BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing
Po-Han Chen,Chia-I Lee,Yu-Tzu Weng,Woan-Yuh Tarn,Yeou-Ping Tsao,Ping-Chang Kuo,Pang-Hung Hsu,Chu-Wei Huang,Chiun-Sheng Huang,Hsiu-Hsiang Lee,June-Tai Wu,Show-Li Chen +11 more
TLDR
Drosophila and human BCAS2 share a similar function in RNA splicing, which affects cell viability, and overexpression of hBCAS2 rescues these defects.Abstract:
Here, we show that dBCAS2 (CG4980, human Breast Carcinoma Amplified Sequence 2 ortholog) is essential for the viability of Drosophila melanogaster. We find that ubiquitous or tissue-specific depletion of dBCAS2 leads to larval lethality, wing deformities, impaired splicing, and apoptosis. More importantly, overexpression of hBCAS2 rescues these defects. Furthermore, the C-terminal coiled-coil domain of hBCAS2 binds directly to CDC5L and recruits hPrp19/PLRG1 to form a core complex for splicing in mammalian cells and can partially restore wing damage induced by knocking down dBCAS2 in flies. In summary, Drosophila and human BCAS2 share a similar function in RNA splicing, which affects cell viability.read more
Citations
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The PSO4 Protein Complex Associates with Replication Protein A (RPA) and Modulates the Activation of Ataxia Telangiectasia-mutated and Rad3-related (ATR)
TL;DR: The results suggest that the PSO4 complex functionally interacts with RPA and plays an important role in the DNA damage response.
Journal ArticleDOI
BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis.
Wenbo Liu,Fengchao Wang,Qianhua Xu,Junchao Shi,Xiaoxin Zhang,Xukun Lu,Zhen-Ao Zhao,Zheng Gao,Huaixiao Ma,Enkui Duan,Fei Gao,Shaorong Gao,Zhaohong Yi,Lei Li +13 more
TL;DR: The results suggest that BCAS2 regulates alternative splicing in spermatogonia and the transition to meiosis initiation, and male fertility.
Journal ArticleDOI
Unexpected role of the steroid-deficiency protein ecdysoneless in pre-mRNA splicing.
TL;DR: In Drosophila cells, Ecd directly interacts with core components of the U5 snRNP spliceosomal complex, including the conserved Prp8 protein, and is identified as a novel pre-mRNA splicing factor whose function has been conserved in its human counterpart.
Journal ArticleDOI
Exploring the Human-Nipah Virus Protein-Protein Interactome.
TL;DR: This work identified 101 human-Nipah virus protein-protein interactions using an affinity purification approach coupled with mass spectrometry and explored the cellular consequences of some of these interactions, which provide a comprehensive view of the host complexes that are manipulated by viral proteins.
Journal ArticleDOI
BCAS2 is essential for hematopoietic stem and progenitor cell maintenance during zebrafish embryogenesis.
Shanshan Yu,Tao Jiang,Danna Jia,Yunqiao Han,Fei Liu,Yuwen Huang,Zhen Qu,Yuntong Zhao,Jiayi Tu,Yuexia Lv,Jingzhen Li,Xuebin Hu,Zhaojing Lu,Shanshan Han,Yayun Qin,Xiliang Liu,Shanglun Xie,Qing Kenneth Wang,Zhaohui Tang,Daji Luo,Mugen Liu +20 more
TL;DR: It is shown that the bcas2 deletion induces an abnormal alternative splicing of Mdm4 that predisposes cells to undergo p53-mediated apoptosis, which provides a mechanistic explanation of the deficiency observed in HSPCs.
References
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Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches
Mo Chen,James L. Manley +1 more
TL;DR: Great progress has been made by studying individual transcripts and through genome-wide approaches, which together provide a better picture of the mechanistic regulation of alternative pre-mRNA splicing.
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TL;DR: The importance of individual amino acids in maintaining -helical structure (intramolecular interactions) within individual helices, whilst promoting specific coiled-coil interactions (intermolecular interaction) of correct oligomeric state and orientation is outlined.
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CDC25 phosphatases in cancer cells: key players? Good targets?
TL;DR: The roles of CDC25 phosphatases in both normal and abnormal cell proliferation are focused on, a critical assessment of the current data on CDC25 overexpression in cancer is provided, and both current and future therapeutic strategies for targeting CDC25 activity in cancer treatment are discussed.
Journal ArticleDOI
Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.
Gitte Neubauer,Angus King,Juri Rappsilber,Cinzia Calvio,Mark A. Watson,Paul Ajuh,Judith E. Sleeman,Angus I. Lamond,Matthias Mann +8 more
TL;DR: The machinery that removes introns from mRNA precursors — the spliceosome — is a large multi-protein complex that is already sufficiently complete to allow rapid characterization of large mammalian protein complexes via mass spectrometry, and this is the first characterization of an entire mammalian multi- protein complex using these methods.
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Interaction between dorsal and ventral cells in the imaginal disc directs wing development in Drosophila
TL;DR: It is reported here that spatially localized expression of the homeobox gene apterous specifies the identity of dorsal cells in the wing and the boundary of cell lineage restriction between dorsal and ventral compartments coincides with the limit of the domain of ap expression.