Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light
Tina Schrödel,Robert Prevedel,Robert Prevedel,Robert Prevedel,Karin Aumayr,Karin Aumayr,Manuel Zimmer,Alipasha Vaziri,Alipasha Vaziri,Alipasha Vaziri +9 more
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TLDR
A two-photon technique for brain-wide calcium imaging in Caenorhabditis elegans, using wide-field temporal focusing (WF-TeFo) and a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans.Abstract:
Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans, using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans. We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a lab-on-a-chip device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.read more
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Neural network features distinguish chemosensory stimuli in Caenorhabditis elegans
TL;DR: This paper used graph-theoretic features of neural network activity to predict ecologically relevant stimulus properties, in particular stimulus identity, in a transparent nematode, Caenorhabditis elegans.
Posted ContentDOI
Automated neuron tracking inside moving and deforming animals using deep learning and targeted augmentation
Core Francisco Park,Mahsa Barzegar Keshteli,Kseniia Korchagina,Ariane Delrocq,Vladislav Susoy,Corinne Jones,Aravinthan D. T. Samuel,Sahand Jamal Rahi +7 more
TL;DR: A method based on a convolutional neural network with specific enhancements which is applied to freely moving Caenorhabditis elegans finds that these neurons show rich behaviors, including switching entrainment on and off dynamically when the animal is exposed to periodic odor pulses.
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Digging deeper: methodologies for high-content phenotyping in Caenorhabditis elegans.
TL;DR: The technological and analytical developments that have enabled deep-phenotyping studies in Caenorhabditis elegans are outlined and how these new technologies and tools are digging into the biological origins of complex, multidimensional phenotypes are highlighted.
Book ChapterDOI
Optically Monitoring and Manipulating Brain and Behavior in C. elegans
TL;DR: Progress on microscopy and instrumentation that allow one to use genetically encoded probes to connect brain and behavior in C. elegans are reviewed.
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Spectrally resolved point-spread-function engineering using a complex medium.
Antoine Boniface,Mickael Mounaix,Baptiste Blochet,Hilton B. de Aguiar,Fabien Quéré,Sylvain Gigan +5 more
TL;DR: In this article, the point spread function of the focused beam both spatially and spectrally, from the measured multi-spectral transmission matrix (MSTM), is demonstrated.
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