Showing papers by "University of Kentucky published in 1989"
TL;DR: A method is presented for choosing optimal oligodeoxyribonucleotides as probes for filter hybridization, primer for sequencing, or primers for DNA amplification, based on a simple dynamic algorithm.
Abstract: A method is presented for choosing optimal oligodeoxyribonucleotides as probes for filter hybridization, primers for sequencing, or primers for DNA amplification. Three main factors that determine the quality of a probe are considered: stability of the duplex formed between the probe and target nucleic acid, specificity of the probe for the intended target sequence, and self-complementarity. DNA duplex stability calculations are based on the nearest-neighbor thermodynamic values determined by Breslauer et al. [Proc. Natl. Acad. Sci. U.S.A. (1986), 83: 3746]. Temperatures of duplex dissociation predicted by the method described here were within 0.4 degrees C of the values obtained experimentally for ten oligonucleotides. Calculations for specificity of the probe and its self-complementarity are based on a simple dynamic algorithm.
783 citations
TL;DR: Monocytes from alcoholic hepatitis patients have significantly increased spontaneous and lipopolysaccharide‐stimulated tumor necrosis factor release compared to monocytes from healthy volunteers, suggesting that some of the metabolic abnormalities and possibly some ofThe liver injury of alcoholic hepatitis may be due to enhanced tumor Necrosis factor production.
539 citations
TL;DR: The authors explored possible connections between gender and the willingness to engage in unethical business behavior and found that men are more than two times as likely as women to commit unethical business behaviors. But relatively few would engage in any of these actions with the exception of buying stock with inside information.
Abstract: This paper explores possible connections between gender and the willingness to engage in unethical business behavior. Two approaches to gender and ethics are presented: the structural approach and the socialization approach. Data from a sample of 213 business school students reveal that men are more than two times as likely as women to engage in actions regarded as unethical but it is also important to note that relatively few would engage in any of these actions with the exception of buying stock with inside information. Fifty percent of the males were willing to buy stock with insider information. Overall, the results support the gender socialization approach.
536 citations
TL;DR: Interactions between growth factors and neurotransmitters may be important in brain development; imbalances in these systems may lead to neurodegeneration; and cellular calcium-regulating systems may be a common focus of growth factor and neurotransmitter actions.
Abstract: Neuritic regression and cell death (neurodegeneration) are common features of both normal nervous system development and neurodegenerative disorders. Growth factors and excitatory amino acid neurotransmitters have been suggested independently to play roles in neurodegenerative processes. The present study investigated the combined effects of fibroblast growth factor (FGF) and glutamate on the development and degeneration of cultured hippocampal neurons. Consistent with previous data, we found that FGF, but not NGF, promoted neuronal survival and dendritic outgrowth. In contrast, a low level of glutamate (50 microM) caused a reduction in dendritic outgrowth, and high levels (100 microM-1 mM) reduced neuronal survival in a dose-dependent manner. When cultures were maintained in the presence of FGF, there was a striking reduction in neuronal death normally caused by 100-500 microM glutamate. FGF raised the threshold for glutamate neurotoxicity. FGF also antagonized the outgrowth-inhibiting actions of glutamate. Measurements of intracellular calcium levels with fura-2 demonstrated a direct relationship between glutamate-induced rises in intracellular calcium and neurodegeneration. FGF reduced the glutamate-induced increases in intracellular calcium levels. However, when cultures were pretreated with the RNA synthesis inhibitor actinomycin D or with the protein synthesis inhibitor cycloheximide, FGF did not prevent glutamate-induced increases in intracellular calcium or neurodegeneration. Taken together, these results suggest that (1) interactions between growth factors and neurotransmitters may be important in brain development; (2) imbalances in these systems may lead to neurodegeneration; and (3) cellular calcium-regulating systems may be a common focus of growth factor and neurotransmitter actions.
509 citations
TL;DR: A new approach to both the identification of membrane skeletal proteins in fibroblasts and changes that occur upon transformation by an activated tyrosine kinase is offered.
