A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)
Yu-Chi Peng,Chia-Hsuan Cheng,Hiromi Yatsuda,Szu-Heng Liu,Shih-Jen Liu,Takashi Kogai,Chen-Yen Kuo,Robert Wang +7 more
- Vol. 11, Iss: 10, pp 1838
TLDR
In this article, a shear-horizontal surface acoustic wave (SH-SAW) biosensor was developed to detect the anti-SARS-CoV-2 nucleocapsid antibody.Abstract:
Since the Coronavirus disease 2019 (COVID-19) pandemic outbreak, many methods have been used to detect antigens or antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including viral culture, nucleic acid test, and immunoassay. The shear-horizontal surface acoustic wave (SH-SAW) biosensor is a novel pathogen detection platform with the advantages of high sensitivity and short detection time. The objective of this study is to develop a SH-SAW biosensor to detect the anti-SARS-CoV-2 nucleocapsid antibody. The rabbit sera collected from rabbits on different days after SARS-CoV-2 N protein injection were evaluated by SH-SAW biosensor and enzyme-linked immunosorbent assay (ELISA). The results showed that the SH-SAW biosensor achieved a high correlation coefficient (R = 0.9997) with different concentrations (34.375–1100 ng/mL) of the “spike-in” anti-N protein antibodies. Compared to ELISA, the SH-SAW biosensor has better sensitivity and can detect anti-N protein IgG signals earlier than ELISA on day 6 (p < 0.05). Overall, in this study, we demonstrated that the SH-SAW biosensor is a promising platform for rapid in vitro diagnostic (IVD) testing, especially for antigen or antibody testing.read more
Citations
More filters
Journal ArticleDOI
Measurements of Anti-SARS-CoV-2 Antibody Levels after Vaccination Using a SH-SAW Biosensor
Chia-Hsuan Cheng,Yu-Chi Peng,Shu Min Lin,Hiromi Yatsuda,Szu-Heng Liu,Shih-Jen Liu,Chen Yen Kuo,Robert Y L Wang +7 more
TL;DR: SH-SAW sensors in vitro diagnostic systems are a simple and powerful technology to investigate the local prevalence of COVID-19 and found that the total anti-SARS-CoV-2 S protein antibody concentrations spiked 10–14 days after the first vaccination.
Journal ArticleDOI
Trends and Applications of Surface and Bulk Acoustic Wave Devices: A Review
TL;DR: In this paper , the authors report and discuss the key basic principles of SAW and BAW devices and their typical geometries and electrical characterization methodology, and discuss recent promising studies that pave the way for the exploitation of these elastic wave devices for new applications that fit into current challenges.
Journal ArticleDOI
Design and simulation of a millifluidic device for differential detection of SARS-CoV-2 and H1N1 based on triboelectricity
Tara Ghafouri,Negin Manavizadeh +1 more
TL;DR: In this article , a label-free, self-powered, and ultrafast immunosensor device working based on triboelectric effect is proposed, where equilibrium constants of specific antibody-antigen reactions are accompanied by IEP-relevant electric charges of antigens to recognize SARS CoV-2 and H1N1.
Posted ContentDOI
Urinary SARS-CoV-2 RNA is An Indicator For The Progression and Prognosis of COVID-19 Disease
Maoqing Tian,Lu Zhang,Kai Zhu,Bo Shen,Gang Wang,Yuan Song,Cheng Chen,Wei Liang,Yang Guan,Guohua Ding,Tiechi Lei,Xiaogang Li,Jingyuan Xie,Yongqing Tong,Huiming Wang +14 more
TL;DR: Wang et al. as mentioned in this paper analyzed the clinical characteristics and laboratory tests of COVID-19 patients and detected SARS-CoV-2 RNA in urine sediments collected from 53 patients enrolled in Renmin Hospital of Wuhan University from 31 January 2020 to 18 February 2020 with qRT-PCR analysis.
