Showing papers by "Fraunhofer Society published in 2005"

A critical review of interfaces with the peripheral nervous system for the control of neuroprostheses and hybrid bionic systems

Abstract: Considerable scientific and technological efforts have been devoted to develop neuroprostheses and hybrid bionic systems that link the human nervous system with electronic or robotic prostheses, with the main aim of restoring motor and sensory functions in disabled patients. A number of neuroprostheses use interfaces with peripheral nerves or muscles for neuromuscular stimulation and signal recording. Herein, we provide a critical overview of the peripheral interfaces available and trace their use from research to clinical application in controlling artificial and robotic prostheses. The first section reviews the different types of non-invasive and invasive electrodes, which include surface and muscular electrodes that can record EMG signals from and stimulate the underlying or implanted muscles. Extraneural electrodes, such as cuff and epineurial electrodes, provide simultaneous interface with many axons in the nerve, whereas intrafascicular, penetrating, and regenerative electrodes may contact small groups of axons within a nerve fascicle. Biological, technological, and material science issues are also reviewed relative to the problems of electrode design and tissue injury. The last section reviews different strate- gies for the use of information recorded from peripheral interfaces and the current state of control neuroprostheses and hybrid bionic systems.

Collision Detection for Deformable Objects

Abstract: Interactive environments for dynamically deforming objects play an important role in surgery simulation and entertainment technology. These environments require fast deformable models and very efficient collision handling techniques. While collision detection for rigid bodies is well investigated, collision detection for deformable objects introduces additional challenging problems. This paper focuses on these aspects and summarizes recent research in the area of deformable collision detection. Various approaches based on bounding volume hierarchies, distance fields and spatial partitioning are discussed. In addition, image-space techniques and stochastic methods are considered. Applications in cloth modeling and surgical simulation are presented.

Spatio-spectral filters for improving the classification of single trial EEG

Abstract: Data recorded in electroencephalogram (EEG)-based brain-computer interface experiments is generally very noisy, nonstationary, and contaminated with artifacts that can deteriorate discrimination/classification methods. In this paper, we extend the common spatial pattern (CSP) algorithm with the aim to alleviate these adverse effects. In particular, we suggest an extension of CSP to the state space, which utilizes the method of time delay embedding. As we will show, this allows for individually tuned frequency filters at each electrode position and, thus, yields an improved and more robust machine learning procedure. The advantages of the proposed method over the original CSP method are verified in terms of an improved information transfer rate (bits per trial) on a set of EEG-recordings from experiments of imagined limb movements.

Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo

Abstract: In optoacoustic imaging, short laser pulses irradiate highly scattering human tissue and adiabatically heat embedded absorbing structures, such as blood vessels, to generate ultrasound transients by means of the thermoelastic effect. We present an optoacoustic vascular imaging system that records these transients on the skin surface with an ultrasound transducer array and displays the images online. With a single laser pulse a complete optoacoustic B-mode image can be acquired. The optoacoustic system exploits the high intrinsic optical contrast of blood and provides high-contrast images without the need for contrast agents. The high spatial resolution of the system is determined by the acoustic propagation and is limited to the submillimeter range by our 7.5-MHz linear array transducer. A Q-switched alexandrite laser emitting short near-infrared laser pulses at a wavelength of 760 nm allows an imaging depth of a few centimeters. The system provides real-time images at frame-rates of 7.5 Hz and optionally displays the classically generated ultrasound image alongside the optoacoustic image. The functionality of the system was demonstrated in vivo on human finger, arm and leg. The proposed system combines the merits and most compelling features of optics and ultrasound in a single high-contrast vascular imaging device.

