COVID-19 serology in nephrology healthcare workers.
Thomas Reiter,Sahra Pajenda,Ludwig Wagner,Martina Gaggl,Johanna Atamaniuk,Barbara Holzer,Irene Zimpernik,Daniela Gerges,Katharina A. Mayer,Christof Aigner,Robert Straßl,Sonja Jansen-Skoupy,Manuela Födinger,Gere Sunder-Plassmann,Alice Schmidt +14 more
TLDR
In this article, the seroprevalence of SARS-CoV-2 IgG and IgM antibodies among healthcare workers of a tertiary care kidney center during the first peak phase of the corona virus disease 2019 (COVID-19) crisis in Austria using an orthogonal test strategy and a total of 12 commercial nucleocapsid protein or spike glycoprotein-based assays as well as Western blotting and a neutralization assay.Abstract:
Chronic kidney disease patients show a high mortality in cases of a severe acute respiratory syndrome coronavirus-2 (SARS-CoV‑2) infection. Thus, information on the sero-status of nephrology personnel might be crucial for patient protection; however, limited information exists about the presence of SARS-CoV‑2 antibodies in asymptomatic individuals. We examined the seroprevalence of SARS-CoV‑2 IgG and IgM antibodies among healthcare workers of a tertiary care kidney center during the the first peak phase of the corona virus disease 2019 (COVID-19) crisis in Austria using an orthogonal test strategy and a total of 12 commercial nucleocapsid protein or spike glycoprotein-based assays as well as Western blotting and a neutralization assay. At baseline 60 of 235 study participants (25.5%, 95% confidence interval, CI 20.4–31.5%) were judged to be borderline positive or positive for IgM or IgG using a high sensitivity/low specificity threshold in one test system. Follow-up analysis after about 2 weeks revealed IgG positivity in 12 (5.1%, 95% CI: 2.9–8.8%) and IgM positivity in 6 (2.6%, 95% CI: 1.1–5.6) in at least one assay. Of the healthcare workers 2.1% (95% CI: 0.8–5.0%) showed IgG nucleocapsid antibodies in at least 2 assays. By contrast, positive controls with proven COVID-19 showed antibody positivity among almost all test systems. Moreover, serum samples obtained from healthcare workers did not show SARS-CoV‑2 neutralizing capacity, in contrast to positive controls. Using a broad spectrum of antibody tests the present study revealed inconsistent results for SARS-CoV‑2 seroprevalence among asymptomatic individuals, while this was not the case among COVID-19 patients. CONEC, ClinicalTrials.gov number NCT04347694read more
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Diagnostic performance of two serological assays for the detection of SARS-CoV-2 specific antibodies: surveillance after vaccination
Alba Fresco-Taboada,Margarita García-Durán,Cristina Aira,Lissette López,Patricia Sastre,L. van der Hoek,Marit J. van Gils,Philip J. M. Brouwer,Rogier W. Sanders,B. Holzer,Irene Zimpernikc,Eduardo López-Collazo,Patricia Muñoz,Paloma Rueda,C. Vela +14 more
TL;DR: In this article , an Enzyme-Linked Immunosorbent Assay (ELISA) and a Lateral Flow Assay were developed based on the SARS-CoV-2 recombinant Receptor Binding Domain (RBD) and the combination of Spike and Nucleoprotein, respectively.
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Vaccination with BNT162b2 and ChAdOx1 nCoV-19 Induces Cross-Reactive Anti-RBD IgG against SARS-CoV-2 Variants including Omicron
Daniel I. Gerges,S. Kapps,Esperanza Hernández-Carralero,Raimundo Freire,Monika Aiad,Sophie Schmidt,Wolfgang Winnicki,Thomas Ritter,Sahra Pajenda,Alice Schmidt,Gere Sunder-Plassmann,Ludwig Wagner +11 more
TL;DR: The low anti-RBD Omicron IgG level could explain the breakthrough infections and their presence could also contribute to a milder COVID-19 course by cross-reactivity and broadening the adaptive immunity.
Journal ArticleDOI
The impact of lockdowns during the COVID-19 pandemic on work-related accidents in Austria in 2020
TL;DR: In this article , the authors investigated the impact of the lockdowns during the COVID-19 (Corona-Virus-Disease 19) pandemic in Austria on work-related accidents in the year 2020.
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Estimating the effects of non-pharmaceutical interventions on COVID-19 in Europe.
Seth Flaxman,Swapnil Mishra,Axel Gandy,H. Juliette T. Unwin,Thomas A. Mellan,Helen Coupland,Charles Whittaker,Harrison Zhu,Tresnia Berah,Jeffrey W. Eaton,Melodie Monod,Azra C. Ghani,Christl A. Donnelly,Christl A. Donnelly,Steven Riley,Michaela A. C. Vollmer,Neil M. Ferguson,Lucy C Okell,Samir Bhatt +18 more
TL;DR: The results show that major non-pharmaceutical interventions and lockdown in particular have had a large effect on reducing transmission and continued intervention should be considered to keep transmission of SARS-CoV-2 under control.
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