Journal ArticleDOI
The Recent Development of Acoustic Sensors as Effective Chemical Detecting Tools for Biological Cells and Their Bioactivities
TL;DR: In this paper , the use of acoustic wave sensors for in vivo visualizing and imaging of animal and plant cellular chemical compositions could significantly help pave the way toward advanced analytical technologies. But the authors focus on the application of these wave sensors in biomedical and microfluidic media progress.
References
More filters
Journal ArticleDOI
Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding.
Roujian Lu,Xiang Zhao,Juan Li,Peihua Niu,Bo Yang,Honglong Wu,Wenling Wang,Hao Song,Baoying Huang,Na Zhu,Yuhai Bi,Xuejun Ma,Faxian Zhan,Liang Wang,Tao Hu,Hong Zhou,Zhenhong Hu,Weimin Zhou,Li Zhao,Jing Chen,Yao Meng,Ji Wang,Yang Lin,Jianying Yuan,Zhihao Xie,Jinmin Ma,William J. Liu,Dayan Wang,Wenbo Xu,Edward C. Holmes,George F. Gao,George F. Gao,Guizhen Wu,Weijun Chen,Weifeng Shi,Wenjie Tan,Wenjie Tan +36 more
TL;DR: The phylogenetic analysis suggests that bats might be the original host of this virus, an animal sold at the seafood market in Wuhan might represent an intermediate host facilitating the emergence of the virus in humans.
Journal ArticleDOI
Characteristics of SARS-CoV-2 and COVID-19
TL;DR: The basic virology of SARS-CoV-2 is described, including genomic characteristics and receptor use, highlighting its key difference from previously known coronaviruses.
Journal ArticleDOI
Development and Clinical Application of A Rapid IgM-IgG Combined Antibody Test for SARS-CoV-2 Infection Diagnosis
Zhengtu Li,Yongxiang Yi,Xiaomei Luo,Nian Xiong,Yang Liu,Shaoqiang Li,Ruilin Sun,Yanqun Wang,Bicheng Hu,Wei Chen,Yongchen Zhang,Jing Wang,Huang Baofu,Ye Lin,Jiasheng Yang,Wensheng Cai,Wang Xuefeng,Jing Cheng,Zhiqiang Chen,Kangjun Sun,Pan Weimin,Zhifei Zhan,Liyan Chen,Feng Ye +23 more
TL;DR: A rapid and simple point‐of‐care lateral flow immunoassay that can detect immunoglobulin M (IgM) and IgG antibodies simultaneously against SARS‐CoV‐2 virus in human blood within 15 minutes which can detect patients at different infection stages is developed.
Journal ArticleDOI
Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection.
Jacqueline Dinnes,Jacqueline Dinnes,Jonathan J Deeks,Ada Adriano,Sarah Berhane,Clare Davenport,Sabine Dittrich,Devy Emperador,Yemisi Takwoingi,Jane Cunningham,Sophie Beese,Janine Dretzke,Lavinia Ferrante di Ruffano,Isobel Marion Harris,Malcolm J Price,Sian Taylor-Phillips,Lotty Hooft,Mariska M.G. Leeflang,René Spijker,Ann Van den Bruel +19 more
TL;DR: Assessment of the diagnostic accuracy of point‐of‐care antigen and molecular‐based tests to determine if a person presenting in the community or in primary or secondary care has current SARS‐CoV‐2 infection found no studies at low risk of bias for all quality domains and concerns about applicability of results across all studies.
Journal ArticleDOI
Dual-Functional Plasmonic Photothermal Biosensors for Highly Accurate Severe Acute Respiratory Syndrome Coronavirus 2 Detection.
Guangyu Qiu,Guangyu Qiu,Zhibo Gai,Zhibo Gai,Yile Tao,Yile Tao,Jean Schmitt,Jean Schmitt,Gerd A. Kullak-Ublick,Gerd A. Kullak-Ublick,Jing Wang,Jing Wang +11 more
TL;DR: Insight is gained into the thermoplasmonic enhancement and its applicability in the nucleic acid tests and viral disease diagnosis and an alternative and promising solution for the clinical COVID-19 diagnosis is provided.