BCL-6, a POZ/zinc-finger protein, is a sequence-specific transcriptional repressor
TLDR
It is shown here that BCL-6 is present in DNA-binding complexes in nuclear extracts from various B-cell lines and that two noncontiguous regions, including the POZ motif, mediate maximum transrepressive activity.Abstract:
Approximately 40% of diffuse large cell lymphoma are associated with chromosomal translocations that deregulate the expression of the BCL6 gene by juxtaposing heterologous promoters to the BCL-6 coding domain. The BCL6 gene encodes a 95-kDa protein containing six C-terminal zinc-finger motifs and an N-terminal POZ domain, suggesting that it may function as a transcription factor. By using a DNA sequence selected for its ability to bind recombinant BCL-6 in vitro, we show here that BCL-6 is present in DNA-binding complexes in nuclear extracts from various B-cell lines. In transient transfectin experiments, BCL6 can repress transcription from promoters linked to its DNA target sequence and this activity is dependent upon specific DNA-binding and the presence of an intact N-terminal half of the protein. We demonstrate that this part of the BCL6 molecule contains an autonomous transrepressor domain and that two noncontiguous regions, including the POZ motif, mediate maximum transrepressive activity. These results indicate that the BCL-6 protein can function as a sequence-specific transcriptional repressor and have implications for the role of BCL6 in normal lymphoid development and lymphomagenesis.read more
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References
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Journal ArticleDOI
The POZ domain: a conserved protein-protein interaction motif.
TL;DR: A novel zinc finger protein, ZID, which contains a 120-amino-acid conserved motif present in a large family of proteins that includes both the otherwise unrelated zinc finger proteins and a group of poxvirus proteins, is described.
Journal ArticleDOI
Specific DNA binding of GAL4, a positive regulatory protein of yeast
TL;DR: It is shown that the yeast positive regulatory protein GAL4 binds to four sites in the upstream activating sequence UASG to activate transcription of the adjacent GAL1 and GAL10 genes, consistent with the idea that GAL2 protein binds to three related 17 bp sequences, each of which displays approximate 2-fold rotational symmetry.
Journal ArticleDOI
Alterations of a Zinc Finger-Encoding Gene, BCL-6, in Diffuse Large-Cell Lymphoma
Bihui H. Ye,F. Lista,F. Lo Coco,Daniel M. Knowles,Kenneth Offit,R. S. K. Chaganti,Riccardo Dalla-Favera +6 more
TL;DR: A gene was cloned from chromosomal translocations affecting band 3q27 that codes for a 79-kilodalton protein that is homologous with zinc finger-transcription factors and may be a proto-oncogene specifically involved in the pathogenesis of diffuse large-cell lymphoma.
Journal ArticleDOI
Transcription activation by the adenovirus E1a protein
James W. Lillie,Michael R. Green +1 more
TL;DR: It is shown that Ela has the two functions characteristic of a typical cellular activator: one direct Ela to the promoter, perhaps by interacting with a DMA-bound protein, and the other, an activating region, enables the bound activator to stimulate transcription.
Journal ArticleDOI
BCL-6 protein is expressed in germinal-center B cells
Giorgio Cattoretti,Chih Chao Chang,Katarina Cechova,Jiandong Zhang,Bihui H. Ye,Brunangelo Falini,Diane C. Louie,Kenneth Offit,Raju S.K. Chaganti,Riccardo Dalla-Favera +9 more
TL;DR: The results indicate that the expression of the BCL-6 gene is specifically regulated during B-cell differentiation and suggest a role for B CL-6 in germinal center development or function.