A rapid chemical method for quantification of lipids separated by thin-layer chromatography.
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In this article, thin-layer chromatography was used to quantify all lipid classes to a lower limit of 15 μg of lipid, where the reduction in absorbance at 350 mμ is proportional to the amount of lipid.About:
This article is published in Journal of Lipid Research.The article was published on 1964-04-01 and is currently open access. It has received 369 citations till now. The article focuses on the topics: Thin-layer chromatography & Reagent.read more
Citations
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Simple charring method for determination of lipids
TL;DR: A rapid method is described for charring 5-300 micrograms of lipids (with concentrated sulfuric acid in a test tube) and estimating them with a reproducibility of +/-1%.
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Lipid storage in marine zooplankton
TL;DR: The various functions storage lipids serve during the life history stages of zooplankton are very complex and still not fully understood and hence offer a multitude of fascinating research perspectives.
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Complete separation of lipid classes on a single thin-layer plate.
C.P. Freeman,D. West +1 more
TL;DR: A double-development procedure employing first a polar and then a nonpolar solvent system is described for the complete separation by thin-layer chromatography of the main lipid classes encountered in natural lipids.
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Relationship between essential fatty acid requirements of aquatic animals and the capacity for bioconversion of linolenic acid to highly unsaturated fatty acids.
TL;DR: Linolenic acid was converted to 20:5 omega 3 and 22:6 omega 3 intensively in the rainbow trout, moderately in the ayu, eel and prawn, but slightly in the red sea bream, rockfish and globefish.
References
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Über die quantitative Auswertung von Dünnschichtchromatogrammen der Cholesterinester
TL;DR: In this paper, the authors untersucht, ob eine quantileve Auswertung dee durch Besprfihen mit Antimontr ichlor id and anschlieBendes Erhi tzen entstehenden Farbkomplexes m5glich ist.
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Untersuchungen über die Dünnschichtchromatographic von Lipoiden
N Zoellner,Günther Wolfram +1 more
TL;DR: In this paper, die grundsatzlichen Moglichkeiten zur chromatographischen Trennung der Lipoide an der Dunnschicht werden besprochen und an Beispielen erlautert.
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Quantitative measurement of the amine buffer: 2-amino-2-hydroxymethyl-1,3-propanediol.
TL;DR: Two variations of the method will be described, the more sensitive depending on the ultraviolet absorption peaks as absorption maxima of the CrzOe form are chosen further into the ultraviolet, for example, 350 and 255 mfi.