Correlative Light and Volume Electron Microscopy (vCLEM): How Community Participation Can Advance Developing Technologies
TLDR
Correlative light and electron microscopy is a valuable tool to image samples across resolution scales and link data on structure and function as discussed by the authors, and recent developments have enabled applying these workflows to large volumes of cells and tissues.Abstract:
Correlative light and electron microscopy is a valuable tool to image samples across resolution scales and link data on structure and function. While studies using this technique have been available since the 1960s, recent developments have enabled applying these workflows to large volumes of cells and tissues. Much of the development in this area has been facilitated through the collaborative efforts of microscopists and commercial companies to bring the methods, hardware and image processing technologies needed into laboratories and core imaging facilities. This is a prime example of how what was once a niche area can be brought into the mainstream of microscopy by the efforts of imaging pioneers who push the boundaries of possibility.read more
Citations
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Volume electron microscopy
Christopher J. Peddie,Christel Genoud,Anna Kreshuk,Kimberly Meechan,Kristina D. Micheva,Kedar Narayan,Constantin Pape,Robert G. Parton,Nicole L. Schieber,Yannick Schwab,B. Titze,Paul Verkade,Aubrey V. Weigel,Lucy M. Collinson +13 more
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Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale
TL;DR: This review of the main light and electron microscopy techniques used to unravel the structure and composition of the three cell-cell contacts in epithelial and endothelial cells functions as a guide to pick the appropriate imaging technique(s) for the adhesion complexes of interest.
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Correlative light and volume electron microscopy to study brain development.
TL;DR: In this article , the authors discuss potential applications of genetic manipulation of target cells using clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) and subsequent volume EM to analysis of protein localisation as well as to loss of function studies of genes regulating brain development.
References
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Microscopy Image Browser: A Platform for Segmentation and Analysis of Multidimensional Datasets.
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Journal ArticleDOI
eC-CLEM: flexible multidimensional registration software for correlative microscopies
Perrine Paul-Gilloteaux,Xavier Heiligenstein,Martin Belle,Marie-Charlotte Domart,Banafshé Larijani,Lucy M. Collinson,Graça Raposo,Jean Salamero +7 more
TL;DR: This research presents a novel probabilistic procedure that allows for direct measurement of the response of the immune system to carbon dioxide in the presence of carbon monoxide.
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