Showing papers in "Veterinary Microbiology in 2021"

TL;DR: Two serologically and molecularly non-typeable isolates of the porcine lung pathogen Actinobacillus pleuropneumoniae have been identified from diseased swine in two different continents as discussed by the authors.

TL;DR: It is demonstrated that the respiratory tissues of cattle and sheep, but not of pigs, sustain viral replication in vitro of both isolates and that SARS-CoV-2 binds efficiently to ACE2-expressing cells of the respiratory tract of both ruminant species.

TL;DR: Accumulated ROS played an essential role in regulating ER stress-mediated autophagy during PEDV infection, and activation of ER stress triggered the unfolded protein response (UPR) through PERK, IRE1, and ATF6 pathways during P EDV infection.

TL;DR: In this article, the authors showed that ASFV MGF505-11R interacted with STING, degrading STING expression by the lysosomal, ubiquitin-proteasome and autophagy pathways.

TL;DR: A review focusing on microorganisms and microbial-derived products that could provide a naturally evolved solution to ETEC infection and disease is presented in this paper, where the authors focus on bacteriophage therapy, probiotics and the use of probiotic fermentation products as postbiotics.

TL;DR: In this article, a China SVA isolate (CH-LX-01-2016) was rescued from its cDNA clone, and then identified by RT-PCR, indirect immunofluorescence assay and mass spectrometry.

TL;DR: In this paper, a combined Oxford Nanopore (ONT) long-reads and Illumina short-reads sequencing approach was used to characterize an E. coli ST167 isolated from a hospitalized dog, and the plasmid containing blaNDM-5 was fully reconstructed.

TL;DR: More efforts are needed to evaluate the clinical impact of these tigecycline resistance genes, as their activities on tetracyclines were determined by microbiological degradation and mass spectrometry, followed by susceptibility testing, sequence typing, gene transfer, molecular location and genomic DNA sequencing analyses.

TL;DR: The results suggested that PEDV E protein acts as an IFN-β antagonist through suppression of the RIG-I-mediated signaling.

TL;DR: In this paper, a review of the characteristics and differences in clinical signs, lesions, viral replication and tropism between virulent and classical PRRSV strains and a first approximation to the criteria to define a virulent strain was proposed.

TL;DR: In this article, the interaction between autophagy and PDCoV replication in LLC-PK1 cells was investigated, and ergosterol peroxide (EP) alleviated the activation of p38 signaling pathway to trigger autophagia, thus exerting its antiviral effects.

TL;DR: In this paper, the expression of circ_0050463, which is predominantly localized in cytoplasm, in response to IAV infection, was analyzed, and it was shown that circ_50463 functioned as an endogenous microRNA sponge to sequester and inhibit miR-33b-5p activity, resulting in increased eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) expression with subsequent facilitation of IAV replication.

TL;DR: Investigation of the prevalence and antimicrobial susceptibility of Clostridium perfringens in chickens and pigs in Beijing and Shanxi provided scientific basis for efficient intervention or prevention measures of antimicrobial resistance in animal husbandry in China.

TL;DR: In this article, a vaccine candidate strain in which the protein kinase UL13 gene was deleted was constructed with the CRISPR/Cas9 system based on the recombinant pseudorabies virus (PRV) ZJ01-ΔgI/gE/TK.

TL;DR: Wang et al. as mentioned in this paper conducted whole-transcriptome RNA-seq analysis in porcine alveolar macrophages (PAMs) infected with pig/Heilongjiang/2018 (Pig/HLJ/18) ASFV at different time points.

TL;DR: In this paper, the authors investigated the infection of SARS-CoV-2 in pets (18 cats and 20 dogs) from owners previously confirmed as COVID-19-positive.

TL;DR: Interestingly, ISChh1-like were also found mediating integration of other antibiotic resistance genes in Campylobacter, including tet(O), aphA3 and aadE-sat4-aphA3 gene cluster.

TL;DR: In this paper, the presence and quantity of microorganisms by culture and quantitative real-time qPCR was determined in calves with acute bovine respiratory disease (BRD) in 11 herds geographically spread over Denmark.

TL;DR: In this article, the preventive effects of FMR feeding on calf diarrhea using an experimental infection model of bovine rotavirus (BRV) in newborn calves and a field study in dairy farms with calf diarrhea were verified.

TL;DR: Individual deletion of these IMP genes from the ORFV genome resulted in slight reduction in virulence in vivo, as evidenced by a reduction in the duration of the clinical disease and virus shedding.

TL;DR: A longitudinal molecular screening of domestic cats infected with domestic cat hepadnavirus (DCH) was performed to determine if DCH can cause chronic infections in cats as discussed by the authors, which is consistent with the relatively high number of viremic cats identified in epidemiological investigations, with the possible association of DCH with chronic hepatic pathologies and with what described with HBV in humans.

TL;DR: In this paper, the effects of different types of bile acids on the replication of porcine deltacoronavirus (PDCoV) in cell culture were investigated.

TL;DR: Results suggest that O108:HNM-A-ST10 (CH11-24) F18 is an emerging clone taking space left by other classical serogroups in pig colibacillosis, and is essential for the update of vaccines, as alternative to the use of antibiotics.

TL;DR: Wang et al. as mentioned in this paper investigated the specific mechanism of severe pulmonary inflammation caused by G. parasuis infection, and provided a foundation for further elucidating the pathogenic mechanism.

TL;DR: Although differences in the infection kinetics and sequences of these six isolates were found, clinical presentation of vesicular disease was similar between both historical and contemporary isolate, and ease of experimental reproduction of disease with contemporary strains of SVA was consistently demonstrated experimentally.

TL;DR: In this paper, a multi-locus sequence typing scheme for the characterization of Mammaliicoccus (formerly Staphylococcus) sciuri was developed, which includes the analysis of internal segments of the housekeeping genes ack, aroE, ftsZ, glpK, gmk, pta1 and tpiA.

TL;DR: In this paper, the authors constructed five SVA minigenomes with point mutations in the pseudoknot stem I and II motif, which is composed of two base-paired motifs (PKS-Ia and -Ib), between which there is an unpaired spacing (UpS).

TL;DR: In this article, a deletion mutant of the MGF100-1R gene was constructed based on ASFV Chinese strain GZ201801, which led to a similar pathogenesis that caused by the parental AS FV GZ 201801.

TL;DR: Zhang et al. as mentioned in this paper found that PCV2 infection suppressed IFN-β expression and promoted porcine parvovirus (PPV) infection in piglets.

TL;DR: In this article, the analysis of serum samples for WNV-specific antibodies by ELISA, whose specificity was subsequently confirmed by virus-neutralization test (VNT) showed positive results in 18.23 % birds belonging to 18 different species.