Antimicrobial activity of LysSS, a novel phage endolysin, against Acinetobacter baumannii and Pseudomonas aeruginosa
TLDR
Recombinant LysSS demonstrated that LysSS can be a novel and promising antimicrobial agent against MRSA and MDR Gram-negative bacteria including A. baumannii and P. aeruginosa.Abstract:
Objectives Multidrug-resistant (MDR) bacteria are a major public-health concern. Bacteriophage endolysins (lysins) can be used as novel antimicrobial agents against bacterial infections. In this study, a novel endolysin (LysSS) containing a lysozyme-like domain was evaluated for its antibacterial activity against various species of bacteria. Methods The LysSS-encoding gene was analyzed and cloned and the LysSS recombinant protein was expressed and purified. Purified LysSS was used to determine its antimicrobial activity against various bacterial species in vitro and to measure its protection rate against Acinetobacter baumannii systemic infection in an in vivo murine model. Results Recombinant LysSS showed activity against MDR A. baumannii, MDR Escherichia coli, MDR Klebsiella pneumoniae, MDR Pseudomonas aeruginosa and Salmonella sp. without pre-treatment with an outer membrane permeabiliser. Moreover, LysSS inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration (MIC) of LysSS against 16 MDR A. baumannii strains ranged from 0.063–0.25 mg/mL. LysSS had no cytotoxic effect on A549 human lung cells below 250 μg/mL. In an animal model, mice infected with A. baumannii were protected (40% survival rate with 125 μg LysSS) by intraperitoneal injection of LysSS. Conclusion The current results demonstrate that LysSS may be a novel and promising antimicrobial agent against MRSA and MDR Gram-negative bacteria, including A. baumannii and P. aeruginosa.read more
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Endolysin, a Promising Solution against Antimicrobial Resistance.
Mujeeb ur Rahman,Weixiao Wang,Qingqing Sun,Junaid Ali Shah,Chao Li,Yanmei Sun,Yuanrui Li,Bailing Zhang,Wei Chen,Shiwei Wang +9 more
TL;DR: This review comprehensively introduces the structures and activities of endolysins and summarize the latest application progress of recombinant endolySins in the fields of medical treatment, pathogen diagnosis, food safety, and agriculture.
Journal ArticleDOI
A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes
Fazal Mehmood Khan,Vijay Singh Gondil,Changchang Li,Mengwei Jiang,Junhua Li,Junping Yu,Hongping Wei,Hang Yang +7 more
TL;DR: In this paper, an A. baumannii bacteriophage p54 was isolated and characterized, and a novel endolysin, namely LysAB54, showing low similarity with other well-known related endolysins, was cloned, expressed, and characterized from the bacteria p54.
Journal ArticleDOI
Non-Antibiotics Strategies to Control Salmonella Infection in Poultry
José Martín Ruvalcaba-Gómez,Zuamí Villagrán,Juan J. Valdez-Alarcón,Marcelino Martínez-Núñez,Lorena Jacqueline Gómez-Godínez,Edmundo Ruesga Gutiérrez,Luis M. Anaya-Esparza,Ramón I. Arteaga-Garibay,Angélica Villarruel-López +8 more
TL;DR: The core aim of this review was exploring the use of complementary strategies, highlighting those based on -omics tools, to assess the effects of using the available antibiotic-free alternatives and their role in lowering dependency on the existing antimicrobial substances to manage bacterial infections in poultry effectively.
Journal ArticleDOI
Novel antimicrobial agents for combating antibiotic-resistant bacteria.
TL;DR: In this article , a review of the promising antimicrobial therapies for the treatment of multidrug-resistant bacterial infections is presented, focusing on preclinical and clinical investigations, as well as combinatorial approaches.
Journal ArticleDOI
Discovering the Potentials of Four Phage Endolysins to Combat Gram-Negative Infections.
Daria V. Vasina,Nataliia P Antonova,Igor V. Grigoriev,Victoria S. Yakimakha,Anastasiya M. Lendel,Maria A. Nikiforova,Andrei A. Pochtovyi,Timofey A. Remizov,Evgeny V Usachev,Natalia V. Shevlyagina,Vladimir G. Zhukhovitsky,Mikhail V. Fursov,Vasiliy D Potapov,Aleksei M. Vorobev,A.V. Aleshkin,Aleksei I. Laishevtsev,Valentine V Makarov,Sergey M Yudin,Artem P. Tkachuk,Vladimir A. Gushchin +19 more
TL;DR: In this paper, the authors provide a comprehensive study of the antimicrobial efficacy aspects of four Gram-negative bacteria-targeting endolysins LysAm24, LysAp22, LysECD7, and LysSi3.
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