CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.
Gianluca Luigi Russo,Giovanna Sonsalla,Poornemaa Natarajan,Christopher T. Breunig,Giorgia Bulli,Juliane Merl-Pham,Sabine Schmitt,Jessica Giehrl-Schwab,Florian Giesert,Martin Jastroch,Hans Zischka,Wolfgang Wurst,Stefan H. Stricker,Stefanie M. Hauck,Giacomo Masserdotti,Magdalena Götz +15 more
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TLDR
The comprehensive mitochondrial proteome of cortical astrocytes and neurons is determined, identifying about 150 significantly enriched mitochondrial proteins for each cell type, including transporters, metabolic enzymes, and cell-type-specific antioxidants.About:
This article is published in Cell Stem Cell.The article was published on 2021-03-04 and is currently open access. It has received 31 citations till now. The article focuses on the topics: Mitochondrion & Proteome.read more
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Transcriptional activation of endogenous Oct4 via the CRISPR/dCas9 activator ameliorates Hutchinson‐Gilford progeria syndrome in mice
TL;DR: In this paper , the authors found that transcriptional activation of the endogenous Oct4 gene by using the CRISPR/dCas9 activator system can efficiently ameliorate hallmarks of aging in a mouse model of HutchinsonGilford progeria syndrome (HGPS).
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Dead Cas(t) light on new life: CRISPRa-mediated reprogramming of somatic cells into neurons
TL;DR: The latest progress, new insights, and future challenges of the application of the dCas9 system in direct neuronal reprogramming in vivo and in vitro are discussed.
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Induced Neurons for Disease Modeling and Repair: A Focus on Non-fibroblastic Cell Sources in Direct Reprogramming.
TL;DR: In this paper, a review highlights studies that utilize non-fibroblastic cell sources for reprogramming, such as astrocytes, olfactory ensheathing cells, peripheral blood cells, Muller glia, and more.
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Non-codon Optimized PiggyBac Transposase Induces Developmental Brain Aberrations: A Call for in vivo Analysis.
TL;DR: In this paper, the piggyBac transposon system has the highest insert size, a seamless integration of the transposed sequence into the host genome, and can be delivered by transfection avoiding viral vectors causing an immune response.
References
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Mutations in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis
TL;DR: Tight genetic linkage between FALS and a gene that encodes a cytosolic, Cu/Zn-binding superoxide dismutase (SOD1), a homodimeric metalloenzyme that catalyzes the dismutation of the toxic superoxide anion O–2 to O2 and H2O2 is reported.
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Universal sample preparation method for proteome analysis
TL;DR: A method is described, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics and allows single-run analyses of organelles and an unprecedented depth of proteome coverage.
Journal ArticleDOI
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Omar O. Abudayyeh,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang +11 more
TL;DR: Structural-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci is described and the potential of Cas9-based activators as a powerful genetic perturbation technology is demonstrated.
Journal ArticleDOI
A Mitochondrial Protein Compendium Elucidates Complex I Disease Biology
David J. Pagliarini,Sarah E. Calvo,Betty Chang,Sunil A Sheth,Scott Bradley Vafai,Shao En Ong,Geoffrey A. Walford,Canny Sugiana,Avihu Boneh,Avihu Boneh,William K. Chen,David E. Hill,Marc Vidal,James G. Evans,David R. Thorburn,David R. Thorburn,Steven A. Carr,Vamsi K. Mootha,Vamsi K. Mootha +18 more
TL;DR: This work predicts 19 proteins to be important for the function of complex I (CI) of the electron transport chain and validate a subset of these predictions using RNAi, including C8orf38, which is shown to have an inherited mutation in a lethal, infantile CI deficiency.
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