fluorescence staining and MTT assay. All the samples exhibited high porosity, good swelling ratio, and hydrolysis properties; however, material strength, hydrolysis, and enzymolytic properties varied among the samples. GTA-sponge and GP-sponge possessed high compressive moduli, and EDC-sponge exhibited fast degradation performance. GTA and GP sponge implants exerted strong in vivo rejections, and the former showed poor cell growth. mTG-sponge exhibited the optimal comprehensive performance, with good porosity, compressive modulus, anti-degradation ability, and good biocompatibility. Hence, mTG-sponge can be used as a scaffold material for tissue engineering applications.
171 citations
TL;DR: Sensory evaluation results implied that the gelatin/CO@CNPs nanofibers treatment could maintain the color and flavor of cucumber well for >4days.
163 citations
TL;DR: Results show that increasing nano-cellulose composition to 10% leads to increase the tensile strength at break to 8121 MN/m2 and decrease the elongation at break and increasing chitosan composition from 5% to 30% can enhance food preservation up to 15 days.
TL;DR: The syneresis of starch gel during freeze-thaw process was reduced by the addition of pectin, and a significant increase was observed in both the proportion of slowly digestible starch and resistant starch for retrograded starch-pectin mixtures.
01 May 2018
TL;DR: A facile approach without chemical modification is presented to construct injectable gelatin-based hydrogels, which have great potential as a wound dressing or tissue scaffold at body temperature.
Abstract: Injectable scaffolds are of great interests for skin regeneration because they can fill irregularly shaped defects through minimally invasive surgical treatments. In this study, an injectable hydrogel from biopolymers is developed and its application as wound dressings is examined. Gelatin-based hydrogels were successfully prepared at body temperature upon blending with low content of gellan, and the synergetic effect on the gel formation was carefully characterized through rheological methods. The electrostatic complexation between gelatin and gellan was confirmed to contribute a continuous hydrogel network. The obtained blend hydrogel demonstrates remarkable shear-thinning and self-recovering properties. For antibacterial purpose, tannic acid was incorporated into the blend hydrogel. In addition, tannic acid-loaded blend hydrogel was verified to accelerate the wound healing on the mice model, significantly than the control groups. Thus, this paper presents a facile approach without chemical modification...
TL;DR: The potential usefulness of Gel-CDH/HA-mCHO hydrogel for tissue-engineering scaffolds is demonstrated, which consisted of carbohydrazide-modified gelatin and hyaluronic acid monoaldehyde, which were sufficiently stable during the angiogenesis process.
TL;DR: The selected optimal microcapsules revealed intense red color over the time of storage, implying the effectiveness of the method chosen to preserve anthocyanins, particularly under harsh processing and storage conditions.
TL;DR: In this article, transparent and colorless citric acid-incorporated fish gelatin/chitosan composite films were prepared in order to demonstrate the potential use of these films as active food packaging.
TL;DR: In this article, eugenol and ginger essential oils were incorporated in different film formulations to produce active films that might be used as food packaging, and optical, microstructural, mechanical, and barrier properties were characterized.
Abstract: Eugenol and ginger essential oils were incorporated in different film formulations to produce active films that might be used as food packaging. Optical, microstructural, mechanical, and barrier properties were characterized, as well as their antioxidant activity. Fourier transformed infrared spectroscopy analysis confirmed the presence of new bands with addition of eugenol or ginger essential oils, and scanning electron microscopy and atomic force microscopy analyses showed an increases in roughness values (p
TL;DR: In this article, the gelatin, zein, and gelatin/zein films were fabricated by electrospinning and solvent casting, respectively, and the results showed that the zein particles prevented the gelatin chains from aggregating intramolecularly in the casted films due to heterogeneous mixing, while strong hydrogen bonding was formed in the nanofibrous film as a result of homogeneous mixing.
TL;DR: In this paper, the gelatin/zein nanofibers were fabricated by hybrid electrospinning and the results of Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that gelatin and zein strongly interacted via hydrogen bonding.
