Journal ArticleDOI
Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
TLDR
Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies and the name 'single domain antibodies (dAbs)' is suggested for these antigen binding demands.Abstract:
IN antibodies, a heavy and a light chain variable domain, VH and VL, respectively, pack together and the hypervariable loops on each domain contribute to binding antigen1–4. We find, however, that isolated VH domains with good antigen-binding affinities can also be prepared. Using the polymerase chain reaction5, diverse libraries of VH genes were cloned from the spleen genomic DNA of mice immunized with either lysozyme or keyhole-limpet haemocyanin. From these libraries, VH domains were expressed and secreted from Escherichia coli. Binding activities were detected against both antigens, and two VH domains were characterized with affinities for lysozyme in the 20 nM range. Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies. We suggest the name 'single domain antibodies (dAbs)' for these antigen binding demands.read more
Citations
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Journal ArticleDOI
Primary Structure and Functional scFv Antibody Expression of an Antibody Against the Human Protooncogen c-myc
TL;DR: The immunoglobulin heavy- and light-chain variable region (Vh and Vl) genes were isolated from Myc1-9E10 hybridoma cells, which secreted monoclonal antibody against human oncogen c-myc, to find a genetic heterogeneity for the light chain variable region.
Patent
Methods for identifying modulators of apoptosis
John C. Reed,Bin Guo +1 more
TL;DR: In this article, an isolated polypeptide containing a mitochondrial-derived form of Humanin (SEQ ID NO:3) or a functional fragment thereof where the fragment contains the methionine at position 16 of SEQ ID No:3.
Book ChapterDOI
Expression, purification, and characterization of engineered antibody CH2 and VH domains.
TL;DR: The design, expression, purification, and characterization of engineered CH2 and VH domains are described, which could confer some effector functions on Immunoglobulin-based scaffolds based on a CH2 scaffold.
Patent
Methods and compositions for increasing alpha-l-iduronidase activity in the cns
TL;DR: In this paper, methods and compositions for treating a subject suffering from a deficiency in α-L-Iduronidase in the central nervous system (CNS) are presented.
Patent
Amino acid sequences directed against the angiopoietin/tie system and polypeptides comprising the same for the treatment of diseases and disorders related to angiogenesis
Gonzalez Pajuelo Maria,Michael John Scott Saunders,De Haard Johannes Joseph Wilhelmus,Peter Vanlandschoot +3 more
TL;DR: In this paper, the present invention relates to amino acid sequences that are directed against proteins from the group of the Angiopoietin/Tie family such as Tie 1, Tie 2, Tie 3, Tie 4, Tie 5, Tie 6, Tie 7, Tie 8, Tie 9, Tie 10, Tie 11, Tie 12, Tie 13, Tie 14, Tie 15, Tie 16, Tie 17, Tie 18, Tie 19, Tie 20
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
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Experiments in molecular genetics
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Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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