Journal ArticleDOI
Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
TLDR
Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies and the name 'single domain antibodies (dAbs)' is suggested for these antigen binding demands.Abstract:
IN antibodies, a heavy and a light chain variable domain, VH and VL, respectively, pack together and the hypervariable loops on each domain contribute to binding antigen1–4. We find, however, that isolated VH domains with good antigen-binding affinities can also be prepared. Using the polymerase chain reaction5, diverse libraries of VH genes were cloned from the spleen genomic DNA of mice immunized with either lysozyme or keyhole-limpet haemocyanin. From these libraries, VH domains were expressed and secreted from Escherichia coli. Binding activities were detected against both antigens, and two VH domains were characterized with affinities for lysozyme in the 20 nM range. Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies. We suggest the name 'single domain antibodies (dAbs)' for these antigen binding demands.read more
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Journal ArticleDOI
Expression in Escherichia coli of soluble and M13 phage-displayed forms of a single-chain antibody fragment specific for digoxin: assessment in a novel drug immunoassay.
Isabelle Navarro-Teulon,Sylvie Peraldi-Roux,Thierry Bernardi,Mariana Marin,Marc Piechaczyk,David Shire,Bernard Pau,Martine Biard-Piechaczyk +7 more
TL;DR: It is reported for the first time that phages displaying scFv may constitute a large source of important new reagents in the field of immunodiagnosis and be directly used to set up a novel type of immunoenzymatic assay for monitoring digoxin in sera of patients treated for either congestive heart failure or cardiac arrythmias.
Journal ArticleDOI
Rabbit single domain antibodies specific to protein C expressed in prokaryotes.
TL;DR: The data indicate the feasibility of generating single domain antibody, specific to protein antigen, from rabbit, and the affinity of the single domain antibodies to the antigen is determined to be 10(6)-10(7) l M-1.
Patent
Neutralizing molecules to influenza viruses
TL;DR: In this paper, the authors present methods and means for identifying, producing, and engineering neutralizing antibodies against influenza A viruses, and to the neutralizing antibody produced by these methods.
Patent
Antibodies against tim3 and uses thereof
Xiao Min Schebye,Mark J. Selby,Michelle Minhua Han,Christine Bee,Andy X. Deng,Anan Chuntharapai,Brigitte Devaux,Huiming Li,Paul O. Sheppard,Alan J. Korman,Daniel F. Ardourel,Ekaterina G. Deyanova,Richard Y.-C. Huang,Guodong Chen,Michelle Kuhne,Hong-An Truong +15 more
TL;DR: In this article, the authors describe the uses of these antibodies, or antigen-binding portions thereof, in therapeutic applications, such as treatment of cancer, and use them in T-cell immunoglobulin and mucin-domain containing-3 (TIM3) protein.
Book ChapterDOI
Label-Free Detection with the Resonant Mirror Biosensor
TL;DR: The resonant mirror (RM) biosensor is a leaky waveguide-based instrument that uses the evanescent field to probe changes in the refractive index at the sensing surface to monitor the interaction between an analyte in solution and its biospecific partner immobilized on the waveguide surface.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
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Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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