TL;DR: In this paper, the authors focused on the production of biodegradable food packaging from renewable materials, primarily gelatin, and the effect of the biomaterials used on functional properties of the films produced needs thorough investigation.
Abstract: Food packaging derived from a petroleum base represents a serious environmental problem. Finding alternative sustainable solutions is a must. Therefore, the current study has focused on the production of biodegradable food packaging from renewable materials, primarily gelatin. The effect of the biomaterials used on functional properties of the films produced needs thorough investigation. Gelatin represents interesting biomaterials for developing biodegradable food packaging, mainly due to their good film forming properties and abundantly in nature. However, the incorporation of gelatin in biodegradable films for food packaging may give some drawback on certain properties of the film such as tensile strength and water vapour permeability. Thus, addition of plasticizers into the film materials improves the functional properties of films by increasing their extensibility, dispensability, flexibility, elasticity, and rigidity. This study aims to review the current findings on how plasticizers impact the functional properties of biodegradable gelatin-based films. Plasticizers incorporation in the films may affect the continuity of the polymer matrix, leading to physical changes, where the films become more flexible and stretchable. Generally, the plasticization effect of plasticizers strengthens the film structure, in which the tensile strength and elongation of the films are improved and water barrier properties are reduced.
TL;DR: Focus on alginate/gelatin scaffolds with uniform nano apatite coating, which had 2-fold higher Young's modulus and significantly stimulated the proliferation and osteogenic differentiation of rat bone marrow stem cells.
TL;DR: A method to 3D print gelatin into well-defined repetitive geometries that show clear differences in biological effects on seeded hepatocytes are described, and it is shown that a relatively simple and widely used biomaterial, such as gelatin, can significantly modulate biological processes when fabricated into specific 3D geometry.
TL;DR: Along with their injectability and tunable mechanical properties, the photocurable thiol-acrylate hydrogels showed promise as corneal substitutes or substrates to construct a new cornean tissue.
Abstract: In this study, an injectable, photocurable gelatin system, consisting of acrylated gelatin and thiolated gelatin, with tunable mechanical, biodegradation, and biological properties was used as a potential cell-supportive scaffold for the repair of focal corneal wounds. The mechanical property of hydrogels can be readily modified (postcure shear modulus of between 0.3 and 22 kPa) by varying the ratio of acrylate to thiol groups, photointensity, and solid content, and the biodegradation times also varied with the change of solid content. More importantly, the generated hydrogels exhibited excellent cell viability in both cell seeding and cell encapsulation experiments. Furthermore, the hydrogels were found to be biocompatible with rabbit cornea and aided the regeneration of a new tissue under a focal corneal wound (exhibiting epithelial wound coverage in <3d), and ultraviolet irradiation did not have any obvious harmful effect on the cornea and posterior eye segment tissues. Along with their injectability and tunable mechanical properties, the photocurable thiol-acrylate hydrogels showed promise as corneal substitutes or substrates to construct a new corneal tissue.
TL;DR: It is suggested that the aldehyde end groups in alginate chains have the dominant effect in crosslinking with gelatin.
TL;DR: An edible and pH-sensitive film combined with electrochemical writing was developed by using gelatin, gellan gum, and red radish anthocyanins extract for intelligent food packaging, which showed an orange red-to-yellow color change in the pH range of 2-12.
Abstract: An edible and pH-sensitive film combined with electrochemical writing was developed by using gelatin, gellan gum, and red radish anthocyanins extract for intelligent food packaging. The composite f...
TL;DR: Observations depicted that chitosan/gelatin hydrogel film can be an ideal delivery system for sustained released of lupeol and LCGH film for enhanced wound healing.
TL;DR: Results showed that addition of GT to SF increased nanofiber's diameter, bulk hydrophilicity, surface wettability, mass loss percentage, but decreased Young's modulus, tensile strength, and porosity of the SF/GT mats.
