Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light
Tina Schrödel,Robert Prevedel,Robert Prevedel,Robert Prevedel,Karin Aumayr,Karin Aumayr,Manuel Zimmer,Alipasha Vaziri,Alipasha Vaziri,Alipasha Vaziri +9 more
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TLDR
A two-photon technique for brain-wide calcium imaging in Caenorhabditis elegans, using wide-field temporal focusing (WF-TeFo) and a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans.Abstract:
Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans, using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans. We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a lab-on-a-chip device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.read more
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Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy
Robert Prevedel,Young-Gyu Yoon,Maximilian Hoffmann,Maximilian Hoffmann,Maximilian Hoffmann,Nikita Pak,Gordon Wetzstein,Saul Kato,Tina Schrödel,Ramesh Raskar,Manuel Zimmer,Edward S. Boyden,Alipasha Vaziri,Alipasha Vaziri,Alipasha Vaziri +14 more
TL;DR: This work demonstrates simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain with high-speed volumetric calcium imaging.
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From the neuron doctrine to neural networks
TL;DR: As a new paradigm for neuroscience, neural network models have the potential to incorporate knowledge acquired with single-neuron approaches to help us understand how emergent functional states generate behaviour, cognition and mental disease.
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Swept confocally-aligned planar excitation (SCAPE) microscopy for high speed volumetric imaging of behaving organisms.
Matthew B. Bouchard,Venkatakaushik Voleti,César S. Mendes,Clay Lacefield,Wesley B. Grueber,Richard S. Mann,Randy M. Bruno,Elizabeth M. C. Hillman +7 more
TL;DR: A new 3D microscopy technique that allows volumetric imaging of living samples at ultra-high speeds: Swept, confocally-aligned planar excitation (SCAPE) microscopy, demonstrated by imaging spontaneous neuronal firing in the intact brain of awake behaving mice, as well as freely moving transgenic Drosophila larvae.
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A guide to light-sheet fluorescence microscopy for multiscale imaging
TL;DR: This work elucidate the key developments and define a simple set of underlying principles governing LSFM, which aim to clarify the decisions to be made for those who wish to develop and use bespoke light-sheet systems and to assist in identifying the best approaches to apply this powerful technique to myriad biological questions.
Journal ArticleDOI
Global brain dynamics embed the motor command sequence of Caenorhabditis elegans.
Saul Kato,Harris S. Kaplan,Tina Schrödel,Susanne Skora,Theodore H. Lindsay,Eviatar Yemini,Shawn R. Lockery,Manuel Zimmer +7 more
TL;DR: It is shown that the coordination of neuronal activity patterns into global brain dynamics underlies the high-level organization of behavior and serves as a robust scaffold for action selection in response to sensory input.
References
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Spatial Asymmetry in the Mechanosensory Phenotypes of the C. elegans DEG/ENaC Gene mec-10
Marios Chatzigeorgiou,Laura J Grundy,Katie S. Kindt,Wei-Hsiang Lee,Monica Driscoll,William R Schafer,William R Schafer +6 more
TL;DR: It is found that mec-10 deletion mutant is important, but not essential, for gentle touch responses in the body touch neurons ALM, PLM, and PVM, and the results suggest that MEC-10 may contribute to only a subset of gentle touch mechanosensory complexes found preferentially at the proximal dendrite.
Journal ArticleDOI
Hyperactivation of B-Type Motor Neurons Results in Aberrant Synchrony of the Caenorhabditis elegans Motor Circuit
Yingchuan Qi,Yingchuan Qi,Michelle D. Po,Michelle D. Po,Patrick Mac,Taizo Kawano,Erik M. Jorgensen,Mei Zhen,Mei Zhen,Yishi Jin +9 more
TL;DR: In vivo calcium imaging shows that expression of a hyperactive, somatodendritic ionotropic acetylcholine receptor ACR-2(gf) in A and B class motor neurons induces aberrant synchronous activity in both ventral- and dorsal-innervating B and A classMotor neurons.