Journal ArticleDOI
Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
TLDR
Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies and the name 'single domain antibodies (dAbs)' is suggested for these antigen binding demands.Abstract:
IN antibodies, a heavy and a light chain variable domain, VH and VL, respectively, pack together and the hypervariable loops on each domain contribute to binding antigen1–4. We find, however, that isolated VH domains with good antigen-binding affinities can also be prepared. Using the polymerase chain reaction5, diverse libraries of VH genes were cloned from the spleen genomic DNA of mice immunized with either lysozyme or keyhole-limpet haemocyanin. From these libraries, VH domains were expressed and secreted from Escherichia coli. Binding activities were detected against both antigens, and two VH domains were characterized with affinities for lysozyme in the 20 nM range. Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies. We suggest the name 'single domain antibodies (dAbs)' for these antigen binding demands.read more
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Patent
Generation and profiling of fully human hucal gold®-derived therapeutic antibodies specific for human cd38
TL;DR: This paper used recombinant antigen-binding regions and functional fragments to treat hematological malignancies such as multiple myeloma (myeloma) and lymphoma (Lymphoma).
Journal ArticleDOI
Tailor-made antibody therapeutics.
Partha S. Chowdhury,Herren Wu +1 more
TL;DR: The described in this review is an abridged account of the different ways antibodies have been tailored to make them efficient drug molecules.
PatentDOI
Secretion of T cell receptor fragments from recombinant Escherichia coli cells
TL;DR: The secreted TCR fragments can be purified in milligram quantities, and could therefore be used in high-resolution structural studies, in immunization to generate anti-clonotypic antibodies or in vaccination.
Journal ArticleDOI
Development and application of cytotoxic T lymphocyte‐associated antigen 4 as a protein scaffold for the generation of novel binding ligands
Simon E. Hufton,Nicole Coolen van Neer,Twan van den Beuken,Johan Desmet,Erwin Sablon,Hennie R. Hoogenboom +5 more
TL;DR: The data suggest that CTLA‐4 is a suitable human scaffold for engineering single‐domain molecules with one or possibly more binding specificities, and one other potential permissive site diametrically opposite the natural CDR‐like loops was found to be tolerant of peptide insertion.
Journal ArticleDOI
Free immunoglobulin light chain: its biology and implications in diseases.
Takanari Nakano,Masanori Matsui,Ikuo Inoue,Takuya Awata,Shigehiro Katayama,Takayuki Murakoshi +5 more
TL;DR: This review attempts to give a comprehensive view about the biological roles of free IgLC together with the gene expression, secretion, antigen-binding ability, and its metabolic characteristics.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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