Abstract: We have previously reported the production of monoclonal antibodies directed against phosphotyrosine, which is the modification induced by many oncogene products and growth factor receptors. One of these antiphosphotyrosine antibodies (py20) was used in affinity chromatography to isolate phosphotyrosine (PY)-containing proteins from Rous sarcoma virus-transformed chick embryo fibroblasts (RSV-CEFs). Mice were immunized with these PY-proteins for the production of monoclonal antibodies to individual substrates. Fifteen antibodies were generated in this way to antigens with molecular masses of 215, 76, 60, and 22 kD. Antibodies to individual substrates were used to analyze the subcellular location in normal and RSV-CEFs. Antibodies to the 215- and 76-kD antigen stained focal contacts when used in immunofluorescence microscopy while anti-22-kD protein antibodies resulted in punctate staining concentrated in the margins of cells and in parallel arrays. Both distributions were altered in transformed cells. When cells were extracted with nonionic detergent under conditions that stabilize the cytoskeleton, 50% of the 76-kD protein and greater than 90% of the 22-kD protein fractionated with the cytoskeleton. This study offers a new approach to both the identification of membrane skeletal proteins in fibroblasts and changes that occur upon transformation by an activated tyrosine kinase.
453 citations
TL;DR: In this paper, the authors examined Williamson's (1975) concept of opportunism in relations between farm and power equipment dealers and their primary supplier organization and obtained results from a national survey.
Abstract: This study examines Williamson's (1975) concept of opportunism in relations between farm and power equipment dealers and their primary supplier organization. Results from a national survey generall...
423 citations
23 Mar 1989
422 citations
TL;DR: Vasoactive intestinal peptide labeled with 125I, [Tyr10-125I]VIP, can be hydrolyzed by immunoglobulin G (IgG) purified from a human subject, as judged by trichloroacetic acid precipitation and reversed-phase high-performance liquid chromatography (HPLC).
Abstract: Vasoactive intestinal peptide (VIP) labeled with 125I, [Tyr10-125I]VIP, can be hydrolyzed by immunoglobulin G (IgG) purified from a human subject, as judged by trichloroacetic acid precipitation and reversed-phase high-performance liquid chromatography (HPLC). The hydrolytic activity was precipitated by antibody to human IgG, it was bound by immobilized protein G and showed a molecular mass close to 150 kilodaltons by gel filtration chromatography, properties similar to those of authentic IgG. The Fab fragment, prepared from IgG by papain treatment, retained the VIP hydrolytic activity of the IgG. Peptide fragments produced by treatment of VIP with the antibody fraction were purified by reversed-phase HPLC and identified by fast atom bombardment-mass spectrometry and peptide sequencing. The scissile bond in VIP deduced from these experiments was Gln16-Met17. The antibody concentration (73.4 fmol per milligram of IgG) and the Kd (0.4 nM) were computed from analysis of VIP binding under conditions that did not result in peptide hydrolysis. Analysis of the antibody-mediated VIP hydrolysis at varying concentrations of substrate suggested conformity with Michaelis-Menton kinetics (Km). The values for Km (37.9 X 10(-9) M) and the turnover number kcat (15.6 min-1) suggested relatively tight VIP binding and a moderate catalytic efficiency of the antibody.
394 citations
TL;DR: In patients with mitral regurgitation, maximal jet area from Doppler color flow imaging provides a simple measurement that predicts angiographic grade, but manifests a weak correlation with regurgitant volume and does not predict hemodynamic dysfunction.
391 citations
TL;DR: In this paper, the authors examine pet ownership and pet attachment as factors supporting the health of the elderly, a national probability sample of Americans 65 years of age and older was drawn.
Abstract: In order to examine pet ownership and pet attachment as factors supporting the health of the elderly, a national probability sample of Americans 65 years of age and older was drawn. Participants answered telephone survey questions regarding pet ownership, life stress, social support, depression, and recent illness. In multiple regression analyses, pet ownership failed to predict depression and illness behavior, while pet attachment significantly predicted depression but not illness experience. In a group with particularly great distress (the bereaved), pet ownership and strong attachment were significantly associated with less depression only when the number of available confidants was minimal.
