Antimicrobial activity of LysSS, a novel phage endolysin, against Acinetobacter baumannii and Pseudomonas aeruginosa
TLDR
Recombinant LysSS demonstrated that LysSS can be a novel and promising antimicrobial agent against MRSA and MDR Gram-negative bacteria including A. baumannii and P. aeruginosa.Abstract:
Objectives Multidrug-resistant (MDR) bacteria are a major public-health concern. Bacteriophage endolysins (lysins) can be used as novel antimicrobial agents against bacterial infections. In this study, a novel endolysin (LysSS) containing a lysozyme-like domain was evaluated for its antibacterial activity against various species of bacteria. Methods The LysSS-encoding gene was analyzed and cloned and the LysSS recombinant protein was expressed and purified. Purified LysSS was used to determine its antimicrobial activity against various bacterial species in vitro and to measure its protection rate against Acinetobacter baumannii systemic infection in an in vivo murine model. Results Recombinant LysSS showed activity against MDR A. baumannii, MDR Escherichia coli, MDR Klebsiella pneumoniae, MDR Pseudomonas aeruginosa and Salmonella sp. without pre-treatment with an outer membrane permeabiliser. Moreover, LysSS inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration (MIC) of LysSS against 16 MDR A. baumannii strains ranged from 0.063–0.25 mg/mL. LysSS had no cytotoxic effect on A549 human lung cells below 250 μg/mL. In an animal model, mice infected with A. baumannii were protected (40% survival rate with 125 μg LysSS) by intraperitoneal injection of LysSS. Conclusion The current results demonstrate that LysSS may be a novel and promising antimicrobial agent against MRSA and MDR Gram-negative bacteria, including A. baumannii and P. aeruginosa.read more
Citations
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Book ChapterDOI
Therapeutic Efficacy of Bacteriophages
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Acinetobacter Baumannii Phages: Past, Present and Future
Qihang Tu,Mingfang Pu,Yahao Li,Yue Wang,Maochen Li,Lihua Song,Meng Li,Xiaoping An,Huahao Fan,Yigang Tong +9 more
TL;DR: In this article , the authors described the different drug resistances of A. baumannii and some basic properties of phages, analyzed the interaction between phages and their hosts, and focused on A. bayannii phage therapies and discussed the chance and challenge of phage therapy.
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Biological Control of Acinetobacter baumannii: In Vitro and In Vivo Activity, Limitations, and Combination Therapies
TL;DR: A brief overview of the antibiotic resistance and virulence mechanisms associated with A. baumannii is provided and limitations and corresponding mitigations strategies will be outlined, including curtailing resistance development using combination therapies, product stabilisation, and large-scale (up-scaling) production.
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Diverse infective and lytic machineries identified in genome analysis of tailed coliphages against broad spectrum multidrug-resistant Escherichia coli.
Karthika Raveendran,Murugadas Vaiyapuri,Manikantha Benala,Visnuvinayagam Sivam,Madhusudana Rao Badireddy +4 more
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Engineering of lysin by fusion of antimicrobial peptide (cecropin A) enhances its antibacterial properties against multidrug-resistant Acinetobacter baumannii
Md. Maidul Islam,Doo Kim,Kyeong-Yee Kim,Su-Jin Park,Samia Akter,Jeong-Hwa Kim,S. Bang,Shukho Kim,Jungmin Kim,Je Chul Lee,Chang-Won Hong,Minsang Shin +11 more
TL;DR: The engineered AbEndolysin showed synergistic effects with the beta-lactam antibiotics cefotaxime, ceftazidime, and aztreonam, and an additive effect with meropenem and imipenem, which suggest the potential of lysin therapy and may prompt its use as an alternative to antibiotics.
References
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