Complete Sequences of Two Plasmids in a blaNDM-1-Positive Klebsiella oxytoca Isolate from Taiwan
Tzu Wen Huang,Jann-Tay Wang,Tsai Ling Lauderdale,Tsai Lien Liao,Jui Fen Lai,Mei Chen Tan,Ann Chi Lin,Ying-Tsong Chen,Ying-Tsong Chen,Shih-Feng Tsai,Shan-Chwen Chang +10 more
Reads0
Chats0
TLDR
Findings highlight the difficulty in treating multidrug-resistant bacterial infections and the potential danger of emerging resistant enterobacteria.Abstract:
Genetic determinants of a bla NDM-1 -positive, multidrug-resistant bacterial isolate that caused active infection was investigated by DNA sequencing. Two plasmids, pKOX_NDM1 and pKOX-R1, were identified for the Klebsiella oxytoca strain E718. Sequence annotation revealed a bla NDM-1 gene in pKOX_NDM1 and two extended-spectrum β-lactamase producers ( bla CTX-M-3 and bla SHV-12 ) and a wide array of resistance genes in pKOX-R1. These findings highlight the difficulty in treating multidrug-resistant bacterial infections and the potential danger of emerging resistant enterobacteria.read more
Citations
More filters
Journal ArticleDOI
NDM Metallo-β-Lactamases and Their Bacterial Producers in Health Care Settings.
TL;DR: New Delhi metallo-β-lactamase will remain a severe challenge in health care settings, and more studies on appropriate countermeasures are required, as commonly used phenotypic tests cannot specifically identify NDM.
Journal ArticleDOI
Carbapenemases: Transforming Acinetobacter baumannii into a Yet More Dangerous Menace.
TL;DR: The enzymes within the OXA, NDM, VIM, IMP, and KPC groups of carbapenemases and the coding genes found in A. baumannii clinical isolates are described.
Journal ArticleDOI
Carbapenem-Nonsusceptible Enterobacteriaceae in Taiwan
Jann-Tay Wang,Un-In Wu,Tsai-Ling Lauderdale,Mei-Chen Chen,Shu-Ying Li,Le-Yin Hsu,Shan-Chwen Chang +6 more
TL;DR: Although the prevalence of CPE is still low, careful monitoring is urgently needed, as intra- and inter-hospital spread of KPC-2-producing K. pneumoniae and IMP-8-producing E. cloacae likely occurred.
Journal ArticleDOI
KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan
Ying-Tsong Chen,Ying-Tsong Chen,Jung-Chung Lin,Chang-Phone Fung,Po-Liang Lu,Yin Ching Chuang,Tsu-Lan Wu,L. Kristopher Siu,L. Kristopher Siu,L. Kristopher Siu +9 more
TL;DR: Differences in cross-species transferability of the two plasmids can be explained by genetic differences between their backbones and could have resulted in the confined bla(KPC-2)-carrying CR-KP outbreak in Taiwan.
Journal ArticleDOI
Genomic Characteristics of NDM-Producing Enterobacteriaceae Isolates in Australia and Their blaNDM Genetic Contexts
Alexander M. Wailan,David L. Paterson,Karina Kennedy,Paul R. Ingram,Evan Bursle,Hanna E. Sidjabat +5 more
TL;DR: Genomically characterized 12 NDM-producing Enterobacteriaceae isolated in Australia between 2012 and 2014 and identified five particular plasmid types with specific blaNDM genetic contexts, three of which were IncFII plasmids (FII-A to -C) and P. rettgeri was not conjugative.
