Showing papers in "Molecular and Biochemical Parasitology in 1992"

TL;DR: The sequence of a region of the rapidly evolving mitochondrial genome is useful as a marker of species and strain identity and as a preliminary indication of evolutionary divergence within the genus Echinococcus.

TL;DR: Based on the observed point-mutations an identification of the Leishmania parasites could be achieved by direct sequencing, restriction fragment analysis or single-stranded conformation polymorphism of the PCR-generated fragments.

TL;DR: It is proposed that T. cruzi intracellular development is critically dependent on GP57/51 (cruzipain), and selective inhibitors for this cysteinyl proteinase may have therapeutic potential.

TL;DR: Primary processing of MSP1(42) occurs in preparations of washed, disrupted merozoites, and is inhibited by the protease inhibitors phenylmethylsulphonyl fluoride (PMSF) and diisopropyl fluorophosphate (DFP), indicating that the prote enzyme responsible is a membrane-associated serine protease.

TL;DR: It is demonstrated that cruzipain, the major cysteine proteinase of Trypanosoma cruzi epimastigotes, is encoded by a large number of tandemly arranged genes, and the sequence is conserved among the repeat units, although polymorphisms clearly exist.

TL;DR: The monoclonal antibody Tg49 both recognizes a Toxoplasma gondii rhoptry protein (ROP1) and inhibits penetration enhancing factor, and it seems likely that the ROP1 protein is a component of that factor.

TL;DR: Proteins encoded by this B. bovis gene family have been designated variable merozoite surface antigens (VMSA), and the extent and mechanism of VMSA polymorphism among strains will be important when evaluating the role these surface proteins have in the host-parasite interaction, including immunity to blood stages.

TL;DR: Using nonradioactive hybridization, the human multilocus probe 33.15 was shown to recognize multiple minisatellite regions in nuclear DNA from Trypanosoma cruzi, producing complex banding patterns on Southern blots, typical of DNA fingerprints.

TL;DR: A genetic linkage map of the Plasmodium falciparum genome is reported, using the inheritance patterns of nearly 90 RFLP markers in a genetic cross to suggest certain parental linkage groups on chromosomes 2, 3, 12 and 13 were favored in the cross.

TL;DR: A sensitive and specific polymerase chain reaction-based assay has been developed to detect and analyze polymorphism in the Giardia lamblia 18S ribosomal RNA gene and revealed the presence of the different sequence types in these specimens.

TL;DR: The sex-specific expression of alpha-tubulin II and its localization to axoneme of the male parasite suggest a role for this molecule in the morphologic changes that occur during exflagellation and in the motility of the parasite.

TL;DR: Results strongly suggest that the P. falciparum cysteine proteinase gene isolated and characterized encodes TCP, which appears to be an essential malarial hemoglobinase and a potential target for antimalarial chemotherapy.

TL;DR: An arsenical resistant cloned line of Trypanosoma brucei Brucei was derived from a parent sensitive clone by repeated selection in vivo with the pentavalent melaminophenyl arsenical, sodium melarsen, and found to be cross-resistant to the trivalent arsenicals.

TL;DR: The energy metabolism of the plant parasite Phytomonas sp.

TL;DR: The availability of recombinant DNA-derived Pv200 proteins will now allow a full assessment of their utility in the diagnosis and immunoprophylaxis of the benign tertian malaria associated with P. vivax infection.

TL;DR: There is no direct metabolic interface between arginine and glucose catabolism in Giardia intestinalis, and the activities of these enzymes are up to 250 fold greater than those reported for the enzymes in Trichomonas vaginalis.

TL;DR: A molecular karyotype based on separation of chromosomes by pulsed field gel electrophoresis and assignment of linkage groups by hybridization is constructed and should greatly aid in using genetics to study the biology, drug resistance, and virulence of this important opportunistic pathogen.

TL;DR: An actin gene from a Cryptosporidium parvum genomic DNA library is identified and isolated using a chicken beta-actin cDNA as an hybridization probe and the nucleotide sequences of two overlapping recombinant clones were identical.

TL;DR: Antisera directed against the major Sm28GST epitopes revealed differences in the recognition of the 28-kDa GSTs from the other schistosome species suggesting that these regions have been subjected to evolutionary pressure.

TL;DR: This study has identified further naturally occurring processing sites and a consensus processing site sequence is now emerging, which was the only mAb which significantly inhibited parasite growth in vitro.

TL;DR: Comparison of the effects of sialidase and influenza C virus esterase treatments of mouse erythrocytes on invasion and the binding of a 175-kDa P. falciparum protein implicated in the invasion process indicates that sialic acid is part of a receptor for invasion.

TL;DR: Quantitation of the G RA4 gene and Southern blot analysis suggested that the GRA4 gene is single copy, which indicates a proline rich product with an internal hydrophobic region of 19 amino acids and a potential site of N-glycosylation.

TL;DR: Recombinant p67 is a candidate antigen for development of an anti-sporozoite vaccine for East Coast fever in cattle and has been expressed in Escherichia coli as a fusion protein with Sj-26, a glutathione-S-transferase of Schistosoma japonicum.

TL;DR: In this paper, a complete copy of an eggshell protein precursor gene, p48, was identified and characterized, showing that the gene has three open reading frames and does not contain an intron.

TL;DR: Results indicated that p33 and p34 were conserved molecules among these Theileria species, and the genes that encode p33/34 proteins were suitable for discrimination of T. buffeli/T.

TL;DR: Experimental evidence indicates that tubulin is the site of action of the anthelmintic benzimidazoles and certain residues of β-tubulin seem to be critical for this mechanism, and several regions of consistent difference can be recognized.