Q2. What is the effect of AuNPs@POM on the formation of 1D-M?
Li enrichment area as LNO particles’ junction site was composed of low formation energy, causing the direct growth of 1D-MoS2 NRs.
Q3. What is the main reason for the high stability of the sensor platform?
the sensor platform having high stability was developed owing to negative surface charge thanks to PMo12 polyanions on AuNPs.
Q4. What is the effect of XRD on AuNPs?
In addition, the absence of XRD peaks corresponding to POM means the adsorption of POM on AuNPs surface without agglomeration [45].
Q5. What is the effect of AuNPs on the surface?
AuNPs@POM as sensor platform/surface generally had two aims including the obtainment of the binding sites for primer antibody via amino-gold affinity and the increase of surface conductivity.
Q6. What is the role of molybdenum sulfide in NPs?
Molybdenum sulfide (MoS2) as layered transition metal dichalcogenide facilitates electron transfer owing to its abundant active edge sites [22].
Q7. Why did the increase on electrochemical signals be observed?
Due to AuNPs’ optical properties, a large surface area and electrical conductivity [47,48], the increase on electrochemical signals was observed (curve b).
Q8. Why did AuNPs/GCE perform faster than LNO?
Due to the direct growth of 1D-MoS2 NRs on LNO, 1.0 wt%1D-MoS2 NRs/LNO (curve c) facilitated the electron transfer from LNO to 1D-MoS2 NRs in short distance in comparison with curve b.
Q9. What is the sensitivity of the prepared immunosensor for CA 19-9?
Owing to 1DMoS2 NRs’ active sites and porous LNO’s high surface area, LOD of the prepared immunosensor for CA 19-9 demonstrated a relatively low detection limit in comparison with the other materials/methods.
Q10. What was the reason for the high stability of the CA 19-9 immunosensor?
Another reason for this high stability was the formation of stable hydrogen bonding between hydroxyl groups on H2O and PMo12 polyanions on AuNPs.
Q11. What is the effect of PMo12 polyanions on AuNPs?
The average particle diameters of AuNPs were obtained as about 30–35 nm and the whole AuNPs was well dispersed owing to electrostatic repulsion thanks to PMo12 polyanions on AuNPs.
Q12. What is the spectral pattern of AuNPs@POM composite?
The absorption bands at 1065 cm− 1 and 965 cm− 1 were resulted from P–O and Mo–Od groups of pure polyoxometalate whereas these absorption peaks were observed on longer wavelengths at 1090 cm− 1 and 1022 cm− 1 on FTIR spectrum of AuNPs@POM composite.
Q13. What is the hapten of human blood group Lewis antigen?
This antigen has a carbohydrate structure as a sialyl derivative of Lacto-N-fucopentaose II, which is the hapten of human blood group Lewis antigen.
Q14. What was the effect of the preparation of 1.0 wt% 1D-Mo?
after the preparation of 1.0 wt% 1D-MoS2 NS/LNO, no evident MoS2 phase was obtained for 1.0 wt%1DMoS2 NS/LNO such as 1.0 wt%1D-MoS2 NRs/LNO with small MoS2 loading.
Q15. How was the synthesis of AuNPs at POM performed?
The ultrasonic synthesis method was applied to the preparation of AuNPs@POM nanocomposite with mol ratio of AuNPs:POM (1:1) during 45 min.
Q16. What was the calibration equation of the prepared immunosensor for CA 19-9?
In addition, the standard addition method (SAM) was applied the plasma samples and the calibration equation of SAM was obtained as y (I, µA) = 0.4794x (CA 19-9 concentration, µUM.L. Yola and N. AtarMicrochemical Journal 170 (2021) 106643mL−
Q17. What was the effect of the immobilizations of AuNPs on the electrochemical sensor?
As in the CV experiments, when primer antibody (curve d), BSA (curve e) and CA 19-9 (curve f) were immobilized to the sensor platform, the electron transfer rate decreased.
Q18. What is the effect of the immobilization of AuNPs on the electrochemical immunosensor?
as expected, the highest electrochemical currents were observed by using CA 19-9 electrochemical immunosensor based on 2.5 wt%1D-MoS2 NRs/LNO.
Q19. How many wt%1D-MoS2 NS powders were prepared?
After 100.0 µU mL− 1 anti-CA 19-9-Ab2 dispersion (30.0 μL) was prepared in 0.1 M PBS (pH 7.0), this dispersion was interacted with 1DMoS2 NRs/LNO (30.0 μL, 50.0 mg mL− 1) dispersion under magnetic stirring at 37.0 ◦C for 25 min.