Characterization of serum microRNAs profile of PCOS and identification of novel non-invasive biomarkers.
TLDR
Serum miRNAs are differentially expressed between PCOS patients and controls, and bioinformatics analysis indicated that the predicted targets function of the three miRN as mainly involved in the metastasis, cell cycle, apoptosis and endocrine.Abstract:
Background: Polycystic ovary syndrome (PCOS), the most common endocrinopathy in women of reproductive age, is characterized by polycystic ovaries, chronic anovulation, hyperandrogenism and insulin resistance. Despite the high prevalence of hyperandrogenemia, a definitive endocrine marker for PCOS has so far not been identified. Circulating miRNAs have recently been shown to serve as diagnostic/prognostic biomarkers in patients with cancers. Our current study focused on the altered expression of serum miRNAs and their correlation with PCOS. Method and Results: We systematically used the TaqMan Low Density Array followed by individual quantitative reverse transcription polymerase chain reaction assays to identify and validate the expression of serum miRNAs of PCOS patients. The expression levels of three miRNAs (miR-222, miR-146a and miR-30c) were significantly increased in PCOS patients with respect to the controls in our discovery evaluation and followed validation. The area under the receiver operating characteristic (ROC) curve (AUC) is 0.799, 0.706, and 0.688, respectively. The combination of the three miRNAs using multiple logistic regression analysis showed a larger AUC (0.852) that was more efficient for the diagnosis of PCOS. In addition, logistic binary regression analyses show miR-222 is positively associated with serum insulin, while miR-146a is negatively associated with serum testosterone. Furthermore, bioinformatics analysis indicated that the predicted targets function of the three miRNAs mainly involved in the metastasis, cell cycle, apoptosis and endocrine. Conclusion: Serum miRNAs are differentially expressed between PCOS patients and controls. We identified and validated a class of three serum miRNAs that could act as novel non-invasive biomarkers for diagnosis of PCOS. These miRNAs may be involved in the pathogenesis of PCOS.read more
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The Versatile Role of microRNA-30a in Human Cancer
TL;DR: Recent research regarding miR-30a is summarized, including its biological function, expression and regulation, especially focusing on its role in cancer development and progression, which may serve as a potential target in the diagnosis and therapy of human cancer.
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MicroRNAs Related to Polycystic Ovary Syndrome (PCOS)
Anja Elaine Sørensen,Marie Louise Muff Wissing,Sofia Salö,Anne Lis Mikkelsen Englund,Louise Torp Dalgaard +4 more
TL;DR: The understanding of miRNAs, particularly in relation to PCOS, is currently at a very early stage, and additional studies will yield important insight into the molecular mechanisms behind this complex and heterogenic syndrome.
Journal ArticleDOI
Characterization of microRNA profile in human cumulus granulosa cells: Identification of microRNAs that regulate Notch signaling and are associated with PCOS
TL;DR: The data suggested that miRNAs and their targeted pathways play important roles in the etiology and pathophysiology of PCOS, and provides novel candidates for molecular biomarkers or treatment targets in the research of female infertility associated with PCOS.
Journal ArticleDOI
Circulating microRNAs as biomarkers in cancer diagnosis
TL;DR: This review will provide an insight into the extracellular miRNAs, particularly, their release mechanisms and characteristics, and the potential of ext racellular mi RNAs as a biomarker in cancer detection.
Journal ArticleDOI
The role of MiRNA in polycystic ovary syndrome (PCOS).
TL;DR: This paper will review the recent research on miRNAs with respect to PCOS and shed light on new strategies for the diagnosis and treatment of this syndrome.
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