378 citations
TL;DR: The 22-kDa protein becomes an excellent candidate for a mediator of transformation by the tyrosine kinase class of oncogenes.
TL;DR: In this paper, statistical power regression analyses were performed on 19 sets of published data on size and laboratory permeability of unconsolidated sediments, and the exponent of grain diameter ranged from 1.11 to 2.05.
Abstract: Previous theoretical and empirical investigations of the relation between particle size and intergranular permeability have resulted in the well-known formula for intrinsic permeability (k)
where d is particle diameter, and c is a dimensionless constant. Statistical power regression analyses were per- formed on 19 sets of published data on size and laboratory permeability of unconsolidated sediments. The exponent of grain diameter ranged from 1.11 to 2.05, but most values were significantly less than 2.0. Results indicate that the permeability/grain size relation alternatively may be expressed, from an empirical basis, as
Values of c and the exponent both generally decrease with decreased textural maturity and increased induration.
Book•
01 Jan 198901 Jan 1989
TL;DR: Computer simulation studies are presented which demonstrate significantly improved reconstructed images achieved by an ART algorithm as compared to IRR methods.
Abstract: The author presents an algebraic reconstruction technique (ART) as a viable alternative in computerized tomography (CT) from limited views. Recently, algorithms of iterative reconstruction-reprojection (IRR) based on the method of convolution-backprojection have been proposed for application in limited-view CT. Reprojection was used in an iterative fashion alternating with backprojection as a means of estimating projection values within the sector of missing views. In algebraic methods of reconstruction for CT, only those projections corresponding to known data are required. Reprojection along missing views would merely serve to introduce redundant equations. Computer simulation studies are presented which demonstrate significantly improved reconstructed images achieved by an ART algorithm as compared to IRR methods. >
TL;DR: In this article, it was shown that radiated emissions due to common-mode currents on printed circuit board lands can greatly exceed those due to differential mode currents on wirelessly connected wires.
Abstract: It is shown that radiated emissions due to common-mode currents on printed circuit board lands can greatly exceed those due to differential-mode currents. It is concluded that predictions of radiated emissions based solely on differential-mode (transmission line) currents can bear little, if any, resemblance to actual measured emissions. Therefore, radiated emission prediction models that use only differential-mode (transmission-line) currents and ignore common-mode (antenna) currents are not adequate for the prediction of radiated emissions from printed circuit boards (as well as cables). >
TL;DR: In this paper, a multi-layered thin shell coupled with an active distributed vibration actuator was proposed and evaluated, where the dynamics and control of distributed mechanical systems were derived based on Love's theory and Hamilton's principle.
TL;DR: This paper applied Correspondence analysis to tourist perception data on Singapore and other Pacific Rim countries to improve Singapore's positioning in the tourism market and discussed how the Singapore Tourist Promotion Board can use the resulting output to improve the tourist market.
Abstract: Multinational tourism research involves analysis of multiple origins, multiple tourist destinations, and multiple attributes for destination selection . For example, tourist perceptions of a destination may vary across countries of origin. This can have important implications for which countries to target with tourism promotion, or which of the destination's attributes should be stressed in the promotion. Correspondence analysis is a technique which can handle problems of this complexity where other multiattribute analytical methods cannot. This technique is applied to empirical tourist perception data on Singapore and other Pacific Rim countries. A discussion of how the Singapore Tourist Promotion Board can use the resulting output to improve Singapore's positioning in the tourism market is presented.
TL;DR: It is proved that Σn is isomorphic to the boundary complex of some (n − 3)-dimensional simplicial convexpolytope, and that this polytope can be geometrically realized to have the dihedral group Dn as its group of symmetries.
TL;DR: Signals are writing devices that emphasize aspects of a text's content or structure without adding to the content of the text as discussed by the authors, and have been shown to influence reading and writing instruction.
