Showing papers in "Journal of Immunological Methods in 2014"

TL;DR: The formal optimization and validation of the TZM-bl assay was performed in compliance with Good Clinical Laboratory Practice (GCLP) guidelines and the assay was evaluated for specificity, accuracy, precision, limits of detection and quantitation, linearity, range and robustness.

TL;DR: By crossing thirteen different myeloid-Cre mouse strains to ROSA-EYFP reporter mice and assaying YFP expression in a variety of naïve unstimulated hematopoietic cells, in parallel, this work directly compares the relative efficiency and specificity of myeloids deletion in these strains under steady-state conditions.

TL;DR: An optimized method for exosome isolation from human plasma/serum specimens of normal controls (NC) or cancer patients and its advantages and pitfalls are described, yielding biologically-active, morphologically-intact exosomes that have reasonably good purity without large protein losses.

TL;DR: This review will focus on the three animal models currently used most frequently in influenza virus research – mice, ferrets, and guinea pigs – and discuss the advantages and disadvantages of each.

TL;DR: Mice have been used as experimental models for S. aureus SSTI, bacteremia, sepsis, peritonitis and endocarditis, leading to the identification of key virulence factors, candidate vaccine antigens or immune-therapeutics that still require human clinical testing to establish efficacy.

TL;DR: The development of “next generation” humanized mouse models continues to provide exciting opportunities for the establishment of robust small animal models to study the pathogenesis of human-specific infectious agents, as well as for testing the efficacy of therapeutic agents and experimental vaccines.

TL;DR: This study provides methods to employ CyTOF for small samples, which is especially relevant for investigation of limited patient biopsies in translational and clinical research.

TL;DR: This work provides a new and effective method for the isolation of tumor-derived exosomes based on "charge neutralization" with acetate, and shows that titration of tissue culture supernatants with 0.1M acetate to pH4.75 results in immediate precipitation of virtually all the exosome.

TL;DR: It is demonstrated that two different digestion protocols yield significant differences in detection of CD4(+) and CD8(+) T cells, NK cells, monocytes and interdigitating DC populations, highlighting the importance and impact of cell collection protocols on assay outputs.

TL;DR: Results demonstrate that serum GMAb measurement using the GMAb ELISA was accurate, precise, reliable, had an acceptable LLOQ, and could be accurately expressed in standardized units, and introduce a new unit of measure to enable standardized reporting of serumGMAb data from different laboratories.

TL;DR: This revised protocol combines high transfection efficiency and cell vitality with a strong response to polarizing stimuli and therefore constitutes a suitable model for studying macrophage polarization in vitro.

TL;DR: It is proposed that non-specific binding may be interpreted as an informative finding indicative of elevated IgG and inflammation, and shown that the level of non- specific binding correlates with the IgG concentration and therefore is shown to be indicative of inflammation.

TL;DR: A robust reporter-based ADCC reporter assay that uses an engineered Jurkat stable cell line as the source of effector cells is developed and is able to measure antibody potency for several therapeutic antibodies targeted to hematopoietic or solid tumors.

TL;DR: The impedance-based assay represents a valuable and highly sensitive tool for label-free real-time high throughput analysis of T-cell-mediated cytotoxicity, and outperform the CRA both in reproducibility and sensitivity after prolonged incubation.

TL;DR: A decrease in concentration was observed during storage of both neurofilament proteins up to 21days at 37°C, which was significant by day 5, and both NfH and NfL are stable under the most prevalent pre-analytical variations.

TL;DR: This review outlines salient features of common vertebrate animal model systems to study fungal pathogenesis, host antifungal immune responses, and antIFungal compounds.

TL;DR: It is beneficial and is recommended to have a well-defined plan for the characterization of ADA domain specificity and data analysis prior to the initiation of sample testing, and best practices for immunogenicity evaluation of complex MDBs are discussed.

TL;DR: The results support the notion that there is a Ly6C(hi)-monocyte-directed pulmonary AMφ alternative activation and provide a dynamic view of mononuclear phagocyte change in three compartments after bleomycin challenge, relevant for designing new treatment strategies targeting monon nuclear phagocytes in this model.

TL;DR: Analysis of sequence diversity of functional variable and constant regions observed in 57 conventional heavy, 18 kappa and 35 lambda light chains of C. dromedarius and Camelus bactrianus finds designed oligonucleotide primers have enabled identification of paired heavy and light Chains of conventional camel antibodies from individual B cell clones.

TL;DR: The objective was to determine the effects of cryopreservation of effector PBMC on cell frequency, and on the magnitude and specificity of ADCC and NK activity.

TL;DR: This study evaluated the impact of different effector cell types including primary PBMCs, primary NK cells, engineered NK cell lines, and an engineered reporter cell line, on the in vitro ADCC activity of two glycoforms of a humanized IgG1 antibody to show the differential effects on both the efficacy and potency.

TL;DR: A multiplex electrochemiluminescent assay system is validated and found that it has superior test characteristics in human plasma compared to mouse BALF and plasma and compared favorably to well-validated singleplex ELISAs.

TL;DR: The EQAPOL Viral Diversity panel is an invaluable collection of well-characterized reagents available to the scientific community, including researchers, epidemiologists, and commercial manufacturers of diagnostics and pharmaceuticals to support HIV research, as well as diagnostic and vaccine development.

TL;DR: For the first time, it is shown that diagnostic NL should be performed only once daily to get comparable results, and storing at -70°C for ≥5 years led to significantly reduced mediator concentrations in NL compared to contemporary analyses.

TL;DR: This review focuses on summarizing recent advances obtained with well-established and more innovative experimental mouse models, giving emphasis on the strength of infection systems based on the reconstitution of the murine liver with human hepatocytes as tools for elucidating the whole life cycle of HBV and HDV.

TL;DR: It is demonstrated that Treg depletion in DEREG mice is transient and that DT administration can have adverse effects during virus-induced inflammation and highlights the critical need to include DT-treated WT mice when using DTR models to control for DT-mediated toxicity.

TL;DR: The optimization and validation of the HIV-1 neutralizing antibody assay that utilizes A3R5 cells is described, performed in compliance with Good Clinical Laboratory Practice (GCLP) guidelines, and employed as an endpoint immunogenicity test for vaccine-elicited neutralizing antibodies against tier 2 strains of HIV- 1.

TL;DR: A novel adaptation for culture of primary IEC is described for human duodenal organ donor tissue as well as duodenum and colon of adult mice to allow for more appropriate studies of IECs as innate immune effectors.

TL;DR: It is found that immediately after Nucleofection®, CD4 T cells undergo dramatic morphological changes characterized by wrinkled and dilated plasma membranes before recovering 1h later, and transcriptional activity is increased in the first 24h after N nucleofection, even in the absence of exogenous stimuli.

TL;DR: The majority of Ig concentrations recovered from 108 newborns of the Upstate KIDs Study were within the range of newborn plasma Ig levels with the exception of IgG3, which displayed the greatest variance with a wide range.