Institution
University of Maryland Biotechnology Institute
About: University of Maryland Biotechnology Institute is a based out in . It is known for research contribution in the topics: Gene & Population. The organization has 1565 authors who have published 2458 publications receiving 171434 citations. The organization is also known as: UMBI.
Topics: Gene, Population, Protein structure, Receptor, Peptide sequence
Papers published on a yearly basis
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TL;DR: The synthesis of oligomannose-containing glycoconjugates that include either a carrier protein or a universal T-helper epitope peptide to provide an effective immunogen is described.
127 citations
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TL;DR: It is suggested that covalently crosslinked complexes of the HIV-1 surface envelope glycoprotein and CD4 elicit broadly neutralizing humoral responses that, in part, may be directed against a novel epitope(s) found on theAIDS envelope.
Abstract: The identification of HIV envelope structures that generate broadly cross-reactive neutralizing antibodies is a major goal for HIV-vaccine development. In this study, we evaluated one such structure, expressed as either a gp120–CD4 or a gp140–CD4 complex, for its ability to elicit a neutralizing antibody response. In rhesus macaques, covalently crosslinked complexes of soluble human CD4 (shCD4) and HIV-1IIIB envelope glycoproteins (gp120 or gp140) generated antibodies that neutralized a wide range of primary HIV-1 isolates regardless of the coreceptor usage or genetic subtype. Ig with cross-reactive neutralizing activity was recovered by affinity chromatography with a chimeric single-chain polypeptide containing sequences for HIVBaL gp120 and a mimetic peptide that induces a CD4-triggered envelope structure. These results suggest that covalently crosslinked complexes of the HIV-1 surface envelope glycoprotein and CD4 elicit broadly neutralizing humoral responses that, in part, may be directed against a novel epitope(s) found on the HIV-1 envelope.
127 citations
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TL;DR: The three-dimensional structure of an unglycosylated T cell antigen receptor (TCR) beta chain has recently been determined and its affinity for various bacterial SAGs in the absence of MHC class II molecules is measured, finding the much weaker binding to SEB than to SEC1, 2, or 3 was surprising.
Abstract: The three-dimensional structure of an unglycosylated T cell antigen receptor (TCR) beta chain has recently been determined to 1.7 A resolution. To investigate whether this soluble beta chain (murine V beta 8.2J beta 2.1C beta 1) retains superantigen (SAG)-binding activity, we measured its affinity for various bacterial SAGs in the absence of MHC class II molecules. Dissociation constants (KDs) were determined using two independent techniques: surface plasmon resonance detection and sedimentation equilibrium. Specific binding was demonstrated to staphylococcal enterotoxins (SEs) B, C1, C2, and C3 and to streptococcal pyrogenic exotoxin A (SPEA), consistent with the known proliferative effects of these SAGs on T cells expressing V beta 8.2. In contrast, SEA, which does not stimulate V beta 8.2-bearing cells, does not bind the recombinant beta chain. Binding of the beta chain to SAGs was characterized by extremely fast dissociation rates (> 0.1 s-1), similar to those reported for certain leukocyte adhesion molecules. Whereas the beta chain bound SEC1, 2, and 3 with KDs of 0.9-2.5 microM, the corresponding value for SEB was approximately 140 microM. The much weaker binding to SEB than to SEC1, 2, or 3 was surprising, especially since SEB was found to actually be 3- to 10-fold more effective, on a molar basis, than the other toxins in stimulating the parental T cell hybridoma. We interpret these results in terms of the ability of SEC to activate T cells independently of MHC, in contrast to SEB. We have also measured SE binding to the glycosylated form of the beta chain and found that carbohydrate apparently does not contribute to recognition, even though the N-linked glycosylation sites at V beta 8.2 residues Asn24 and Asn74 are at or near the putative SAG-binding site. This result, along with the structural basis for the V beta specificity of SEs, are discussed in relation to the crystal structure of the unglycosylated beta chain.
127 citations
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TL;DR: It has become clear that a fundamental change of approach to the understanding and control of microbial diseases must be implemented and a comprehensive strategy is needed to elucidate the syntrophic associations that are essential for a healthy relation among microbes and between them and the host organism, and to unveil those associations that lead to disease.
126 citations
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Swiss Institute of Bioinformatics1, California Institute for Quantitative Biosciences2, Howard Hughes Medical Institute3, Stanford University4, Rutgers University5, University College London6, University of California, Berkeley7, University of Washington8, University of North Carolina at Chapel Hill9, Fox Chase Cancer Center10, University of California, Davis11, Albert Einstein College of Medicine12, Discovery Institute13, University of California, San Francisco14, Bristol-Myers Squibb15, University of Maryland Biotechnology Institute16, Columbia University17, Icahn School of Medicine at Mount Sinai18, University of Chicago19, Osaka University20, Boston University21, Scripps Research Institute22
TL;DR: The proceedings and conclusions from the "Workshop on Applications of Protein Models in Biomedical Research" (the Workshop) that was held at the University of California, San Francisco on 11 and 12 July, 2008 are described.
126 citations
Authors
Showing all 1565 results
Name | H-index | Papers | Citations |
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Stanley B. Prusiner | 168 | 745 | 97528 |
Robert C. Gallo | 145 | 825 | 68212 |
Thomas J. Smith | 140 | 1775 | 113919 |
J. D. Hansen | 122 | 975 | 76198 |
Stephen Mann | 120 | 669 | 55008 |
Donald M. Bers | 118 | 570 | 52757 |
Jon Clardy | 116 | 983 | 56617 |
Rita R. Colwell | 115 | 781 | 55229 |
Joseph R. Lakowicz | 104 | 850 | 76257 |
Patrick M. Schlievert | 90 | 444 | 32037 |
Mitsuhiko Ikura | 89 | 316 | 34132 |
Jeremy Thorner | 87 | 234 | 29999 |
Lawrence E. Samelson | 87 | 209 | 27398 |
Jacques Ravel | 86 | 323 | 45793 |
W. J. Lederer | 79 | 213 | 25509 |