Journal ArticleDOI
Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin
TLDR
Lactoferricin B was lethal, causing a rapid loss of colony-forming capability in most of the species tested, and Pseudomonas fluorescens, Enterococcus faecalis and Bifidobacterium bifidum strains were highly resistant to this peptide.Abstract:
A physiologically diverse range of Gram-positive and Gram-negative bacteria was found to be susceptible to inhibition and inactivation by lactoferricin B, a peptide produced by gastric pepsin digestion of bovine lactoferrin. The list of susceptible organisms includes Escherichia coli, Salmonella enteritidis, Klebsiella pneumoniae, Proteus vulgaris, Yersinia enterocolitica, Pseudomonas aeruginosa, Campylobacter jejuni, Staphylococcus aureus, Streptococcus mutans, Corynebacterium diphtheriae, Listeria monocytogenes and Clostridium perfringens. Concentrations of lactoferricin B required to cause complete inhibition of growth varied within the range of 0.3 to 150 micrograms/ml, depending on the strain and the culture medium used. The peptide showed activity against E. coli O111 over the range of pH 5.5 to 7.5 and was most effective under slightly alkaline conditions. Its antibacterial effectiveness was reduced in the presence of Na+, K+, Mg2+ or Ca2+ ions, or in the presence of various buffer salts. Lactoferricin B was lethal, causing a rapid loss of colony-forming capability in most of the species tested. Pseudomonas fluorescens, Enterococcus faecalis and Bifidobacterium bifidum strains were highly resistant to this peptide.read more
Citations
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Journal ArticleDOI
Physico-chemical characteristics of goat and sheep milk
TL;DR: In this article, the main forms of caprine and ovine caseino-macropeptides (CMP), which are the soluble C-terminal derivatives from the action of chymosin on β-casein during the milk clotting process of cheesemaking, have been identified and are a good source of antithrombotic peptides.
Journal ArticleDOI
Tryptophan- and arginine-rich antimicrobial peptides: structures and mechanisms of action.
TL;DR: In this review, the structures of a number of different Trp- and Arg-rich antimicrobial peptides are examined and some of the major mechanistic studies are presented.
Journal ArticleDOI
Bioactive Milk Peptides: A Prospectus
TL;DR: This work reviews the scientific literature and attempts to stimulate consideration of the continued use of bioactive peptides and their expanded development as a commercial product.
Journal ArticleDOI
Lactoferrin--a multifunctional protein with antimicrobial properties.
TL;DR: Its extensive antimicrobial activities were originally attributed to its ability to sequester essential iron, however, it is now established that it possesses bactericidal activities as a result of a direct interaction between the protein or lactoferrin-derived peptides.
Journal ArticleDOI
Lactoferrin: a general review
TL;DR: A wide spectrum of functions are ascribed to lactoferrin, which range from a role in the control of iron availability to immune modulation, and more research is necessary to obtain clarity with regard to the exact mechanism of action.
References
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Journal ArticleDOI
Bactericidal activity of human lactoferrin: sensitivity of a variety of microorganisms.
R R Arnold,M Brewer,J J Gauthier +2 more
TL;DR: Variable susceptibilities for a variety of different microorganisms are demonstrated and it appears that there may be a relation between virulence and resistance to lactoferrin.
Journal ArticleDOI
Potent antibacterial peptides generated by pepsin digestion of bovine lactoferrin.
Mamoru Tomita,Wayne Robert Bellamy,Mitsunori Takase,Koji Yamauchi,Hiroyuki Wakabayashi,Kouzou Kawase +5 more
TL;DR: The lactoferrin hydrolysate described in the present study has commercial value as a natural preservative agent for use in foods and cosmetics, and as a functional component of new clinical foods for prevention or treatment of gastrointestinal disease.
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The Intestinal Microflora of Infants: Composition of Fecal Flora in Breast-Fed and Bottle-Fed Infants
TL;DR: The fecal flora of 35 breast‐fed and 35 bottle‐fed babies was determined and the organism that showed the highest number and the highest frequency of occurrence in both groups was Bifidobacterium breve.
Journal ArticleDOI
Defensins: endogenous antibiotic peptides of animal cells.
Journal ArticleDOI
Magainins and the disruption of membrane-linked free-energy transduction
TL;DR: Members of the magainin family report that they dissipate the electric potential across various energy-transducing membranes and thus uncouple respiration from other free-energy-requiring processes, and propose that this is a likely mechanism for the antimicrobial effects of these compounds.