Atomic Structure of Human Adenovirus by Cryo-EM Reveals Interactions Among Protein Networks
Hongrong Liu,Hongrong Liu,Hongrong Liu,Lei Jin,Lei Jin,Sok Boon S. Koh,Ivo Atanasov,Stan Schein,Stan Schein,Lily Wu,Lily Wu,Z. Hong Zhou,Z. Hong Zhou +12 more
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TLDR
The structure of the whole human adenovirus virion is reported at 3.6 angstroms resolution by cryo–electron microscopy (cryo-EM), revealing in situ atomic models of three minor capsid proteins, extensions of the major capsids, and interactions within three protein-protein networks.Abstract:
Construction of a complex virus may involve a hierarchy of assembly elements. Here, we report the structure of the whole human adenovirus virion at 3.6 angstroms resolution by cryo–electron microscopy (cryo-EM), revealing in situ atomic models of three minor capsid proteins (IIIa, VIII, and IX), extensions of the (penton base and hexon) major capsid proteins, and interactions within three protein-protein networks. One network is mediated by protein IIIa at the vertices, within group-of-six (GOS) tiles—a penton base and its five surrounding hexons. Another is mediated by ropes (protein IX) that lash hexons together to form group-of-nine (GON) tiles and bind GONs to GONs. The third, mediated by IIIa and VIII, binds each GOS to five surrounding GONs. Optimization of adenovirus for cancer and gene therapy could target these networks.read more
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RELION: implementation of a Bayesian approach to cryo-EM structure determination.
TL;DR: Developments that reduce the computational costs of the underlying maximum a posteriori (MAP) algorithm, as well as statistical considerations that yield new insights into the accuracy with which the relative orientations of individual particles may be determined are described.
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Understanding and controlling the interaction of nanomaterials with proteins in a physiological environment
Carl Walkey,Warren C. W. Chan +1 more
TL;DR: The formation of the protein corona, its structure and composition, and its influence on the physiological response are discussed, and an 'adsorbome' of 125 plasma proteins that are known to associate with nanomaterials are presented.
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How cryo-EM is revolutionizing structural biology
TL;DR: The recent advances in electron detection and image processing are reviewed and the exciting new opportunities that they offer to structural biology research are illustrated.
Journal ArticleDOI
2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor
Alberto Bartesaghi,Alan Merk,Soojay Banerjee,Doreen Matthies,Xiongwu Wu,Jacqueline L. S. Milne,Sriram Subramaniam +6 more
TL;DR: The structure of a complex between Escherichia coli β-galactosidase and the cell-permeant inhibitor phenylethyl β-d-thiogalactopyranoside is determined by cryo-EM at an average resolution of ~2.2 angstroms, demonstrating that preparation of specimens of adequate quality and intrinsic protein flexibility now represent the major bottlenecks to routinely achieving resolutions close to 2 Å using single-particle cryo
Journal ArticleDOI
Outcome of the First Electron Microscopy Validation Task Force Meeting
Richard Henderson,Andrej Sali,Matthew L. Baker,Bridget Carragher,Batsal Devkota,Kenneth H. Downing,Edward H. Egelman,Zukang Feng,Joachim Frank,Joachim Frank,Nikolaus Grigorieff,Wen Jiang,Steven J. Ludtke,Ohad Medalia,Pawel A. Penczek,Peter B. Rosenthal,Michael G. Rossmann,Michael F. Schmid,Gunnar F. Schröder,Alasdair C. Steven,David L. Stokes,John D. Westbrook,Willy Wriggers,Huanwang Yang,Jasmine Young,Helen M. Berman,Wah Chiu,Gerard J. Kleywegt,Catherine L. Lawson +28 more
TL;DR: This Meeting Review describes the proceedings and conclusions from the inaugural meeting of the Electron Microscopy Validation Validation Task Force organized by the Unified Data Resource for 3DEM and aims to increase the impact of 3DEM in biology and medicine.
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Adenovirus Protein VI Mediates Membrane Disruption following Capsid Disassembly
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