Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light
Tina Schrödel,Robert Prevedel,Robert Prevedel,Robert Prevedel,Karin Aumayr,Karin Aumayr,Manuel Zimmer,Alipasha Vaziri,Alipasha Vaziri,Alipasha Vaziri +9 more
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TLDR
A two-photon technique for brain-wide calcium imaging in Caenorhabditis elegans, using wide-field temporal focusing (WF-TeFo) and a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans.Abstract:
Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans, using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans. We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a lab-on-a-chip device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.read more
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Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy
Robert Prevedel,Young-Gyu Yoon,Maximilian Hoffmann,Maximilian Hoffmann,Maximilian Hoffmann,Nikita Pak,Gordon Wetzstein,Saul Kato,Tina Schrödel,Ramesh Raskar,Manuel Zimmer,Edward S. Boyden,Alipasha Vaziri,Alipasha Vaziri,Alipasha Vaziri +14 more
TL;DR: This work demonstrates simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain with high-speed volumetric calcium imaging.
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From the neuron doctrine to neural networks
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Swept confocally-aligned planar excitation (SCAPE) microscopy for high speed volumetric imaging of behaving organisms.
Matthew B. Bouchard,Venkatakaushik Voleti,César S. Mendes,Clay Lacefield,Wesley B. Grueber,Richard S. Mann,Randy M. Bruno,Elizabeth M. C. Hillman +7 more
TL;DR: A new 3D microscopy technique that allows volumetric imaging of living samples at ultra-high speeds: Swept, confocally-aligned planar excitation (SCAPE) microscopy, demonstrated by imaging spontaneous neuronal firing in the intact brain of awake behaving mice, as well as freely moving transgenic Drosophila larvae.
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A guide to light-sheet fluorescence microscopy for multiscale imaging
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Global brain dynamics embed the motor command sequence of Caenorhabditis elegans.
Saul Kato,Harris S. Kaplan,Tina Schrödel,Susanne Skora,Theodore H. Lindsay,Eviatar Yemini,Shawn R. Lockery,Manuel Zimmer +7 more
TL;DR: It is shown that the coordination of neuronal activity patterns into global brain dynamics underlies the high-level organization of behavior and serves as a robust scaffold for action selection in response to sensory input.
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