A rapid chemical method for quantification of lipids separated by thin-layer chromatography.
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In this article, thin-layer chromatography was used to quantify all lipid classes to a lower limit of 15 μg of lipid, where the reduction in absorbance at 350 mμ is proportional to the amount of lipid.About:
This article is published in Journal of Lipid Research.The article was published on 1964-04-01 and is currently open access. It has received 369 citations till now. The article focuses on the topics: Thin-layer chromatography & Reagent.read more
Citations
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Lipid droplet formation during vitellogenesis in the cecropia moth
TL;DR: Autoradiograms confirmed that the deposition of lipid droplets like that of the protein yolk spheres occurs in the peripherial cytoplasm of the oocyte, adjacent to the follicular epithelium.
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Effect of fatty acid saturation on the distribution of the cholesterol moiety of very low density lipoproteins.
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Lipid content and fatty acid composition of heart and muscle of the BIO 82.62 cardiomyopathic hamster
TL;DR: Analysis of the fatty acid composition of triglycerides showed that the cardiomyopathic hamsters store more saturated fatty acids in both heart and muscle than do their normal counterparts, which may imply that the desaturation mechanism is altered in the diseased animals.
Impact of plant growth promoting bacillus subtilis on growth and physiological parameters of bassia indica (indian bassia) grown udder salt stress
TL;DR: The salt-tolerant PGPR, B. subtilis could act synergistically to promote the growth and fitness of Indian bassia plants under salt stress by providing an additional supply of an auxin (IAA) and induce salt stress resistance by reducing stress ethylene levels.
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Synthesis and release of sulfolipid by Mycobacterium avium during growth andcell division.
TL;DR: Results demonstrated that the isotope was not lost to the cells because of turnover, but rather that the labeled compounds were released, intact, from the cells after fission.
References
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A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
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Colorimetric determination of blood lipides.
TL;DR: The determinations reported here followed a conventional alcohol-ether extraction and subsequent reextraction in petroleum ether, and it has been found that urea, even in amounts considerably in excess of what might occur, produces no color change in the dichromate reagent.
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Quantitative estimation of esters by thin-layer chromatography
TL;DR: In this article, a method for the analysis of fatty esters employing separation by thin-layer chromatography and quantitative determination via their hydroxamic acids has been developed, which is suitable for analysis of mixtures of ordinary, epoxy, monohydroxy, and dihydroxy fatty acids.
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Rapid thin-layer chromatographic separation of phospholipids and neutral lipids of serum.
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Quantitative Estimation of Some Phosphatides and Their Hydrolysis Products by Thin Layer Chromatography.
William M. Doizaki,Leslie Zieve +1 more
TL;DR: The results are similar to those obtained with silicic acid impregnated paper chromatography and column techniques, though the time for the analysis is markedly shortened.