Journal ArticleDOI
Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
TLDR
Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies and the name 'single domain antibodies (dAbs)' is suggested for these antigen binding demands.Abstract:
IN antibodies, a heavy and a light chain variable domain, VH and VL, respectively, pack together and the hypervariable loops on each domain contribute to binding antigen1–4. We find, however, that isolated VH domains with good antigen-binding affinities can also be prepared. Using the polymerase chain reaction5, diverse libraries of VH genes were cloned from the spleen genomic DNA of mice immunized with either lysozyme or keyhole-limpet haemocyanin. From these libraries, VH domains were expressed and secreted from Escherichia coli. Binding activities were detected against both antigens, and two VH domains were characterized with affinities for lysozyme in the 20 nM range. Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies. We suggest the name 'single domain antibodies (dAbs)' for these antigen binding demands.read more
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Journal ArticleDOI
Bispecific Antibodies in Cancer Immunotherapy
TL;DR: Bispecific antibodies that recruit immune cells to engage and kill tumor cells are focused on, which will become the next generation of antibody-based therapies to fight cancer.
Book ChapterDOI
From whole monoclonal antibodies to single domain antibodies: think small.
TL;DR: The present chapter will review the major steps of the therapeutic antibodies history and highlight the enormous potential of antibody fragments, either used as multiunits such as bispecific antibodies, single units, or as cell modifiers such as intrabodies or cell surface-expressed molecules.
Journal ArticleDOI
Disulfide linkage engineering for improving biophysical properties of human VH domains.
Dae Young Kim,Hiba Kandalaft,Wen Ding,Shannon Ryan,Henk van Faassen,Tomoko Hirama,Simon J. Foote,Roger MacKenzie,Roger MacKenzie,Jamshid Tanha,Jamshid Tanha,Jamshid Tanha +11 more
TL;DR: The introduced disulfide linkage, formed between Cys54 and Cys78 residues, increased the thermostability of V(H)s by 14-18°C and caused conformational changes, as indicated by reduced binding of the V( H)s to protein A.
Patent
High-throughput nucleotide library sequencing
Francois Vigneault,Briggs Adrian Wrangham,Christopher Clouser,Stephen J. Goldfless,Sonia Timberlake +4 more
TL;DR: In this paper, methods and compositions for immune repertoire sequencing and single cell barcoding are presented, which can be used to pair any two sequences originating from a single cell for antibody and T-cell receptor discovery, disease and immune diagnostics, and low error sequencing.
Patent
Antagonist antibodies directed against calcitonin gene-related peptide and methods using same
Joerg Zeller,Kristian Todd Poulsen,Yasmina Noubia Abdiche,Jaume Pons,Sierra Jones Collier,Arnon Rosenthal +5 more
TL;DR: In this paper, an anti-CGRP antagonist was used to prevent or treat CGRP associated disorders such as vasomotor symptoms such as headache, cluster headache, and tension headache.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
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Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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