Journal ArticleDOI
Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
TLDR
Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies and the name 'single domain antibodies (dAbs)' is suggested for these antigen binding demands.Abstract:
IN antibodies, a heavy and a light chain variable domain, VH and VL, respectively, pack together and the hypervariable loops on each domain contribute to binding antigen1–4. We find, however, that isolated VH domains with good antigen-binding affinities can also be prepared. Using the polymerase chain reaction5, diverse libraries of VH genes were cloned from the spleen genomic DNA of mice immunized with either lysozyme or keyhole-limpet haemocyanin. From these libraries, VH domains were expressed and secreted from Escherichia coli. Binding activities were detected against both antigens, and two VH domains were characterized with affinities for lysozyme in the 20 nM range. Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies. We suggest the name 'single domain antibodies (dAbs)' for these antigen binding demands.read more
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Patent
Method of treating neurodegenerative disease
TL;DR: In this article, an anti-SNCA agent that targets the SNCA gene can have been modified to alter distribution in favor of neural cells, which can be used for the treatment of neurodegenerative disorders.
Journal ArticleDOI
The association−dissociation behavior of the ApoE proteins: kinetic and equilibrium studies.
Kanchan Garai,Carl Frieden +1 more
TL;DR: The approach combining multiple methods establishes the reaction scheme of ApoE self-association as a monomer−dimer−tetramer process, showing that the association−dissociation process is isoform specific.
Patent
Recombinant IL-9 Antibodies And Uses Thereof
TL;DR: In this article, the authors provide novel antibodies that immunospecifically bind to an IL-9 polypeptide and compositions comprising said antibodies, as well as methods and compositions for diagnosing, monitoring, and prognosing these disorders.
Journal ArticleDOI
Single domain antibodies targeting neuraminidase protect against an H5N1 influenza virus challenge
Francisco Miguel Lopez Cardoso,Lorena Itatí Ibañez,Silvie Van den Hoecke,Sarah De Baets,Anouk Smet,Kenny Roose,Bert Schepens,Francis J. Descamps,Walter Fiers,Serge Muyldermans,Ann Depicker,Xavier Saelens +11 more
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Journal ArticleDOI
Monoclonal antibodies: technologies for early discovery and engineering
TL;DR: This review begins with antibody biology and how the structure and genetic machinery relate to function, diversity, and in vivo affinity maturation and follows with the general requirements of (therapeutic) antibody discovery and engineering with an emphasis on in vitro display technologies.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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