Applications of Biomaterials in 3D Cell Culture and Contributions of 3D Cell Culture to Drug Development and Basic Biomedical Research.
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TLDR
A review of biomaterials currently used to improve cellular functions in 3D culture and the contributions of 3D cell culture to cancer research, stem cell culture and drug and toxicity screening can be found in this article.Abstract:
The process of evaluating the efficacy and toxicity of drugs is important in the production of new drugs to treat diseases. Testing in humans is the most accurate method, but there are technical and ethical limitations. To overcome these limitations, various models have been developed in which responses to various external stimuli can be observed to help guide future trials. In particular, three-dimensional (3D) cell culture has a great advantage in simulating the physical and biological functions of tissues in the human body. This article reviews the biomaterials currently used to improve cellular functions in 3D culture and the contributions of 3D culture to cancer research, stem cell culture and drug and toxicity screening.read more
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Hydrogels: Properties and Applications in Biomedicine
Tzu-Chuan Ho,Chin-Chuan Chang,Hung-Pin Chan,Tze Wen Chung,Chih-Wen Shu,Kuo Pin Chuang,Tsai-Hui Duh,Min Yang,Yu-Chang Tyan +8 more
TL;DR: The basic information of hydrogels, such as structure, classification, and synthesis, are introduced and the recent applications ofHydrogels in 3D cell cultures, drug delivery, wound dressing, and tissue engineering are described.
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In Vitro 3D Cultures to Model the Tumor Microenvironment
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Bioadaptive Porous 3D Scaffolds Comprising Cellulose and Chitosan Nanofibers Constructed by Pickering Emulsion Templating
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Precision Medicine Gains Momentum: Novel 3D Models and Stem Cell-Based Approaches in Head and Neck Cancer.
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References
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Journal ArticleDOI
A practical guide to hydrogels for cell culture.
TL;DR: Hydrogels are introduced to those who may be unfamiliar with procedures to culture and study cells with these systems, with a particular focus on commercially available hydrogels.
Journal ArticleDOI
Electrospinning of gelatin fibers and gelatin/PCL composite fibrous scaffolds.
TL;DR: The results suggest the potential of using composite gelatin/PCL fibrous scaffolds for engineering three-dimensional tissues as a promising scaffold for bone-marrow stromal cell culture.
Journal ArticleDOI
Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation
Maria Vinci,Sharon Gowan,F. E. Boxall,Lisa Patterson,Miriam Zimmermann,William Court,Cara Lomas,Marta Mendiola,David Hardisson,Suzanne A. Eccles +9 more
TL;DR: A suite of highly reproducible tumor microplate three-dimensional functional assays are established and validated to enhance the biological relevance of early preclinical cancer studies and increase the translational predictive value of in vitro drug evaluation studies and reduce the need for in vivo studies by more effective triaging of compounds.
Journal ArticleDOI
Biomimetic porous scaffolds for bone tissue engineering
TL;DR: An overview of the design of ideal biomimetic porous scaffolds for bone tissue engineering is presented, and concepts and techniques including the production of a hierarchical structure on both the macro- and nano-scales, the adjustment of biomechanical properties through structural alignment and chemical components, and the control of the biodegradability of the scaffold and its by-products are discussed.
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Small functional groups for controlled differentiation of hydrogel-encapsulated human mesenchymal stem cells.
Danielle S. W. Benoit,Michael P. Schwartz,Michael P. Schwartz,Andrew R. Durney,Kristi S. Anseth,Kristi S. Anseth +5 more
TL;DR: This work demonstrates that encapsulated human mesenchymal stem cells can be induced to differentiate down osteogenic and adipogenic pathways by controlling their three-dimensional environment using tethered small-molecule chemical functional groups tethered to the hydrogel material.