Journal ArticleDOI
Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
TLDR
Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies and the name 'single domain antibodies (dAbs)' is suggested for these antigen binding demands.Abstract:
IN antibodies, a heavy and a light chain variable domain, VH and VL, respectively, pack together and the hypervariable loops on each domain contribute to binding antigen1–4. We find, however, that isolated VH domains with good antigen-binding affinities can also be prepared. Using the polymerase chain reaction5, diverse libraries of VH genes were cloned from the spleen genomic DNA of mice immunized with either lysozyme or keyhole-limpet haemocyanin. From these libraries, VH domains were expressed and secreted from Escherichia coli. Binding activities were detected against both antigens, and two VH domains were characterized with affinities for lysozyme in the 20 nM range. Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies. We suggest the name 'single domain antibodies (dAbs)' for these antigen binding demands.read more
Citations
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Kinetic analysis of engineered antibody-antigen interactions.
TL;DR: The detailed analysis both kinetic and equilibrium binding affinity are essential to understand the activity of the molecules resulting from antibody engineering and to guide the progress of their further design.
Patent
Monoclonal antibodies which recognise malignant cells from bladder carcinomas
K. Z. Walker,Pamela J. Russell +1 more
TL;DR: In this article, a family of novel antigens associated with malignant cells of bladder carcinomas is described. And the authors present methods of detecting bladder carcinoma using those antigenectors, fragments or portions thereof, or single domain antibodies, to kits for use in those methods and to methods of treating bladder cancer using the antibodies.
Journal ArticleDOI
A conserved hydrophobic patch on Vβ domains revealed by TCRβ chain crystal structures: Implications for pre-TCR dimerization.
Bo Zhou,Qiang Chen,Robert J. Mallis,Hongmin Zhang,Hongmin Zhang,Jin-huan Liu,Ellis L. Reinherz,Jia-huai Wang +7 more
TL;DR: In this paper, the crystal structures of individual β subunits, termed N15β (Vβ5.2Dβ2Jβ2.6Cβ2), were derived from two αβ TCRs specific for the immunodominant vesicular stomatitis virus octapeptide (VSV-8) bound to the murine H-2K(b) class I molecule.
Patent
Glycan conjugates and use thereof
TL;DR: In this article, a mixture of synthetic glycan conjugates and immunogenic compositions of Neisseria meningitidis is described, where the glycan moiety is covalently linked to the carrier through a linker.
Journal ArticleDOI
"Cleavable" hapten-biotin conjugates: preparation and use for the generation of anti-steroid single-domain antibody fragments.
Norihiro Kobayashi,Hiroyuki Oyama,Masanori Nakano,Tatsuaki Kanda,Erika Banzono,Yoshinori Kato,Tsuyoshi Karibe,Tadashi Nishio,Tadashi Nishio,Junichi Goto +9 more
TL;DR: Cleavable hapten-biotin conjugates are prepared and used to isolate anti-hapten antibody fragments with relatively low affinities and Random point mutations were introduced by error-prone PCR into the gene fragment encoding the V(H) domain of a mouse anti-E(2) antibody.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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