Journal ArticleDOI
Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
TLDR
Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies and the name 'single domain antibodies (dAbs)' is suggested for these antigen binding demands.Abstract:
IN antibodies, a heavy and a light chain variable domain, VH and VL, respectively, pack together and the hypervariable loops on each domain contribute to binding antigen1–4. We find, however, that isolated VH domains with good antigen-binding affinities can also be prepared. Using the polymerase chain reaction5, diverse libraries of VH genes were cloned from the spleen genomic DNA of mice immunized with either lysozyme or keyhole-limpet haemocyanin. From these libraries, VH domains were expressed and secreted from Escherichia coli. Binding activities were detected against both antigens, and two VH domains were characterized with affinities for lysozyme in the 20 nM range. Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies. We suggest the name 'single domain antibodies (dAbs)' for these antigen binding demands.read more
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Patent
Amino acid sequences directed against cxcr4 and other gpcrs and compounds comprising the same
Blanchetot Christophe,Martine Smit,Leurs Regorius,Jahnichen Sven,Michael John Scott Saunders,Haard Johannes Joseph Wilhelmus De,Peter Vanlandschoot +6 more
TL;DR: In this article, the authors reveal that the nanobody polypeptides that specifically bind the human CXCR4 are generated by a method, comprising at least the steps of: 1. immunizing a Llama with whole cells that are alive and express the human-CXCL4 on their surface; 2. selecting for binding for human CCL4; and 3.
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Amino acid sequences directed against her2 and polypeptides comprising the same for the treatment of cancers and/or tumors
TL;DR: The present invention relates to amino acid sequences and Nanobodies that are directed against Epidermal Growth Factor Receptor 2 (HER2), as well as compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acids sequences as discussed by the authors.
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Apoptosis inducing molecule ii and methods of use
TL;DR: In this paper, isolated nucleic acid molecules are provided encoding a human Apoptosis Inducing Molecule II (AIM II), as well as vectors, host cells and recombinant methods for producing the same.
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Plasma kallikrein binding proteins
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Methods and compositions for generating human monoclonal antibodies
TL;DR: In this article, a method for generating human monoclonal antibodies, especially those that are specific for surface antigens representative of a particular cell type, is described. But the method is not suitable for the use of immunoglobulins.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
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Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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