ProMiner: rule-based protein and gene entity recognition

Abstract: Identification of gene and protein names in biomedical text is a challenging task as the corresponding nomenclature has evolved over time. This has led to multiple synonyms for individual genes and proteins, as well as names that may be ambiguous with other gene names or with general English words. The Gene List Task of the BioCreAtIvE challenge evaluation enables comparison of systems addressing the problem of protein and gene name identification on common benchmark data. The ProMiner system uses a pre-processed synonym dictionary to identify potential name occurrences in the biomedical text and associate protein and gene database identifiers with the detected matches. It follows a rule-based approach and its search algorithm is geared towards recognition of multi-word names [1]. To account for the large number of ambiguous synonyms in the considered organisms, the system has been extended to use specific variants of the detection procedure for highly ambiguous and case-sensitive synonyms. Based on all detected synonyms for one abstract, the most plausible database identifiers are associated with the text. Organism specificity is addressed by a simple procedure based on additionally detected organism names in an abstract. The extended ProMiner system has been applied to the test cases of the BioCreAtIvE competition with highly encouraging results. In blind predictions, the system achieved an F-measure of approximately 0.8 for the organisms mouse and fly and about 0.9 for the organism yeast.

Ceramic Diesel Particulate Filters

Abstract: Twenty-five years of diesel particulate filter (DPF) developments have shown that ceramic materials are well-suited candidates to fulfill the harsh requirements of exhaust after treatment. The introduction of DPF in passenger cars in Europe in 2000 was a real breakthrough from both a scientific and a commercial point of view. Different systems and filter materials can be used as DPF; however, at the moment silicon carbide wall flow filters seem to be at advantage. There is a continual demand for cost-effective and reliable materials and systems forced by increasing legal emission standards.

Combining Atom Transfer Radical Polymerization and Click Chemistry: A Versatile Method for the Preparation of End-Functional Polymers

Abstract: Summary: The bromine chain ends of well-defined polystyrene ( = 2 700 g · mol−1, = 1.11) prepared using ATRP were successfully transformed into various functional end groups (ω-hydroxy, ω-carboxyl and ω-methyl-vinyl) by a two-step pathway: (1) substitution of the bromine terminal atom by an azide function and (2) 1,3-dipolar cycloaddition of the terminal azide and functional alkynes (propargyl alcohol, propiolic acid and 2-methyl-1-buten-3-yne). The “click” cycloaddition was catalyzed efficiently by the system copper bromide/4,4′-di-(5-nonyl)-2,2′-bipyridine. In all cases, 1H NMR spectra indicated quantitative transformation of the chain ends of polystyrene into the desired function. Preparation of well-defined functional polymers possessing diverse chain-end functionalities by the combination of atom transfer radical polymerization and click chemistry.

The ultrasmoothness of diamond-like carbon surfaces.

Abstract: The ultrasmoothness of diamond-like carbon coatings is explained by an atomistic/continuum multiscale model. At the atomic scale, carbon ion impacts induce downhill currents in the top layer of a growing film. At the continuum scale, these currents cause a rapid smoothing of initially rough substrates by erosion of hills into neighboring hollows. The predicted surface evolution is in excellent agreement with atomic force microscopy measurements. This mechanism is general, as shown by similar simulations for amorphous silicon. It explains the recently reported smoothing of multilayers and amorphous transition metal oxide films and underlines the general importance of impact-induced downhill currents for ion deposition, polishing, and nanopattering.

Molecular farming for new drugs and vaccines. Current perspectives on the production of pharmaceuticals in transgenic plants

