Journal ArticleDOI
A similarity between viral defense and gene silencing in plants.
TLDR
In this article, it was found that nepovirus infection of nontransgenic plants induces a resistance mechanism that is similar to transgene-induced gene silencing, which may be related to natural defense against viruses.Abstract:
Gene silencing in plants, in which an endogenous gene is suppressed by introduction of a related transgene, has been used for crop improvement. Observations that viruses are potentially both initiators and targets of gene silencing suggested that this phenomenon may be related to natural defense against viruses. Supporting this idea, it was found that nepovirus infection of nontransgenic plants induces a resistance mechanism that is similar to transgene-induced gene silencing.read more
Citations
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Journal ArticleDOI
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Andrew Fire,SiQun Xu,Mary K. Montgomery,Steven A. Kostas,Steven A. Kostas,Samuel E. Driver,Craig C. Mello +6 more
TL;DR: To their surprise, it was found that double-stranded RNA was substantially more effective at producing interference than was either strand individually, arguing against stochiometric interference with endogenous mRNA and suggesting that there could be a catalytic or amplification component in the interference process.
Journal ArticleDOI
An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells
TL;DR: It is shown that ‘loss-of-function’ phenotypes can be created in cultured Drosophila cells by transfection with specific double-stranded RNAs, which coincides with a marked reduction in the level of cognate cellular messenger RNAs.
Journal ArticleDOI
A species of small antisense RNA in posttranscriptional gene silencing in plants.
TL;DR: The 25-nucleotide antisense RNA detected in transgene-induced PTGS is likely synthesized from an RNA template and may represent the specificity determinant of PTGS.
Journal ArticleDOI
RNA silencing in plants
TL;DR: Diverse biological roles of these pathways have been established, including defence against viruses, regulation of gene expression and the condensation of chromatin into heterochromatin, and the full extent of this functional diversity in genetic and epigenetic mechanisms of genome control is investigated.
Patent
Genetic Inhibition by Double-Stranded RNA
Andrew Fire,Stephen A. Kostas,Mary K. Montgomery,Lisa Timmons,SiQun Xu,Hiroaki Tabara,Samuel E. Driver,Craig C. Mello +7 more
TL;DR: In this article, a double-stranded RNA has been used to inhibit gene expression of a target gene in a living cell in order to identify the source and target genes in the cell.
References
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Journal ArticleDOI
A comprehensive set of sequence analysis programs for the VAX
TL;DR: A group of programs that will interact with each other has been developed for the Digital Equipment Corporation VAX computer using the VMS operating system.
Journal ArticleDOI
Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance.
TL;DR: It is proposed that the resistant state and reduced steady state levels of transgene transcript accumulation are mediated at the cellular level by a cytoplasmic activity that targets specific RNA sequences for inactivation.
Journal ArticleDOI
Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA.
Monto H. Kumagai,J Donson,Guy Della-Cioppa,Damon A. Harvey,Kathleen M. Hanley,Laurence K. Grill +5 more
TL;DR: This work demonstrates that an episomal RNA viral vector can be used to deliberately manipulate a major, eukaryotic biosynthetic pathway and indicates that an antisense transcript generated in the cytoplasm of a plant cell can turn off endogenous gene expression.
Journal ArticleDOI
How and Why Do Plants Inactivate Homologous (Trans)genes
TL;DR: Gene silencing in transgenic plants has emerged in the last 5 years as a topic of intense interest for both applied and basic plant scientists and a small cadre of basic scientists has become fascinated by the phenomenon and is analyzing a variety of silencing systems.
Journal ArticleDOI
Jellyfish green fluorescent protein as a reporter for virus infections
TL;DR: It is likely that GFP will be useful as a reporter gene in transgenic plants as well as in virus-infected tissue, and earlier speculation that the PVX coat protein is essential for cell-to-cell movement is confirmed.