Journal ArticleDOI
Calcium dynamics during trap closure visualized in transgenic Venus flytrap.
Hiraku Suda,Hiraku Suda,Hiroaki Mano,Hiroaki Mano,Hiroaki Mano,Masatsugu Toyota,Masatsugu Toyota,Kenji Fukushima,Kenji Fukushima,Kenji Fukushima,Tetsuro Mimura,Izuo Tsutsui,Rainer Hedrich,Rainer Hedrich,Yosuke Tamada,Yosuke Tamada,Yosuke Tamada,Mitsuyasu Hasebe,Mitsuyasu Hasebe +18 more
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TLDR
The leaves of the carnivorous plant Venus flytrap, Dionaea muscipula (Dionaea) close rapidly to capture insect prey, and the role of Ca2+ in plant movement mechanisms and their evolution is investigated using transgenic Dionaea expressing aCa2+ sensor.Abstract:
The leaves of the carnivorous plant Venus flytrap, Dionaea muscipula (Dionaea) close rapidly to capture insect prey. The closure response usually requires two successive mechanical stimuli to sensory hairs on the leaf blade within approximately 30 s (refs. 1–4). An unknown biological system in Dionaea is thought to memorize the first stimulus and transduce the signal from the sensory hair to the leaf blade2. Here, we link signal memory to calcium dynamics using transgenic Dionaea expressing a Ca2+ sensor. Stimulation of a sensory hair caused an increase in cytosolic Ca2+ concentration ([Ca2+]cyt) starting in the sensory hair and spreading to the leaf blade. A second stimulus increased [Ca2+]cyt to an even higher level, meeting a threshold that is correlated to the leaf blade closure. Because [Ca2+]cyt gradually decreased after the first stimulus, the [Ca2+]cyt increase induced by the second stimulus was insufficient to meet the putative threshold for movement after about 30 s. The Ca2+ wave triggered by mechanical stimulation moved an order of magnitude faster than that induced by wounding in petioles of Arabidopsis thaliana5 and Dionaea. The capacity for rapid movement has evolved repeatedly in flowering plants. This study opens a path to investigate the role of Ca2+ in plant movement mechanisms and their evolution. A transgenic Venus flytrap expressing a fluorescent calcium sensor allows real-time live quantification of calcium waves triggered by sensory hair movement. The study suggests that calcium levels represent the molecular basis for the memory effect that requires two stimulations within 30 s.read more
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Journal ArticleDOI
Organic electrochemical neurons and synapses with ion mediated spiking
Padinhare Cholakkal Harikesh,Chi-Yuan Yang,Deyu Tu,Jennifer Y. Gerasimov,Abdul Manan Dar,Adam Armada‐Moreira,Matteo Massetti,Renee Kroon,David Bliman,Roger Olsson,Eleni Stavrinidou,Magnus Berggren,Simone Fabiano +12 more
TL;DR: The first organic electrochemical neurons (OECNs) with ion-modulated spiking, based on all-printed complementary OEC transistors, were reported in this article .
Journal ArticleDOI
The Venus flytrap trigger hair-specific potassium channel KDM1 can reestablish the K+ gradient required for hapto-electric signaling.
Anda Iosip,Jennifer Böhm,Sönke Scherzer,Khaled A. S. Al-Rasheid,Ingo Dreyer,Jörg Schultz,Dirk Becker,Ines Kreuzer,Rainer Hedrich +8 more
TL;DR: To reveal the molecular basis for the unique functional repertoire of this mechanoresponsive plant structure, the transcriptome of D. muscipula’s trigger hair was determined and the Shaker-type channel KDM1 was electrophysiologically characterized as a hyperpolarization- and acid-activated K+-selective channel.
Journal ArticleDOI
Stretch-activated ion channels identified in the touch-sensitive structures of carnivorous Droseraceae plants.
Carl Procko,Swetha E. Murthy,Swetha E. Murthy,William T Keenan,William T Keenan,Seyed Ali Reza Mousavi,Seyed Ali Reza Mousavi,Tsegaye Dabi,Adam Coombs,Adam Coombs,Erik Procko,Lisa M. Baird,Ardem Patapoutian,Ardem Patapoutian,Joanne Chory +14 more
TL;DR: In this article, the authors used comparative transcriptomics to show that expression of three genes encoding homologs of the MscS-Like (MSL) and OSCA/TMEM63 family of mechanosensitive ion channels are localized to touch-sensitive trigger hairs of the Venus flytrap.
Journal ArticleDOI
Illuminating the hidden world of calcium ions in plants with a universe of indicators
Matteo Grenzi,Francesca Resentini,Steffen Vanneste,Michela Zottini,Andrea Bassi,Andrea Bassi,Alex Costa,Alex Costa +7 more
TL;DR: The tools available to carry out in vivo analysis of Ca2+ dynamics in plants are powerful and mature technologies that still require the proper controls.
Journal ArticleDOI
Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana
Mamoru Matsumura,Mika Nomoto,Tomotaka Itaya,Yuri Aratani,Mizuki Iwamoto,Takakazu Matsuura,Yuki Hayashi,Tsuyoshi Mori,Michael J. Skelly,Yoshiharu Y. Yamamoto,Toshinori Kinoshita,Izumi C. Mori,Takamasa Suzuki,Shigeyuki Betsuyaku,Steven H. Spoel,Masatsugu Toyota,Yasuomi Tada +16 more
TL;DR: In this paper , trichomes, hair-like structures on the epidermis, directly sense external mechanical forces, including raindrops, to anticipate pathogen infections in Arabidopsis thaliana.
References
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Journal ArticleDOI
Ultrasensitive fluorescent proteins for imaging neuronal activity.
Tsai Wen Chen,Trevor J. Wardill,Trevor J. Wardill,Yi Sun,Stefan R. Pulver,Sabine L. Renninger,Amy Baohan,Amy Baohan,Eric R. Schreiter,Rex Kerr,Michael B. Orger,Vivek Jayaraman,Loren L. Looger,Karel Svoboda,Douglas S. Kim +14 more
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Book
Nonlinear Regression Analysis and Its Applications
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TL;DR: This book offers a balanced presentation of the theoretical, practical, and computational aspects of nonlinear regression and provides background material on linear regression, including the geometrical development for linear and nonlinear least squares.
Journal ArticleDOI
Engineered GFP as a vital reporter in plants
TL;DR: It is reported that an extensively modified GFP is a versatile and sensitive reporter in a variety of living plant cells and in transgenic plants, and the codon usage effect might be universal, allowing the design of recombinant proteins with high expression efficiency in evolutionarily distant species such as humans and maize.
Journal ArticleDOI
How the Venus flytrap snaps
TL;DR: This study identifies an ingenious solution to scaling up movements in non-muscular engines and provides a general framework for understanding nastic motion in plants.
Journal ArticleDOI
Technical Focus:a guide to Agrobacterium binary Ti vectors.
TL;DR: This work gratefully acknowledges the support of the Biotechnology and Biological Sciences Research Council for support in the development of pGreen.