C
Christopher Kupitz
Researcher at University of Wisconsin–Milwaukee
Publications - 59
Citations - 5353
Christopher Kupitz is an academic researcher from University of Wisconsin–Milwaukee. The author has contributed to research in topics: Femtosecond & Laser. The author has an hindex of 24, co-authored 51 publications receiving 4616 citations. Previous affiliations of Christopher Kupitz include Arizona's Public Universities & Arizona State University.
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Journal ArticleDOI
High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography
Sébastien Boutet,Lukas Lomb,Garth J. Williams,Thomas R. M. Barends,Andrew Aquila,R. Bruce Doak,Uwe Weierstall,Daniel P. DePonte,Jan Steinbrener,Robert L. Shoeman,Marc Messerschmidt,Anton Barty,Thomas A. White,Stephan Kassemeyer,Richard A. Kirian,M. Marvin Seibert,Paul A. Montanez,C.J. Kenney,Ryan Herbst,P. Hart,J. Pines,Gunther Haller,Sol M. Gruner,Hugh T. Philipp,Mark W. Tate,Marianne Hromalik,Lucas J. Koerner,Niels van Bakel,John Morse,W. Ghonsalves,David Arnlund,Michael J. Bogan,Carl Caleman,Raimund Fromme,Christina Y. Hampton,Mark S. Hunter,Linda C. Johansson,Gergely Katona,Christopher Kupitz,Mengning Liang,Andrew V. Martin,Karol Nass,Lars Redecke,Lars Redecke,Francesco Stellato,Nicusor Timneanu,Dingjie Wang,Nadia A. Zatsepin,Donald W. Schafer,James Defever,Richard Neutze,Petra Fromme,John C. H. Spence,Henry N. Chapman,Ilme Schlichting +54 more
TL;DR: Serial femtosecond crystallography (SFX) is applied using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals of the well-characterized model protein lysozyme, demonstrating the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.
Journal ArticleDOI
Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser
Yanyong Kang,X. Edward Zhou,Xiang Gao,Yuanzheng He,Wei Liu,Andrii Ishchenko,Anton Barty,Thomas A. White,Oleksandr Yefanov,Gye Won Han,Qingping Xu,Parker W. de Waal,Jiyuan Ke,M. H. Eileen Tan,Chenghai Zhang,Arne Moeller,Graham M. West,Bruce D. Pascal,Ned Van Eps,Lydia N. Caro,Sergey A. Vishnivetskiy,Regina J. Lee,Kelly Suino-Powell,Xin Gu,Kuntal Pal,Jinming Ma,Xiaoyong Zhi,Sébastien Boutet,Garth J. Williams,Marc Messerschmidt,Cornelius Gati,Nadia A. Zatsepin,Dingjie Wang,Daniel James,Shibom Basu,Shatabdi Roy-Chowdhury,Chelsie E. Conrad,Jesse Coe,Haiguang Liu,Stella Lisova,Christopher Kupitz,Ingo Grotjohann,Raimund Fromme,Yi Jiang,Minjia Tan,Huaiyu Yang,Jun Li,Meitian Wang,Zhong Zheng,Dianfan Li,Nicole Howe,Yingming Zhao,Jörg Standfuss,Kay Diederichs,Yuhui Dong,Clinton S. Potter,Bridget Carragher,Martin Caffrey,Hualiang Jiang,Henry N. Chapman,John C. H. Spence,Petra Fromme,Uwe Weierstall,Oliver P. Ernst,Vsevolod Katritch,Vsevolod V. Gurevich,Patrick R. Griffin,Wayne L. Hubbell,Raymond C. Stevens,Vadim Cherezov,Karsten Melcher,H. Eric Xu +71 more
TL;DR: The crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin is determined by serial femtosecond X-ray laser crystallography and provides a basis for understanding GPCR-mediated arrestin-biased signalling.
Journal ArticleDOI
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
Uwe Weierstall,Daniel James,Chong Wang,Thomas A. White,Dingjie Wang,Wei Liu,John C. H. Spence,R. Bruce Doak,Garrett Nelson,Petra Fromme,Raimund Fromme,Ingo Grotjohann,Christopher Kupitz,Nadia A. Zatsepin,Haiguang Liu,Shibom Basu,Daniel Wacker,Gye Won Han,Vsevolod Katritch,Sébastien Boutet,Marc Messerschmidt,Garth J. Williams,Jason E. Koglin,M. Marvin Seibert,M. Marvin Seibert,Markus Klinker,Cornelius Gati,Robert L. Shoeman,Anton Barty,Henry N. Chapman,Richard A. Kirian,Kenneth R. Beyerlein,Raymond C. Stevens,Dianfan Li,Syed T. A. Shah,Nicole Howe,Martin Caffrey,Vadim Cherezov +37 more
TL;DR: This work presents a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography.
Journal ArticleDOI
Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser.
Christopher Kupitz,Shibom Basu,Ingo Grotjohann,Raimund Fromme,Nadia A. Zatsepin,Kimberly N. Rendek,Mark S. Hunter,Mark S. Hunter,Robert L. Shoeman,Thomas A. White,Dingjie Wang,Daniel James,Jay-How Yang,Danielle E. Cobb,Brenda Reeder,Raymond G. Sierra,Haiguang Liu,Anton Barty,Andrew Aquila,Daniel P. DePonte,Richard A. Kirian,Sadia Bari,Jesse J. Bergkamp,Kenneth R. Beyerlein,Michael J. Bogan,Carl Caleman,Tzu-Chiao Chao,Tzu-Chiao Chao,Chelsie E. Conrad,Katherine M. Davis,Holger Fleckenstein,Lorenzo Galli,Stefan P. Hau-Riege,Stephan Kassemeyer,Hartawan Laksmono,Mengning Liang,Lukas Lomb,Stefano Marchesini,Andrew V. Martin,Marc Messerschmidt,Despina Milathianaki,Karol Nass,Alexandra Ros,Shatabdi Roy-Chowdhury,Kevin Schmidt,M. Marvin Seibert,M. Marvin Seibert,Jan Steinbrener,Francesco Stellato,Lifen Yan,Chun Hong Yoon,Thomas A. Moore,Ana L. Moore,Yulia Pushkar,Garth J. Williams,Sébastien Boutet,R. Bruce Doak,Uwe Weierstall,Matthias Frank,Henry N. Chapman,John C. H. Spence,Petra Fromme +61 more
TL;DR: Time resolved experiments on PSII nano/microcrystals from Thermosynechococcus elongatus performed with the recently developed technique of serial femtosecond crystallography provide evidence that PSII undergoes significant conformational changes at the electron acceptor side and at the Mn4CaO5 core of the OEC.
Journal ArticleDOI
Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein
Jason Tenboer,Shibom Basu,Nadia A. Zatsepin,Kanupriya Pande,Despina Milathianaki,Matthias Frank,Mark S. Hunter,Sébastien Boutet,Garth J. Williams,Jason E. Koglin,Dominik Oberthuer,Michael Heymann,Christopher Kupitz,Chelsie E. Conrad,Jesse Coe,Shatabdi Roy-Chowdhury,Uwe Weierstall,Daniel James,Dingjie Wang,Thomas D. Grant,Anton Barty,Oleksandr Yefanov,Jennifer Scales,Cornelius Gati,Carolin Seuring,Vukica Šrajer,Robert Henning,Peter Schwander,Raimund Fromme,Abbas Ourmazd,Keith Moffat,Jasper J. van Thor,John C. H. Spence,Petra Fromme,Henry N. Chapman,Marius Schmidt +35 more
TL;DR: This work used microcrystals of photoactive yellow protein as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features, which open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.