Instituto Superior Técnico

About: Instituto Superior Técnico is a based out in . It is known for research contribution in the topics: Catalysis & Finite element method. The organization has 10085 authors who have published 30226 publications receiving 667524 citations. The organization is also known as: IST & Instituto Superior Tecnico.

Specific engagement of the CD94/NKG2‐A killer inhibitory receptor by the HLA‐E class Ib molecule induces SHP‐1 phosphatase recruitment to tyrosine‐phosphorylated NKG2‐A: evidence for receptor function in heterologous transfectants

Abstract: It has been recently demonstrated that the CD94/NKG2-A killer inhibitory receptor (KIR) specifically recognizes the HLA-E class Ib molecule. Moreover, the apparent CD94-mediated specific recognition of different HLA class Ia allotypes, transfected into the HLA-defective cell line 721.221, indeed depends on their selective ability to concomitantly stabilize the surface expression of endogenous HLA-E molecules, which confer protection against CD94/NKG2-A+ effector cells. In the present study, we show that a selective engagement of the CD94/NKG2-A inhibitory receptor with a specific monoclonal antibody (mAb) (Z199) was sufficient to induce tyrosine phosphorylation of the NKG2-A subunit and SHP-1 recruitment. These early biochemical events, commonly related to negative signaling pathways, were also detected upon the specific interaction of NK cells with an HLA-E+ 721.221 transfectant (.221-AEH), and were prevented by pre-incubation of .221-AEH with an anti-HLA class I mAb. Furthermore, mAb cross-linking of the CD94/NKG2-A receptor, segregated from other NK-associated molecules by transfection into a rat basophilic leukemia cell line (RBL-2H3), promoted tyrosine phosphorylation of NKG2-A and co-precipitation of SHP-1, together with an inhibition of secretory events triggered via Fc epsilonRI. Remarkably, interaction of CD94/NKG2-A+ RBL cells with the HLA-E+ .221-AEH transfectant specifically induced a detectable association of SHP-1 with NKG2-A, constituting a more formal evidence for the receptor-HLA class I interaction.

Optimization of a Morphing Wing Based on Coupled Aerodynamic and Structural Constraints

Abstract: This paper presents the work done in designing a morphing wing concept for a small experimental unmanned aerial vehicle to improve the vehicle's performance over its intended speed range. The wing is designed with a multidisciplinary design optimization tool, in which an aerodynamic shape optimization code coupled with a structural morphing model is used to obtain a set of optimal wing shapes for minimum drag at different flight speeds. The optimization procedure is described as well as the structural model. The aerodynamic shape optimization code, that uses a viscous two-dimensional panel method formulation coupled with a nonlinear lifting-line algorithm and a sequential quadratic programming optimization algorithm, is suitable for preliminary wing design optimization tasks. The morphing concept, based on changes in wing-planform shape and wing-section shape achieved by extending spars and telescopic ribs, is explained in detail. Comparisons between optimized fixed wing performance, optimal morphing wing performance, and the performance of the wing obtained from the coupled aerodynamic-structural solution are presented. Estimates for the performance enhancements achieved by the unmanned aerial vehicles when fitted with this new morphing wing are also presented. Some conclusions on this concept are addressed with comments on the benefits and drawbacks of the morphing mechanism design.