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Accurate and universal delineation of prokaryotic species

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TLDR
The specI (species identification tool) as mentioned in this paper ) is a method to group organisms into species clusters based on 40 universal, single-copy phylogenetic marker genes, applied to 3,496 prokaryotic genomes, specI identified 1,753 species clusters.
Abstract
The exponentially increasing number of sequenced genomes necessitates fast, accurate, universally applicable and automated approaches for the delineation of prokaryotic species. We developed specI (species identification tool; http://www.bork.embl.de/software/specI/), a method to group organisms into species clusters based on 40 universal, single-copy phylogenetic marker genes. Applied to 3,496 prokaryotic genomes, specI identified 1,753 species clusters. Of 314 discrepancies with a widely used taxonomic classification, >62% were resolved by literature support.

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Comparative Genomics of a Parthenogenesis-Inducing Wolbachia Symbiont.

TL;DR: The genome sequence from a parthenogenesis-inducing Wolbachia strain (wTpre) infecting the minute parasitoid wasp Trichogramma pretiosum is presented and it is hypothesized that the large number of coding frame truncations has accompanied the change in reproductive mode of the wTpre strain.
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Evaluating techniques for metagenome annotation using simulated sequence data.

TL;DR: A simulated metagenome is used to show how different parameters affect annotation accuracy by evaluating the sequence annotation performances of MEGAN, MG-RAST, One Codex and Megablast and concludes that there is a trade-off between taxonomic resolution and annotation accuracy.
Journal ArticleDOI

Phylogeny of a relapsing fever Borrelia species transmitted by the hard tick Ixodes scapularis.

TL;DR: Comparisons of the several species of Borrelia, including those in the two major clades, showed that LB-2001 and related hard tick-associated organisms, likeborrelia lonestari, are deeply positioned within the RF group of species and that these organisms did not, as some earlier estimations had suggested, constitute a paraphyletic group.
Journal ArticleDOI

leBIBIQBPP: a set of databases and a webtool for automatic phylogenetic analysis of prokaryotic sequences.

TL;DR: leBIBIQBPP is a powerful tool helping biologists to position bacterial or archaeal sequence commonly used markers in a phylogeny using an approximate maximum likelihood approach and providing fully documented results to help the user in decision making.
References
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Journal ArticleDOI

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
Journal ArticleDOI

Search and clustering orders of magnitude faster than BLAST

Robert C. Edgar
- 01 Oct 2010 - 
TL;DR: UCLUST is a new clustering method that exploits USEARCH to assign sequences to clusters and offers several advantages over the widely used program CD-HIT, including higher speed, lower memory use, improved sensitivity, clustering at lower identities and classification of much larger datasets.
Book

Bergey's Manual of Systematic Bacteriology

TL;DR: BCL3 and Sheehy cite Bergey's manual of determinative bacteriology of which systematic bacteriology, first edition, is an expansion.
Journal ArticleDOI

RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models

TL;DR: UNLABELLED RAxML-VI-HPC (randomized axelerated maximum likelihood for high performance computing) is a sequential and parallel program for inference of large phylogenies with maximum likelihood (ML) that has been used to compute ML trees on two of the largest alignments to date.
Journal ArticleDOI

Improved tools for biological sequence comparison.

TL;DR: Three computer programs for comparisons of protein and DNA sequences can be used to search sequence data bases, evaluate similarity scores, and identify periodic structures based on local sequence similarity.
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