Analysis of platelet-derived extracellular vesicles in plateletpheresis concentrates: a multicenter study.
Anne Black,Evelyn Orsó,Reinhard Kelsch,Melanie Pereira,Julian Kamhieh-Milz,Abdulgabar Salama,Michael B. Fischer,Eduardo Meyer,Beat M. Frey,Gerd Schmitz +9 more
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TLDR
The aim of this multicenter study was to establish and validate a consensus protocol for the standardized PL‐EV quantification using conventional flow cytometers.About:
This article is published in Transfusion.The article was published on 2017-06-01 and is currently open access. It has received 14 citations till now.read more
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Mass spectrometric analysis of lipid species of human circulating blood cells
TL;DR: In this paper, the authors compared lipid species in monocytes, lymphocytes, granulocytes, platelets and red blood cells (RBC) of healthy volunteers using electrospray ionization tandem mass spectrometry and detected striking differences among the examined blood cells.
Journal Article
High-Sensitivity Flow Cytometry Provides Access to Standardized Measurement of Small-Size Microparticles—Brief Report
StéphaneRobert,RomaricLacroix,PhilippePoncelet,KarimHarhouri,TarikBouriche,CoralieJudicone,JenniferWischhusen,LaurentArnaud,FrançoiseDignat-George +8 more
TL;DR: Compared with standard FCM, hs-FCM displayed improved forward scatter resolution and lower background noise, allowing it to discriminate previously undetectable small MP in plasma samples, and to compare MP counts obtained with both sensitivity levels.
Journal ArticleDOI
Dissecting the biochemical architecture and morphological release pathways of the human platelet extracellular vesiculome.
Silvia H. De Paoli,Tseday Z. Tegegn,Oumsalama K. Elhelu,Michael Brad Strader,Mehulkumar Patel,Lukas Diduch,Ivan D. Tarandovskiy,Yong Wu,Jiwen Zheng,Mikhail V Ovanesov,Abdu I. Alayash,Jan Simak +11 more
TL;DR: Four morphological pathways for PEVs release from TRAP-activated PLTs are proposed: plasma membrane budding, extrusion of multivesicular α-granules and cytoplasmic vacuoles, plasma membrane blistering and “pearling” of PLT pseudopodia.
Journal ArticleDOI
Lipid mediators in platelet concentrate and extracellular vesicles: Molecular mechanisms from membrane glycerophospholipids to bioactive molecules
Sami Valkonen,Minna Holopainen,Romain A. Colas,Ulla Impola,Jesmond Dalli,Reijo Käkelä,Pia Siljander,Saara Laitinen +7 more
TL;DR: Since the EVs were enriched in the fatty acid precursors of LMs in their (phospholipid) membranes, harboured LM-producing enzymes, contained the related monohydroxy pathway markers, and secreted the final LM products, PlaC-derived EVs could participate in the regulation of inflammation and healing, and thereby aid the platelets in exerting their essential physiological functions.
Journal ArticleDOI
Complementary roles of platelet αIIbβ3 integrin, phosphatidylserine exposure and cytoskeletal rearrangement in the release of extracellular vesicles.
Alexandra C.A. Heinzmann,Mieke F.A. Karel,Daniëlle Coenen,Tanja Vajen,Nicole Meulendijks,Magdolna Nagy,Dennis Suylen,Judith M.E.M. Cosemans,Johan W. M. Heemskerk,Tilman M. Hackeng,Rory R. Koenen +10 more
TL;DR: Investigation of the role of integrin αIIbβ3, phosphatidyl serine exposure, cytoskeletal rearrangement and their associated signalling pathways in EV release found that platelet EV release requires three main events: integrin activation and closure, PS exposure, and cytoskeleton rearrangements.
References
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Standardization of sample collection, isolation and analysis methods in extracellular vesicle research
Kenneth W. Witwer,Edit I. Buzás,Lynne T. Bemis,Adriana Bora,Cecilia Lässer,Jan Lötvall,Esther Nolte-‘t Hoen,Melissa G. Piper,Sarada Sivaraman,Johan Skog,Clotilde Théry,Marca H. M. Wauben,Fred H. Hochberg +12 more
TL;DR: The need for standardization of specimen handling, appropriate normative controls, and isolation and analysis techniques to facilitate comparison of results is emphasized, and it is recognized that continual development and evaluation of techniques will be necessary as new knowledge is amassed.
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Classification, Functions, and Clinical Relevance of Extracellular Vesicles
TL;DR: Evidence is accumulating that vesicles are cargo containers used by eukaryotic cells to exchange biomolecules as transmembrane receptors and genetic information, and they have a myriad of potential clinical applications, ranging from biomarkers to anticancer therapy.
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Applying extracellular vesicles based therapeutics in clinical trials - an ISEV position paper
Thomas Lener,Mario Gimona,Ludwig Aigner,Verena Börger,Edit I. Buzás,Giovanni Camussi,Nathalie Chaput,Devasis Chatterjee,Felipe A. Court,Hernando A. del Portillo,Lorraine O'Driscoll,Stefano Fais,Juan M. Falcón-Pérez,Ursula Felderhoff-Mueser,Lorenzo Fraile,Yong Song Gho,André Görgens,Ramesh C. Gupta,An Hendrix,Dirk M. Hermann,Andrew F. Hill,Fred H. Hochberg,Peter A. Horn,Dominique P.V. de Kleijn,Lambros Kordelas,Boris W. Kramer,Eva-Maria Krämer-Albers,Sandra Laner-Plamberger,Saara Laitinen,Tommaso Leonardi,Magdalena J. Lorenowicz,Sai Kiang Lim,Jan Lötvall,Casey A. Maguire,Antonio Marcilla,Irina Nazarenko,Takahiro Ochiya,Tushar Patel,Shona Pedersen,Gabriella Pocsfalvi,Stefano Pluchino,Peter J. Quesenberry,Ilona G. Reischl,Francisco J. Rivera,Ralf Sanzenbacher,Katharina Schallmoser,Ineke Slaper-Cortenbach,Dirk Strunk,Torsten Tonn,Pieter Vader,Pieter Vader,Bas W.M. van Balkom,Marca H. M. Wauben,Samir El Andaloussi,Samir El Andaloussi,Clotilde Théry,Eva Rohde,Bernd Giebel +57 more
TL;DR: In this paper, the authors summarize recent developments and the current knowledge of extracellular vesicles (EVs) and discuss safety and regulatory requirements that must be considered for pharmaceutical manufacturing and clinical application.
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Cell-derived microparticles in haemostasis and vascular medicine
TL;DR: The literature regarding microparticles in their widest definition, including ectosomes and exosomes, are reviewed, to focus mainly on their role in haemostasis and vascular medicine.