Abstract: Signals are writing devices that emphasize aspects of a text's content or structure without adding to the content of the the text. Findings are reviewed for studies of several different types of signaling devices, including: titles, headings, previews, overviews, summaries, typographical cues, recall sentences, number signals, importance indicators, and summary indicators. Most investigations have examined how the presence of signals in a text affects subsequent memory for the text. Virtually all types of signals produce better memory for information they cue in a text, whereas memory for unsignaled information often is unaffected. Less attention has been directed to signaling effects on other cognitive processes, such as attention, basic reading processes, and comprehension. It is argued that an understanding of how signals influence these processes will contribute to the application of signaling research to reading and writing instruction and to our general understanding of reading.
TL;DR: It is suggested that admission hyperglycemia is a frequent component of the stress response to head injury, a significant indicator of severity of injury, and a significant predictor of outcome from head injury.
Abstract: Severe head injury is associated with a stress response that includes hyperglycemia, which has been shown to worsen outcome before or during cerebral ischemia. To better define the relationship between human head injury and hyperglycemia, glucose levels were followed in 59 consecutive brain-injured patients from hospital admission up to 18 days after injury. The patients who had the highest peak admission 24-hour serum glucose levels had the worse 18-day neurologic outcome (p = 0.01). Patients with peak 24-hour admission glucose levels greater than 200 mg/dL had a two-unit increase in Glasgow Coma Scale score while patients with admission peak 24-hour serum glucose levels less than or equal to 200 mg/dL had a four-unit increase in Glasgow Coma Scale score during the 18-day study period (p = 0.04). There was a significant relationship between 3-month and 1-year outcome and peak admission 24-hour serum glucose level (p = 0.02 and p = 0.02, respectively). Those patients with admission peak 24-hour serum glucose levels less than or equal to 200 mg/dL had a greater percentage of favorable outcome at 18 days, 3 months, and 1 year than those with admission peak 24-hour glucose levels greater than 200 mg/dL (p = 0.0007, p = 0.03, and p = 0.005, respectively). A significant relationship between admission peak 24-hour Glasgow Coma Scale score and 18-day, 3-month, and 1-year outcomes was found (p = 0.0001, p = 0.0002, and p = 0.0002, respectively). Patients with mean admission peak 24-hour Glasgow Coma Scale scores of 3.5, 6, and 10 had mean admission 24-hour peak serum glucose levels of 252 +/- 23.5, 219.1 +/- 19, and 185.8 +/- 21, respectively (p = 0.05). These relationships were not significantly altered when confounding variables such as the amount of glucose given over the initial 24-hour postinjury period, the presence of diabetes or multiple injuries, and whether patients were given steroids, dilantin, or insulin were statistically incorporated. These data suggest that admission hyperglycemia is a frequent component of the stress response to head injury, a significant indicator of severity of injury, and a significant predictor of outcome from head injury.
TL;DR: The physiological and biochemical adaptations that enable submersed aquatic macrophytes to manage constraints associated with photosynthesis under water are detailed, including their plasticity, which is seen in variable CO2 compensation points that indicate the photorespiratory (PR) state.
TL;DR: Plasma with LA was to a variable extent capable of decreasing or abolishing factor Va inhibition in normal plasma and exogenous activated protein C failed to correct the ineffective factor Va destruction despite adequate protein S levels, suggesting that LA prevents the formation of the complex essential for rapid proteolysis of factor Va both on phospholipid and on the platelet membrane.
TL;DR: Rats exposed to PEMF for 4 h/day for 7 days before crush, followed by 3 days after crush without PemF, also showed significantly increased regeneration, which suggests that P EMF influences regeneration indirectly.
TL;DR: 1,25(OH)2D3 treatment was associated with a significant fall in serum phosphorus and alkaline phosphatase concentrations as well as with histological evidence of an amelioration of hyperparathyroid changes, and no deterioration of renal function attributable to the treatment occurred.
