Journal ArticleDOI
Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
TLDR
Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies and the name 'single domain antibodies (dAbs)' is suggested for these antigen binding demands.Abstract:
IN antibodies, a heavy and a light chain variable domain, VH and VL, respectively, pack together and the hypervariable loops on each domain contribute to binding antigen1–4. We find, however, that isolated VH domains with good antigen-binding affinities can also be prepared. Using the polymerase chain reaction5, diverse libraries of VH genes were cloned from the spleen genomic DNA of mice immunized with either lysozyme or keyhole-limpet haemocyanin. From these libraries, VH domains were expressed and secreted from Escherichia coli. Binding activities were detected against both antigens, and two VH domains were characterized with affinities for lysozyme in the 20 nM range. Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies. We suggest the name 'single domain antibodies (dAbs)' for these antigen binding demands.read more
Citations
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Uniform labeling of a recombinant antibody Fv-fragment with 15N and 13C for heteronuclear NMR spectroscopy.
TL;DR: Detailed assignments are possible, but some amino acid specific labeling in addition to uniform labeling will be required for complete assignments of Fv‐fragments.
Patent
ANTI-Ly6E ANTIBODIES AND IMMUNOCONJUGATES AND METHODS OF USE
Peter Chang,Chie Sakanaka +1 more
TL;DR: In this article, the authors provide anti-Ly6E antibodies, immunoconjugates and methods of using the same, as well as methods of applying the same to immunoglobulin.
Journal ArticleDOI
Construction, characterisation and kinetics of a single chain antibody recognising the tumour associated antigen placental alkaline phosphatase
Philip Savage,Gail Rowlinson-Busza,M. Verhoeyen,Robert A. Spooner,A. K. So,J. Windust,P. J. Davis,Agamemnon Antoniou Epenetos +7 more
TL;DR: It is concluded that the H17E2 SCA is suitable for further investigation as an agent for clinical imaging and therapy and can also be used for the construction of antibody based fusion proteins to target other effector functions to tumour cells.
Patent
Human IL-23 Antigen Binding Proteins
Jennifer E. Towne,Cheng Janet D,Jason C. O'neill,Yu Zhang,Yu Sun,Heather Cerne,Derek E. Piper,Randal R. Ketchem +7 more
TL;DR: In this paper, the use of IL-23 antigen binding proteins for diagnostic and therapeutic purposes is discussed. But the authors do not provide a detailed description of the antigen binding protein.
Journal ArticleDOI
Rapid Isolation of Immunoglobulin Variable Genes from Cell Lysates of Rat Hybridomas by Polymerase Chain Reaction
TL;DR: It is shown that the isolation of the rearranged immunoglobulin genes from a hybridoma cell line can easily be achieved by polymerase chain reaction, and that the procedure also works with crude cell lysates as starting material, thereby greatly reducing the time required for sample preparation.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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