Abstract: In this study, a nanofibrous electrospun substrate based on the silk fibroin (SF) and gelatin (GT) polymers were prepared and evaluated. The SF/GT blended solutions were prepared with various ratios of GT in formic acid and electrospun to obtain bead-free fibers. Results showed that addition of GT to SF increased nanofiber's diameter, bulk hydrophilicity, surface wettability, mass loss percentage, but decreased Young's modulus, tensile strength, and porosity of the SF/GT mats. According to the obtained results, the mat containing 10% of GT was selected as the optimized mat for further studies and loaded with thyme essential oil (TEO) and doxycycline monohydrate (DCMH) as the antibacterial agents. Release studies showed a burst release of TEO from the mat within the first 3 h, while the DCMH had a sustained release during 48 h. In comparison to the TEO-loaded mat, the DCMH-loaded one showed larger inhibition zones against Staphylococcus aureus and Klebsiella pneumoniae bacteria. Meanwhile, cellular studies using mouse fibroblast L929 cells showed excellent cell-compatibility of TEO- and DCMH-loaded mats. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1092-1103, 2018.
TL;DR: In this article, the gelatin nanofibers containing thyme essential oil/β-cyclodextrin e-polylysine nanoparticles (TCPNs) were engineered.
Abstract: Campylobacter jejuni (C. jejuni) contamination on poultry surface posed great threats to meat industry and human health. In order to control the propagation of C. jejuni, the gelatin nanofibers containing thyme essential oil/β-cyclodextrin e-polylysine nanoparticles (TCPNs) were engineered. Firstly, TCPNs were successfully fabricated via ionic gelation. The prepared TCPNs showed excellent antimicrobial activity against C. jejuni, which caused membranolysis and protein leakage of C. jejuni. Subsequently, TCPNs were incorporated into gelatin nanofibrous matrix via electrospinning to produce antimicrobial nanofibers. Uniformly disordered fibrous structure with good continuity and fine diameter distribution was observed. Finally, the aerobic bacterial count, thiobarbituric acid, total volatile basic nitrogen, pH, color and texture of meat samples packaged with the nanofibers were detected. The results implied the TCPNs embedded gelatin nanofibers had a promising prospect in meat preservation without impact on sensory evaluation.
TL;DR: It was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA, and the increase of either GelMA or CS concentration can strengthen the hydrogel network.
TL;DR: In this paper, the effect of crosslinker volume and gelatin weight percent on the adsorption performance of cellulose hydrogels was investigated, and the results showed that gelatin can significantly improve the swelling ability and the adorption capacity.
Abstract: Hydrogel is a promising adsorbent for heavy metal removal. In order to conserve the environment by avoiding the hydrogel disposal problem after the adsorption process, this research work was carried out to explore the feasibility in synthesizing biodegradable cellulose hydrogel and cellulose/gelatin composite hydrogel. The effect of cross-linker volume percent and gelatin weight percent towards the adsorption capacity of hydrogels were investigated. Cellulose hydrogel with the lowest cross-linker volume percent, H6 (6 v/v%) has the greatest swelling ability (4650%), highest biodegradability rate (79.5%), and highest adsorption capacity (28.4 mg/g) compared to other cellulose hydrogels. Less compact structure of H6 has the potential to facilitate the movement of Cu2+ ions into the hydrogel network, increased the accessibility of the Cu2+ ions onto the active sites of the hydrogel, and thus enhanced its characteristics and adsorption performance. The addition of gelatin into cellulose hydrogel network has further improved the swelling ability and adsorption capacity of cellulose/gelatin composite hydrogel attributed to the presence of amine group from gelatin which has stronger affinity towards water absorption and presented stronger binding propensity towards Cu2+ ions. The experiment data was fitted better to the Freundlich model, indicating the occurrence of multilayer adsorption. This success of this research study has confirmed the potential application of cellulose hydrogel and cellulose/gelatin composite hydrogel as a more sustainable and environmental-friendly adsorbent for the removal of heavy metal from wastewater.