Abstract: The European Union Framework 6 Pharma–Planta Consortium The first recombinant plant‐derived pharmaceutical protein (PDP) was human serum albumin, initially produced in 1990 in transgenic tobacco and potato plants (Sijmons et al , 1990). Fifteen years on, the first technical proteins produced in transgenic plants are on the market, and proof of concept has been established for the production of many therapeutic proteins, including antibodies, blood products, cytokines, growth factors, hormones, recombinant enzymes and human and veterinary vaccines (Twyman et al , 2005). Furthermore, several PDP products for the treatment of human diseases are approaching commercialization (Table 1), including recombinant gastric lipase for the treatment of cystic fibrosis, and antibodies for the prevention of dental caries and the treatment of non‐Hodgkin's lymphoma (Ma et al , 2003). There are also several veterinary vaccines in the pipeline; Dow AgroSciences (Indianapolis, IN, USA) announced recently their intention to produce plant‐based vaccines for the animal health industry. View this table: Table 1. Plant‐derived pharmaceutical proteins that are closest to commercialization for the treatment of human diseases As molecular farming has come of age, there have been technological developments on many levels, including transformation methods, control of gene expression, protein targeting and accumulation, the use of different crops as production platforms (Twyman et al , 2003), and modifications to alter the structural and functional properties of the product. One of the most important driving factors has been yield improvement, as product yield has a significant impact on economic feasibility. Strategies to improve the recombinant protein yield in plants include the development of novel promoters, the improvement of protein stability and accumulation through the use of signals that target the protein to intracellular compartments, and the improvement of downstream processing technologies (Menkhaus et al , 2004). Attention is now shifting from basic research towards commercial exploitation, and molecular farming is reaching the stage at which it could challenge established production technologies that use bacteria, yeast and cultured mammalian cells. …

Interactive 3-D Video Representation and Coding Technologies

Abstract: Interactivity in the sense of being able to explore and navigate audio-visual scenes by freely choosing viewpoint and viewing direction, is an important key feature of new and emerging audio-visual media. This paper gives an overview of suitable technology for such applications, with a focus on international standards, which are beneficial for consumers, service providers, and manufacturers. We first give a general classification and overview of interactive scene representation formats as commonly used in computer graphics literature. Then, we describe popular standard formats for interactive three-dimensional (3-D) scene representation and creation of virtual environments, the virtual reality modeling language (VRML), and the MPEG-4 BInary Format for Scenes (BIFS) with some examples. Recent extensions to MPEG-4 BIFS, the Animation Framework eXtension (AFX), providing advanced computer graphics tools, are explained and illustrated. New technologies mainly targeted at reconstruction, modeling, and representation of dynamic real world scenes are further studied. The user shall be able to navigate photorealistic scenes within certain restrictions, which can be roughly defined as 3-D video. Omnidirectional video is an extension of the planar two-dimensional (2-D) image plane to a spherical or cylindrical image plane. Any 2-D view in any direction can be rendered from this overall recording to give the user the impression of looking around. In interactive stereo two views, one for each eye, are synthesized to provide the user with an adequate depth cue of the observed scene. Head motion parallax viewing can be supported in a certain operating range if sufficient depth or disparity data are delivered with the video data. In free viewpoint video, a dynamic scene is captured by a number of cameras. The input data are transformed into a special data representation that enables interactive navigation through the dynamic scene environment.

Reporting guidelines for controlled experiments in software engineering

Abstract: One major problem for integrating study results into a common body of knowledge is the heterogeneity of reporting styles: (1) it is difficult to locate relevant information and (2) important information is often missing. Reporting guidelines are expected to support a systematic, standardized presentation of empirical research, thus improving reporting in order to support readers in (1) finding the information they are looking for, (2) understanding how an experiment is conducted, and (3) assessing the validity of its results. The objective of this paper is to survey the most prominent published proposals for reporting guidelines, and to derive a unified standard that which can serve as a starting point for further discussion. We provide detailed guidance on the expected content of the sections and subsections for reporting a specific type of empirical studies, i.e., controlled experiments. Before the guidelines can be evaluated, feedback from the research community is required. For this purpose, we propose to adapt guideline development processes from other disciplines.

Bridging the physical and digital in pervasive gaming

Abstract: A new generation of entertainment technology takes computer games to the streets---and ultimately beyond.

Endosperm-specific co-expression of recombinant soybean ferritin and Aspergillus phytase in maize results in significant increases in the levels of bioavailable iron.

