Journal ArticleDOI
An improvement in a procedure for counting tritium and carbon-14 in polyacrylamide gels.
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This article is published in Analytical Biochemistry.The article was published on 1970-05-01. It has received 73 citations till now. The article focuses on the topics: Polyacrylamide & Tritium.read more
Citations
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Journal ArticleDOI
Proteins and glycoproteins of hamster kidney fibroblast (bhk21) plasma membranes and endoplasmic reticulum
TL;DR: The apparent molecular weights of the major plasma membrane and endoplasmic reticulum glycoproteins were determined by polyacrylamide gel electrophoresis and were found to differ significantly.
Journal ArticleDOI
Reversible uncoupling of photophosphorylation by a new bifunctional maleimide.
J V Moroney,R E McCarty +1 more
TL;DR: Results suggest that cross-linking is the cause for the increased proton permeability in thylakoids treated with bifunctional maleimides in the light.
Journal ArticleDOI
Protein Degradation during Yeast Sporulation Enzyme and Cytochrome Patterns
Heinrich Betz,Ulrike Weiser +1 more
TL;DR: It is postulated that the meiotic events and the metabolic changes required for ascospore formation are under separate genetic control in this organism.
Journal ArticleDOI
Illumination of chloroplast thylakoids in the presence of ATP causes the binding of ADP to one of the large subunits of coupling factor 1
TL;DR: Prolonged digestion of CF1 with trypsin yields an active ATPase devoid of the three smaller subunits, suggesting that one of the two larger subunits contains this nucleotide binding site.
Journal ArticleDOI
An in vivo study of a nonstructural polypeptide synthesized upon TMV infection and its identification with a polypeptide synthesized in vitro from TMV RNA
TL;DR: Close similarity was found between hydrolysis products, suggesting that the sedimentable and the soluble in vivo , and the in vitro polypeptides are identical molecular species.
References
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Journal ArticleDOI
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels
Journal ArticleDOI
Molecular weight estimation of polypeptides by SDS-polyacrylamide gel electrophoresis: further data concerning resolving power and general considerations.
TL;DR: Experiments with S35-labeled SDS suggest that the SDS-protein complex is a loose one and that exchange of SDS with the environment occurs at an exceedingly high rate.
Journal ArticleDOI
Improved solubilization procedures for liquid scintillation counting of biological materials
D.L. Hansen,E.T. Bush +1 more
TL;DR: Hyamine hydroxide and NCS appear to be capable of solubilizing soft tissues and many purified biological materials so that low-energy beta emitters (H 3 and C 14 ) can be counted with true solution efficiencies, as determined by internal standardization, channels ratio, etc as mentioned in this paper.
Related Papers (5)
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more