TL;DR: Peat has been studied in several geologic settings: (1) glaciated terrain in cold temperate Maine and Minnesota, U.S.A., (2) an island in the Atlantic Ocean off the coast of Maine, where sea level is rising; (3) the warm temperate United States Atlantic and Gulf Coastal Plains, and (4) the tropical coast of Sarawak, Malaysia, and the tropical delta of the Batang Hari River, Sumatra, Indonesia.
TL;DR: Overall, rate and efficiency of gain were improved by feeding 125 or 250 ppm Cu as CuSO4, with the 125 ppm dietary level being about 75% as effective in stimulating growth as 250 ppm.
Abstract: Five 28- to 33-d experiments involving 460 crossbred pigs weaned at 28 +/- 2 d of age (initial weight, 6.7 to 8.1 kg) were conducted to determine the effects of feeding high dietary levels of Cu sulfate (CuSO4) or Cu oxide (CuO) on rate and efficiency of gain and liver Cu stores of weanling pigs. The pigs were housed in groups of five to six/pen and fed a fortified, unmedicated, corn-soybean meal-dried whey basal diet (1.1% lysine, 30 ppm Cu). In Exp. 1 and 2, pigs (eight replicates) were fed the basal or the basal plus 125 or 250 ppm Cu from CuSO4 or CuO for 28 d. In Exp. 3 and 4, four replications were fed the same diets as in Exp. 1 and 2 plus two additional diets (500 ppm Cu from CuSO4 or CuO). In Exp. 5, dietary levels of 0, 125, 250, 375 or 500 ppm Cu from CuSO4 were evaluated using four replications. At the end of each experiment, the liver from one pig in each pen was collected for Cu analysis. Overall, rate and efficiency of gain were improved (P less than .01) by feeding 125 or 250 ppm Cu as CuSO4, with the 125 ppm dietary level being about 75% as effective in stimulating growth as 250 ppm. Performance of pigs was not different from controls when the highest (500 ppm) level of Cu (from CuSO4) was fed. Liver Cu increased 10- to 70-fold when 250 to 550 ppm Cu from CuSO4 was included in the feed.(ABSTRACT TRUNCATED AT 250 WORDS)
TL;DR: Results suggest that tobacco suspension cells accommodate the presence of non-growth-inhibiting and growth- inhibiting levels of Cd and Zn by sequestration in the vacuole as complexes with endogenous organic acids and that this may be a principal means for accommodation ofCd as well as Zn in the presence and absence of C d-peptide.
Abstract: Responses of tobacco (Nicotiana tabacum) suspension cells to Cd and Zn were studied in the presence and absence of ligand of Cd-peptide in order to understand the role of this peptide versus other mechanisms in Cd and Zn accumulation and accommodation in plants. With 45 micromolar Cd and 300 micromolar Zn (non-growth-inhibiting levels), metals appeared rapidly within cells, and intracellular Cd and Zn reached medium concentrations after 6 to 10 hours. Cd-peptide was observed in response to Cd after 2 hours, but this form only accounted for ∼30% of soluble Cd after 24 hours. Peptide was not observed in cells exposed to 300 micromolar Zn for up to 7 days. Organic acid-to-metal stoichiometry indicated that endogenous organic acid content of cells was more than sufficient to complex absorbed metals and no evidence was found for stimulation of organic acid biosynthesis by Cd or Zn. Metal-complexing potential of organic acids for Cd and Zn versus endogenous cations is discussed as is vacuolar-extravacuolar distribution of metals. The absence of Cd-peptide does not limit Cd-accumulation in the system studied. Results suggest that tobacco suspension cells accommodate the presence of non-growth-inhibiting and growth-inhibiting levels of Cd and Zn by sequestration in the vacuole as complexes with endogenous organic acids and that this may be a principal means for accommodation of Cd as well as Zn in the presence and absence of Cd-peptide.
TL;DR: The authors described the evolution of one researcher's work on the process of attitude and perceptual change among teachers and developed a theoretical model of teacher change, with implications for educational improvement efforts, and several questions related to teacher change and stemming from the model are discussed.