Journal ArticleDOI
A dipyrrole derivative from Aloe vera inhibits an anti-diabetic drug target Dipeptidyl Peptidase (DPP)-IV in vitro.
TLDR
The results of the studies suggested that the inhibition of the DPP-IV enzyme as one of the pathways by which the Aloe vera extract may restore the pancreatic islets cell mass in diabetic animal model.Abstract:
Aloe vera, a succulent herb, has a long history of use in traditional medicine, including diabetes. Earlier studies from our laboratory demonstrated that the Aloe vera extract has the ability to inhibit the diabetic drug target dipeptidyl peptidase (DPP) IV in vitro. This current study focuses on the isolation of small water soluble active molecule(s) involved in DPP-IV inhibition from Aloe vera extract, and further to characterize its structure and to elucidate the mode of inhibition of the DPP-IV enzyme. Aloe vera gel ethanolic extract was subjected to preparative reverse-phase high-pressure liquid chromatography (RP-HPLC), LH-20 Sephadex gel filtration chromatography, followed by analytical RP-HPLC, to isolate the active molecule involved in DPP-IV inhibition. Based on the spectroscopic studies, the structure of the isolated DPP-IV inhibitor was predicted to be 3, 6-dioxo-3, 3a, 6, 6 a-tetrahydropyrrolo [3, 4-c] pyrrole-1, 4-dicarboxamide with the chemical formula C8H6N4O4, having the molecular weight of 225.175 Da. This molecule inhibited the DPP-IV enzyme in a noncompetitive manner with an IC50 value of 8.59 ± 2.61 µM, with a Ki of 4.7 ± 0.038 µM. Thus, the mechanism of DPP-IV inhibition and the inhibitory constants were determined. The results of our studies suggested that the inhibition of the DPP-IV enzyme as one of the pathways by which the Aloe vera extract may restore the pancreatic islets cell mass in diabetic animal model.read more
Citations
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Journal ArticleDOI
Identification of Potential SARS-CoV-2 Main Protease and Spike Protein Inhibitors from the Genus Aloe : An In Silico Study for Drug Development.
Mohamed E. Abouelela,Hamdy K. Assaf,Reda A. Abdelhamid,Ehab S Elkhyat,Ahmed M. Sayed,Tomasz Oszako,Lassaad Belbahri,Ahmed E. El Zowalaty,Mohamed Salaheldin A. Abdelkader +8 more
TL;DR: In this article, the authors employed an in-silico approach involving molecular docking, dynamics simulation, and binding free energy calculation using SARS-CoV-2 essential proteins as main protease and spike protein to identify lead compounds from Aloe that may help in novel drug discovery.
Journal ArticleDOI
Dipeptidyl Peptidase (DPP)-IV Inhibitors with Antioxidant Potential Isolated from Natural Sources: A Novel Approach for the Management of Diabetes
TL;DR: In this article, a review of DPP-IV inhibitors and their mechanism of inhibition, activities of those isolated from various natural sources, and their capacity to overcome oxidative stress in disease conditions is presented.
Journal ArticleDOI
Alleviation of diabetes mellitus through the restoration of β-cell function and lipid metabolism by Aloe vera (L.) Burm. f. extract in obesogenic WNIN/GR-Ob rats.
Neha Deora,M.M. Sunitha,M. Satyavani,N. Harishankar,Mookambeswaran A. Vijayalakshmi,Krishnan Venkataraman,Vijayalakshmi Venkateshan +6 more
TL;DR: In this article, the authors investigated the simultaneous effect of ethanolic A. vera gel extract on diabetes and obesogenic milieu in Streptozotocin-induced WNIN/GR-Ob mutant obese rats.
Journal ArticleDOI
Antidiabetic potential evaluation of aqueous extract of waste Syzygium cumini seed kernel’s by in vitro α-amylase and α-glucosidase inhibition
TL;DR: The output of this research bestows support to utilize the SCKP stirred batch extract as a promising source of antioxidant and antidiabetic compounds in ayurvedic formulations.
Journal ArticleDOI
Downregulation of Candidate Gene Expression and Neuroprotection by Piperine in Streptozotocin-Induced Hyperglycemia and Memory Impairment in Rats.
Suresh Kumar,Suman Chowdhury,Ajay Razdan,Deepa Kumari,Ram Singh Purty,Heera Ram,Pramod Kumar,Prasunpriya Nayak,Sunil Shukla +8 more
TL;DR: In this paper, the authors evaluated the effect of piperine on Alzheimer's disease in diabetic rats and showed that piperines not only improved memory but also reduced the expression of specific AD-related genes.
References
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Journal ArticleDOI
Antifungal activity in vitro of Aloe vera pulp and liquid fraction against plant pathogenic fungi
TL;DR: The results showed an inhibitory effect of the pulp of A. Vera on F. Vera, the first report of any Aloe liquid fraction activity against plant pathogenic fungi.
Journal ArticleDOI
Cardiovascular Effects of New Oral Glucose-Lowering Agents: DPP-4 and SGLT-2 Inhibitors.
TL;DR: In this article, a review of the effects of new oral glucose-lowering agents for cardiovascular disease (CVD) is devoted to the effect of these drugs on patients with type 2 diabetes mellitus.
Journal ArticleDOI
DPP-4 Inhibitors: Impact on glycemic control and cardiovascular risk factors
TL;DR: The different DPP-4 inhibitors are distinctive in their metabolism, their excretion, their recommended dosage, and the daily dosage that is required for effective treatment, however, when comparing their efficacy regarding lowering HbA1c levels, safety profile, and patient tolerance they are similar.
Journal Article
Antidiabetic activity of Aloe vera and histology of organs in streptozotocin-induced diabetic rats
TL;DR: In diabetic rats and diabetic rats fed with A. vera, no change was noticed in the kidney and stomach, while in streptozotocin-induced diabetic rats, the fasting plasma glucose levels were reduced to normal and body weight was found to be increased.
Journal ArticleDOI
In vitro inhibition of dipeptidyl peptidase IV by peptides derived from the hydrolysis of amaranth (Amaranthus hypochondriacus L.) proteins.
Aída J. Velarde-Salcedo,Alberto Barrera-Pacheco,Samuel Lara-González,Gabriela M. Montero-Morán,Agustín Diaz-Gois,Elvira Gonzalez de Mejia,Ana P. Barba de la Rosa +6 more
TL;DR: This is the first report of the identification of inhibitory DPPIV peptides from amaranth hydrolysates and the prediction of their binding modes at the molecular level, leading to their possible use as functional food ingredients in the prevention of diabetes.