Journal ArticleDOI
Characterization in vitro and engraftment potential in vivo of human progenitor T cells generated from hematopoietic stem cells
Geneve Awong,Elaine Herer,Charles D. Surh,John E. Dick,Ross La Motte-Mohs,Juan Carlos Zúñiga-Pflücker +5 more
TLDR
The identification of T-progenitor subsets readily generated in vitro may offer important avenues to improve cellular-based immune-reconstitution approaches.About:
This article is published in Blood.The article was published on 2009-07-30. It has received 137 citations till now. The article focuses on the topics: Progenitor cell & Stem cell.read more
Citations
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Journal ArticleDOI
Coexistence of LMPP-like and GMP-like Leukemia Stem Cells in Acute Myeloid Leukemia
Nicolas Goardon,Emanuele Marchi,Ann Atzberger,Lynn Quek,Anna Schuh,Shamit Soneji,Petter S. Woll,Adam J. Mead,Kate A. Alford,R Rout,Salma Chaudhury,Amanda F. Gilkes,Steven Knapper,Kheira Beldjord,Suriya Begum,Susan Rose,Nicola Geddes,Mike Griffiths,Graham R. Standen,Alexander Sternberg,Jamie Cavenagh,Hannah Hunter,David G. Bowen,Sally Killick,L. G. Robinson,Andrew Price,Elizabeth Macintyre,Paul Virgo,Alan Kenneth Burnett,Charles Craddock,Tariq Enver,Sten Eirik W. Jacobsen,Catherine Porcher,Paresh Vyas +33 more
TL;DR: It is shown that in ∼80% of primary human CD34+ acute myeloid leukemia (AML), two expanded populations with hemopoietic progenitor immunophenotype coexist in most patients, suggesting that in most cases primaryCD34+ AML is a progenitors disease where LSCs acquire abnormal self-renewal potential.
Journal ArticleDOI
T Lymphocyte Potential Marks the Emergence of Definitive Hematopoietic Progenitors in Human Pluripotent Stem Cell Differentiation Cultures
Marion Kennedy,Geneve Awong,Geneve Awong,Christopher M. Sturgeon,Andrea Ditadi,Ross LaMotte-Mohs,Juan Carlos Zúñiga-Pflücker,Gordon Keller +7 more
TL;DR: This study uses T lymphocyte potential to track the onset of definitive hematopoiesis from human embryonic and induced pluripotent stem cells differentiated with specific morphogens in serum- and stromal-free cultures, and shows that this program develops from a progenitor population with characteristics of hemogenic endothelium.
Journal ArticleDOI
Natural killer cell-triggered vascular transformation: maternal care before birth?
TL;DR: Key information concerning uNK cell functions from studies in rodents is reviewed, highlights concerning human uNK cells are summarized, and preliminary studies on development of a humanized, pregnant mouse model for in vivo investigations of human u NK cell functions are described.
Journal ArticleDOI
Enabling stem cell therapies through synthetic stem cell-niche engineering.
Raheem Peerani,Peter W. Zandstra +1 more
TL;DR: Using the HSC niche as a model, microscale engineering strategies capable of systematically examining and reconstructing individual niche components are discussed, suggesting synthetic stem cell-niche engineering may form a new foundation for regenerative therapies.
Journal ArticleDOI
Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids.
Christopher S. Seet,Chongbin He,Michael T. Bethune,Suwen Li,Brent Chick,Eric H. Gschweng,Yuhua Zhu,Kenneth Kim,Donald B. Kohn,David Baltimore,Amélie Montel-Hagen +10 more
TL;DR: A serum-free, artificial thymic organoid (ATO) system that supports efficient and reproducible in vitro differentiation and positive selection of conventional human T cells from all sources of HSPCs is described.
References
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Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells.
Leonard D. Shultz,Bonnie L. Lyons,Lisa M. Burzenski,Bruce Gott,Xiaohua Chen,Stanley Chaleff,Malak Kotb,Stephen D. Gillies,Marie King,Julie A. Mangada,Dale L. Greiner,Rupert Handgretinger +11 more
TL;DR: NOD-scid IL2Rγnull mice engrafted with human mobilized blood stem cells provide a new in vivo long-lived model of robust multilineage human HSC engraftment.
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NOD/SCID/gamma(c)(null) mouse: an excellent recipient mouse model for engraftment of human cells.
Mamoru Ito,Hidefumi Hiramatsu,Kimio Kobayashi,Kazutomo Suzue,Mariko Kawahata,Kyoji Hioki,Yoshito Ueyama,Yoshio Koyanagi,Kazuo Sugamura,Kohichiro Tsuji,Toshio Heike,Tatsutoshi Nakahata +11 more
TL;DR: It is suggested that multiple immunological dysfunctions, including cytokine production capability, in addition to functional incompetence of T, B, and NK cells, may lead to the high engraftment levels of xenograft in NOD/SCID/gamma(c)(null) mice.
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Induction of T Cell Development from Hematopoietic Progenitor Cells by Delta-like-1 In Vitro
TL;DR: It is established that expression of Delta-like-1 on stromal cells provides key signals for the induction of T cell lineage commitment, stage-specific progenitor expansion, TCR gene rearrangement, and T cell differentiation in the absence of a thymus.
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Development of a human adaptive immune system in cord blood cell-transplanted mice.
Elisabetta Traggiai,Laurie Chicha,Luca Mazzucchelli,Lucio Bronz,Jean-Claude Piffaretti,Antonio Lanzavecchia,Markus G. Manz +6 more
TL;DR: It is shown that intrahepatic injection of CD34+ human cord blood cells into conditioned newborn Rag2-/–γc–/– mice leads to de novo development of B, T, and dendritic cells; formation of structured primary and secondary lymphoid organs; and production of functional immune responses.
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Notch regulation of lymphocyte development and function
TL;DR: The function of Notch signaling during lymphopoiesis and T cell neoplasia, based on gain-of-function and conditional loss- of-function approaches for the Notch1 receptor, indicates notch1 is essential in T cell lineage commitment.