Abstract: We have generated transgenic maize plants expressing Aspergillus phytase either alone or in combination with the iron-binding protein ferritin. Our aim was to produce grains with increased amounts of bioavailable iron in the endosperm. Maize seeds expressing recombinant phytase showed enzymatic activities of up to 3 IU per gram of seed. In flour paste prepared from these seeds, up to 95% of the endogenous phytic acid was degraded, with a concomitant increase in the amount of available phosphate. In seeds expressing ferritin in addition to phytase, the total iron content was significantly increased. To evaluate the impact of the recombinant proteins on iron absorption in the human gut, we used an in vitro digestion/Caco-2 cell model. We found that phytase in the maize seeds was associated with increased cellular iron uptake, and that the rate of iron uptake correlated with the level of phytase expression regardless of the total iron content of the seeds. We also investigated iron bioavailability under more complex meal conditions by adding ascorbic acid, which promotes iron uptake, to all samples. This resulted in a further increase in iron absorption, but the effects of phytase and ascorbic acid were not additive. We conclude that the expression of recombinant ferritin and phytase could help to increase iron availability and enhance the absorption of iron, particularly in cereal-based diets that lack other nutritional components.

Trapping single molecules by dielectrophoresis.

Abstract: We have trapped single protein molecules of R-phycoerythrin in an aqueous solution by an alternating electric field. A radio frequency voltage is applied to sharp nanoelectrodes and hence produces a strong electric field gradient. The resulting dielectrophoretic forces attract freely diffusing protein molecules. Trapping takes place at the electrode tips. Switching off the field immediately releases the molecules. The electric field distribution is computed, and from this the dielectrophoretic response of the molecules is calculated using a standard polarization model. The resulting forces are compared to the impact of Brownian motion. Finally, we discuss the experimental observations on the basis of the model calculations.

Architectural Patterns Revisited - A Pattern Language

Abstract: Architectural patterns are a key concept in the field of software architecture: they offer well-established solutions to architectural problems, help to document the architectural design decisions, facilitate communication between stakeholders through a common vocabulary, and describe the quality attributes of a software system as forces. Regrettably, finding and applying the appropriate architectural patterns in practice still remains largely ad-hoc and unsystematic. This is due to the lack of consensus in the community with respect to the “philosophy” and granularity of architectural patterns, as well as the lack of a coherent pattern language. In this paper we attempt to establish common ground in the architectural patterns community by proposing a pattern language that acts as a superset of the existing architectural pattern collections and categorizations. This language is particularly focused on establishing the relationships between the patterns and performs a categorization based on the concept of “architectural views”.

The G Protein-coupled Receptor Gpr1 and the Gα Protein Gpa2 Act through the cAMP-Protein Kinase A Pathway to Induce Morphogenesis in Candida albicans

Abstract: We investigated the role in cell morphogenesis and pathogenicity of the Candida albicans GPR1 gene, encoding the G protein-coupled receptor Gpr1. Deletion of C. albicans GPR1 has only minor effects in liquid hypha-inducing media but results in strong defects in the yeast-to-hypha transition on solid hypha-inducing media. Addition of cAMP, expression of a constitutively active allele of the Galpha protein Gpa2 or of the catalytic protein kinase A subunit TPK1 restores the wild-type phenotype of the CaGPR1-deleted strain. Overexpression of HST7, encoding a component of the mitogen-activated protein kinase pathway, does not suppress the defect in filamentation. These results indicate that CaGpr1 functions upstream in the cAMP-protein kinase A (PKA) pathway. We also show that, in the presence of glucose, CaGpr1 is important for amino acid-induced transition from yeast to hyphal cells. Finally, as opposed to previous reports, we show that CaGpa2 acts downstream of CaGpr1 as activator of the cAMP-PKA pathway but that deletion of neither CaGpr1 nor CaGpa2 affects glucose-induced cAMP signaling. In contrast, the latter is abolished in strains lacking CaCdc25 or CaRas1, suggesting that the CaCdc25-CaRas1 rather than the CaGpr1-CaGpa2 module mediates glucose-induced cAMP signaling in C. albicans.

