Klaus Giewekemeyer

TL;DR: It is shown that the projected electron density of unstained and unsliced freeze-dried cells of the bacterium Deinococcus radiodurans can be derived from the reconstructed phase in a straightforward and reproducible way, with quantified and small errors.

TL;DR: In this article, the authors carried out a ptychographic scanning coherent diffraction imaging experiment on a test object in order to characterize the hard x-ray nanobeam in a scanning X-ray microscope and obtained a detailed quantitative picture of the complex wave field in the nanofocus with high spatial resolution and dynamic range.

TL;DR: It is demonstrated that high-quality and damage-free protein structures can be obtained with the currently available 1.1 MHz repetition rate pulses using lysozyme as a test case and furthermore a β-lactamase structure.

TL;DR: 3D hard X-ray imaging of unstained bacterial cells by a combination of ptychography and tomography is presented and it is shown how the real and reciprocal space approach can be used synergistically on the same sample and with the same setup.

TL;DR: To highlight a biological system where resonant ptychography might be used for chemical mapping of unsliced cells, reconstructions of freeze-dried Deinococcus radiodurans cells at an energy of 517 eV are shown.