A rapid chemical method for quantification of lipids separated by thin-layer chromatography.
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In this article, thin-layer chromatography was used to quantify all lipid classes to a lower limit of 15 μg of lipid, where the reduction in absorbance at 350 mμ is proportional to the amount of lipid.About:
This article is published in Journal of Lipid Research.The article was published on 1964-04-01 and is currently open access. It has received 369 citations till now. The article focuses on the topics: Thin-layer chromatography & Reagent.read more
Citations
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Lipids from nerve tissues of the horseshoe crab, Limulus polyphemus
Richard F. Lee,Frances Gonsoulin +1 more
TL;DR: Cerebrosides and gangliosides, which are associated with vertebrate nerve tissues, were absent from nerves of horseshoe crabs and phospholipid fraction was characterized by a high content of plasmalogen.
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Carbohydrate-induced lipemia. report of a familial occurrence.
TL;DR: In this disease, designated as "fat-induced lipemia," lipoprotein lipase is present in decreased concentration, and the disorder is thought to represent impaired clearing from the blood of chylomicrons formed during the absorption of dietary triglyceride.
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The antioxidant and stress-protective properties of an extract from the green alga Ulva lactuca Linnaeus, 1753
TL;DR: The effects of an extract from the green alga Ulva lactuca and the Essentiale® commercial preparation on the weight indexes of the viscera and the biochemical markers of the liver have been studied in a model of acute stress in rats.
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The Influence of an Extract from the Marine Brown Alga Sargassum pallidum on the Metabolic Reactions in the Liver under Experimental Toxic Hepatitis
V. G. Sprygin,N. F. Kushnerova,S. E. Fomenko,E. S. Drugova,L. N. Lesnikova,V. Yu. Merzlyakov,T. V. Momot,T. V. Momot +7 more
TL;DR: The hepatoprotective effect of S. pallidum extract is associated with the action of polyphenols contained in brown algae, that is, phlorotannins, which are highly active antioxidants and restore the liver metabolic reactions after toxic hepatic injury with CTC.
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Dissection of Normal and Atherosclerotic Human Artery with Proteolytic Enzymes in vitro
TL;DR: Preliminary investigations indicate that controlled irrigation of arteries with proteolytic enzymes can partially satisfy the need for resolving the intima into mixed populations of cells in a graded manner.
References
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A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
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Colorimetric determination of blood lipides.
TL;DR: The determinations reported here followed a conventional alcohol-ether extraction and subsequent reextraction in petroleum ether, and it has been found that urea, even in amounts considerably in excess of what might occur, produces no color change in the dichromate reagent.
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Quantitative estimation of esters by thin-layer chromatography
TL;DR: In this article, a method for the analysis of fatty esters employing separation by thin-layer chromatography and quantitative determination via their hydroxamic acids has been developed, which is suitable for analysis of mixtures of ordinary, epoxy, monohydroxy, and dihydroxy fatty acids.
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Rapid thin-layer chromatographic separation of phospholipids and neutral lipids of serum.
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Quantitative Estimation of Some Phosphatides and Their Hydrolysis Products by Thin Layer Chromatography.
William M. Doizaki,Leslie Zieve +1 more
TL;DR: The results are similar to those obtained with silicic acid impregnated paper chromatography and column techniques, though the time for the analysis is markedly shortened.