Personalization and Context Management

Abstract: Supporting the individual user in his working, learning, or information access is one of the main goals of user modeling. Personal or group user models make it possible to represent and use information about preferences, knowledge, abilities, emotional states, and many other characteristics of a user to adapt the user experience and support. Nowadays, the disappearing computer enables the user to access her information from a variety of personal and public displays and devices. To support a new generation of contextualized and personalized information and services, this paper addresses the problem of context management. Context management is a new approach to the design of context-aware systems in ubiquitous computing that combines personalization and contextualization. The presented framework for context management integrates user modeling and context modeling, which can benefit from each other and give rise to more valid models for personalized and contextualized information delivery. The paper will introduce a base framework and tools for designing context-aware applications and decompose the underlying framework into its foundational components. As two illustrative application cases, the paper discusses implementations of an intelligent advertisement board and an audio-augmented museum environment.

Stress-induced single-polarization single-transverse mode photonic crystal fiber with low nonlinearity

Abstract: We report on the design of a single-polarization single-transverse mode large mode area photonic crystal fiber. By including index-matched stress applying elements in the photonic cladding an ultra-broadband single polarization window is obtained while a large mode field area of ~700 microm(2) is maintained. Based on that design, an Yb-doped double-clad photonic crystal fiber is realized that combines low nonlinearity and single polarization properties. A first result of the high power operation using this fiber is demonstrated.

An environmentally relevant concentration of estrogen induces arrest of male gonad development in zebrafish, Danio rerio

Abstract: The aim of the present study was to elucidate how full life-cycle exposure to estrogens impacts zebrafish development and reproduction, compared to partial life-cycle exposure only, and whether the estrogen-induced effects in zebrafish are reversible or irreversible. Zebrafish were exposed in a flow-through system to an environmentally relevant concentration (3 ng/L) of the synthetic estrogen 17alpha-ethinylestradiol (EE2) either from fertilization until the all-ovary stage of gonad development (i.e., 42 d postfertilization [DPF] in our experiment) or from fertilization until the reproductive stage (i.e., 118 DPF). Reversibility of the estrogen-induced effects was assessed after 58 d of depuration in EE2-free water until 176 DPE Early life exposure led to a lasting induction of plasma vitellogenin (VTG) in adult females but altered neither the sex ratio nor the reproductive capabilities. Full life-cycle exposure resulted in elevated VTG concentrations and caused gonadal feminization in 100% of exposed fish and thus inhibited reproduction. Two types of ovaries were observed in continuously exposed adult fish, immature ovaries with primary growth stage oocytes only and mature ovaries containing the full range of all oocyte maturation stages. Fish with immature ovaries had plasma VTG levels like control males, while fish with mature ovaries had female-like VTG levels. The effects of full life cycle exposure were at least partly reversible, and 26% of fish of the previous all-female cohort developed fully differentiated testes. These findings suggest that continuous estrogen exposure had arrested the developmental transition of the gonads of genetic males from the early all-ovary stage to functional testes. After the exposure had ceased, however, these males apparently were able to accomplish testicular differentiation.

The renaissance and bright future of fibre lasers

Abstract: The first rare-earth-doped fibre lasers were operated in the early 1960s and produced a few milliwatts at a wavelength around 1 µm. For the next several decades, fibre lasers were little more than a low-power laboratory curiosity. Recently, however, fibre lasers have entered the realm of kilowatt powers with diffraction-limited beam quality. In this paper we review the reasons for this power evolution. Beyond this, we will discuss how the next generation of fibres, so-called photonic crystal fibres, enable upward power scaling and therefore open up the avenue to new performance levels of solid-state lasers.