A rapid chemical method for quantification of lipids separated by thin-layer chromatography.
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In this article, thin-layer chromatography was used to quantify all lipid classes to a lower limit of 15 μg of lipid, where the reduction in absorbance at 350 mμ is proportional to the amount of lipid.About:
This article is published in Journal of Lipid Research.The article was published on 1964-04-01 and is currently open access. It has received 369 citations till now. The article focuses on the topics: Thin-layer chromatography & Reagent.read more
Citations
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Ethanol consumption increases nitric oxide production in rats, and its peroxynitrite-mediated toxicity is attenuated by polyenylphosphatidylcholine.
TL;DR: Chronic, but not acute, ethanol administration increases peroxynitrite hepatotoxicity by enhancing concomitant production of nitric oxide and superoxide, both of which are prevented by polyenylphosphatidylcholine.
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Alleviation of abiotic salt stress in Ochradenus baccatus (Del.) by Trichoderma hamatum (Bonord.) Bainier
TL;DR: Application of bioagent (T. hamatum) has been observed to alleviate the antagonistic effect of salt stress on plant growth and metabolic processes.
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The influence of adult diet and age on lipid reserves in the tropical fruit fly Anastrepha serpentina (Diptera: Tephritidae)
TL;DR: Longevity and egg production were lower in flies kept on diets of S and S+IF than on the other diets, and Lipid reserves were similar in both sexes fed the same diet.
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Ethanol Oxidation by a Component of Liver Microsomes Rich in Cytochrome P-450
TL;DR: It is concluded that ethanol oxidation can be catalyzed by a microsomal component rich in cytochrome P-450 and separate from contaminating alcohol dehydrogenase and catalase.
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Factors affecting the exchange of tocopherol between red blood cells and plasma.
John G. Bieri,R P Evarts,S Thorp +2 more
TL;DR: Of possible clinical significance was the finding that the RBC: plasma ratio was related to the total lipid concentration of plasma, indicating that the red cell was not saturated up to four times normal plasma levels.
References
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A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
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Colorimetric determination of blood lipides.
TL;DR: The determinations reported here followed a conventional alcohol-ether extraction and subsequent reextraction in petroleum ether, and it has been found that urea, even in amounts considerably in excess of what might occur, produces no color change in the dichromate reagent.
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Quantitative estimation of esters by thin-layer chromatography
TL;DR: In this article, a method for the analysis of fatty esters employing separation by thin-layer chromatography and quantitative determination via their hydroxamic acids has been developed, which is suitable for analysis of mixtures of ordinary, epoxy, monohydroxy, and dihydroxy fatty acids.
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Rapid thin-layer chromatographic separation of phospholipids and neutral lipids of serum.
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Quantitative Estimation of Some Phosphatides and Their Hydrolysis Products by Thin Layer Chromatography.
William M. Doizaki,Leslie Zieve +1 more
TL;DR: The results are similar to those obtained with silicic acid impregnated paper chromatography and column techniques, though the time for the analysis is markedly shortened.