References
More filters
Journal ArticleDOI
Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study
Karthikeyan Kumarasamy,Mark Toleman,Timothy R. Walsh,Jay Bagaria,Fafhana Butt,Ravikumar Balakrishnan,Uma Chaudhary,Michel Doumith,Christian G. Giske,Seema Irfan,Padma Krishnan,Anil Kumar,Sunil Maharjan,Shazad Mushtaq,Tabassum Noorie,David L. Paterson,A. Pearson,Claire Perry,Rachel Pike,Bhargavi Rao,Ujjwayini Ray,Jayanta B. Sarma,Madhu Sharma,Elizabeth Sheridan,M A Thirunarayan,Jane F. Turton,Supriya Upadhyay,Marina Warner,William Welfare,David M. Livermore,Neil Woodford +30 more
TL;DR: The prevalence of NDM-1, in multidrug-resistant Enterobacteriaceae in India, Pakistan, and the UK is investigated, and co-ordinated international surveillance is needed.
Journal ArticleDOI
Characterization of a New Metallo-β-Lactamase Gene, blaNDM-1, and a Novel Erythromycin Esterase Gene Carried on a Unique Genetic Structure in Klebsiella pneumoniae Sequence Type 14 from India
Dongeun Yong,Mark Toleman,Christian G. Giske,Hyun Sun Cho,Kristina Sundman,Kyungwon Lee,Timothy R. Walsh +6 more
TL;DR: A Swedish patient of Indian origin traveled to New Delhi, India, and acquired a urinary tract infection caused by a carbapenem-resistant Klebsiella pneumoniae strain that typed to the sequence type 14 complex, showing broad resistance carried on these plasmids.
Journal ArticleDOI
ISfinder: the reference centre for bacterial insertion sequences.
TL;DR: This database is currently recommended by several microbiology journals for registration of new IS elements before their publication and is involved in annotating the IS content of these genomes.
Journal ArticleDOI
The CGView Server: a comparative genomics tool for circular genomes
Jason R. Grant,Paul Stothard +1 more
TL;DR: The CGView Server generates graphical maps of circular genomes that show sequence features, base composition plots, analysis results and sequence similarity plots, and can aid in the identification of conserved genome segments, instances of horizontal gene transfer, and differences in gene copy number.
Journal ArticleDOI
Genetic Features of blaNDM-1-Positive Enterobacteriaceae
TL;DR: The blaNDM-1 gene was carried by different plasmid types (IncA/C, IncF, IncL/M, or untypeable) and was likely chromosome borne in two isolates and coharbored a variety of resistance determinants, including β-lactamase genes, quinolone resistance genes, and 16S RNA methylase genes.
Related Papers (5)
Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study
Karthikeyan Kumarasamy,Mark Toleman,Timothy R. Walsh,Jay Bagaria,Fafhana Butt,Ravikumar Balakrishnan,Uma Chaudhary,Michel Doumith,Christian G. Giske,Seema Irfan,Padma Krishnan,Anil Kumar,Sunil Maharjan,Shazad Mushtaq,Tabassum Noorie,David L. Paterson,A. Pearson,Claire Perry,Rachel Pike,Bhargavi Rao,Ujjwayini Ray,Jayanta B. Sarma,Madhu Sharma,Elizabeth Sheridan,M A Thirunarayan,Jane F. Turton,Supriya Upadhyay,Marina Warner,William Welfare,David M. Livermore,Neil Woodford +30 more
The RAST Server: Rapid Annotations using Subsystems Technology
Ramy K. Aziz,Ramy K. Aziz,Daniela Bartels,Aaron A. Best,Matthew DeJongh,Terrence Disz,Terrence Disz,Robert Edwards,Kevin Formsma,Svetlana Gerdes,Elizabeth M. Glass,Michael Kubal,Folker Meyer,Folker Meyer,Gary J. Olsen,Gary J. Olsen,Robert Olson,Robert Olson,Andrei L. Osterman,Ross Overbeek,Leslie Klis McNeil,Daniel Paarmann,Tobias Paczian,Bruce Parrello,Gordon D. Pusch,Claudia I. Reich,Rick Stevens,Rick Stevens,Olga Vassieva,Veronika Vonstein,Andreas Wilke,Olga Zagnitko +31 more