Comparison of mRNA and protein measures of cytokines following vaccination with human papillomavirus-16 L1 virus-like particles
Fatma M. Shebl,Ligia A. Pinto,Alfonso J. García-Piñeres,Alfonso J. García-Piñeres,Richard A. Lempicki,Marcus Williams,Clayton Harro,Allan Hildesheim +7 more
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TLDR
The degree of correlation between gene expression and protein levels varied among different cytokines/chemokines, and researchers should be cautious when using mRNA expression array results as a proxy for protein levels using existing technologies.Abstract:
Background: mRNA expression signatures are frequently used as surrogate measures of cellular function and pathway changes. Few studies have directly compared results obtained using gene expression and multiplex protein assays for corresponding gene products.
Methods: We used data available from a clinical trial of a human papillomavirus-16 vaccine that tracked gene expression and cytokine/chemokine production by peripheral blood mononuclear cells stimulated in culture with various antigens to evaluate the degree to which gene expression levels reflect observed levels of cytokines/chemokines. Twenty-six women enrolled in a phase II clinical trial of a human papillomavirus-16 vaccine were evaluated for gene expression (using the Affymetrix Human Genome Focus Array) and cytokine/chemokine levels (using a bead-based 22-plex cytokine assay developed by Linco Research, Inc.) before and after vaccination.
Results: Our results suggest the presence of a wide range of correlations between mRNA expression and secreted protein levels. The strongest correlation was observed for IFN-γ ( R = 0.90 overall levels; R = 0.69 when vaccine induced changes were evaluated). More modest overall correlations ranging from 0.40 to 0.80 were observed for MIP1A, IP10, TNF-α, MCP1, IL-2, GM-CSF, IL-5, RANTES, and IL-8. Weaker or no correlation was observed between gene expression and protein levels for the remaining cytokines/chemokines evaluated.
Conclusion: The degree of correlation between gene expression and protein levels varied among different cytokines/chemokines.
Impact: Researchers should be cautious when using mRNA expression array results as a proxy for protein levels using existing technologies. Cancer Epidemiol Biomarkers Prev; 19(4); 978–81. ©2010 AACR.read more
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References
More filters
Journal ArticleDOI
A comparison of selected mRNA and protein abundances in human liver
Leigh Anderson,Jeff Seilhamer +1 more
TL;DR: A comparison with published data on the abundances of liver mRNAs for plasma proteins (secreted by the liver) suggests that higher abundance messages are strongly enriched in secreted sequences, and the data confirms this.
Journal ArticleDOI
Global signatures of protein and mRNA expression levels
TL;DR: This review summarizes the state of knowledge about large-scale measurements of absolute protein and mRNA expression levels, and the degree of correlation between the two parameters.
Journal ArticleDOI
Discordant Protein and mRNA Expression in Lung Adenocarcinomas
Guoan Chen,Tarek G. Gharib,Chiang Ching Huang,Jeremy M. G. Taylor,David E. Misek,Sharon L.R. Kardia,Thomas J. Giordano,Mark D. Iannettoni,Mark B. Orringer,Samir M. Hanash,David G. Beer +10 more
TL;DR: The relationship between gene expression measured at the mRNA level and the corresponding protein level is not well characterized in human cancer, and it is shown that only a subset of the proteins exhibited a significant correlation with mRNA abundance.
Journal ArticleDOI
Integrated Genomic and Proteomic Analyses of Gene Expression in Mammalian Cells
Qiang Tian,Serguei B. Stepaniants,Mao Mao,Lee Weng,Megan C. Feetham,Michelle J. Doyle,Eugene C. Yi,Hongyue Dai,Vesteinn Thorsson,Jimmy K. Eng,David R. Goodlett,Joel P. Berger,Bert Gunter,Peter S. Linseley,Roland Stoughton,Ruedi Aebersold,Steven J. Collins,William A. Hanlon,Leroy Hood +18 more
TL;DR: Although the overall pattern of protein expression is similar to that of mRNA expression, the incongruent expression between mRNAs and proteins emphasize the importance of posttranscriptional regulatory mechanisms in cellular development or perturbation that can be unveiled only through integrated analyses of both proteins and m RNAs.
Journal ArticleDOI
Evaluation of gene expression measurements from commercial microarray platforms
Paul K. Tan,Thomas J. Downey,Edward L. Spitznagel,Pin Xu,Dadin Fu,Dimiter S. Dimitrov,Richard A. Lempicki,Bruce M. Raaka,Margaret C. Cam +8 more
TL;DR: Correlations in gene expression levels and comparisons for significant gene expression changes in this subset were calculated, and showed considerable divergence across the different platforms, suggesting the need for establishing industrial manufacturing standards, and further independent and thorough validation of the technology.