A rapid chemical method for quantification of lipids separated by thin-layer chromatography.
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In this article, thin-layer chromatography was used to quantify all lipid classes to a lower limit of 15 μg of lipid, where the reduction in absorbance at 350 mμ is proportional to the amount of lipid.About:
This article is published in Journal of Lipid Research.The article was published on 1964-04-01 and is currently open access. It has received 369 citations till now. The article focuses on the topics: Thin-layer chromatography & Reagent.read more
Citations
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Lipid activation of undecaprenol kinase from Lactobacillus plantarum.
Jack R. Kalin,Charles M. Allen +1 more
TL;DR: Similar substrate specificity and kinetic constants were observed for both the solubilized and reconstituted enzymes suggesting that no fundamental changes in the enzyme activity occurred during the delipidation-reconstitution process.
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Fatty acid flux and triglyceride secretion in the hydrazine-induced fatty liver.
J.S. Amenta,A.M. Dominguez +1 more
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The influence of clofibrate on lipid turnover in mouse tissues.
TL;DR: It is established that clofibrate perturbs lipid metabolism in all the major tissues of this animal, and indicates that this response is not solely due to an increase in the number and oxidative capacity of peroxisomes.
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On the role of catalase in the oxidation of tissue fatty acids.
Denis I. Crane,Colin Masters +1 more
TL;DR: Results may be taken as indicating that interruption of the process of peroxisomal beta-oxidation in this manner cause extensive perturbation of lipid metabolism in the living animal, and these perturbations extend well beyond those tissues where the predominant localization of these organelles occurs.
References
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A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
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Colorimetric determination of blood lipides.
TL;DR: The determinations reported here followed a conventional alcohol-ether extraction and subsequent reextraction in petroleum ether, and it has been found that urea, even in amounts considerably in excess of what might occur, produces no color change in the dichromate reagent.
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Quantitative estimation of esters by thin-layer chromatography
TL;DR: In this article, a method for the analysis of fatty esters employing separation by thin-layer chromatography and quantitative determination via their hydroxamic acids has been developed, which is suitable for analysis of mixtures of ordinary, epoxy, monohydroxy, and dihydroxy fatty acids.
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Rapid thin-layer chromatographic separation of phospholipids and neutral lipids of serum.
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Quantitative Estimation of Some Phosphatides and Their Hydrolysis Products by Thin Layer Chromatography.
William M. Doizaki,Leslie Zieve +1 more
TL;DR: The results are similar to those obtained with silicic acid impregnated paper chromatography and column techniques, though the time for the analysis is markedly shortened.