Journal ArticleDOI
Derivation of pluripotent epiblast stem cells from mammalian embryos
I. Gabrielle M. Brons,Lucy E. Smithers,Matthew Trotter,Peter J. Rugg-Gunn,Bowen Sun,Susana M. Chuva de Sousa Lopes,Sarah K. Howlett,Amanda Clarkson,Lars Ährlund-Richter,Roger A. Pedersen,Ludovic Vallier +10 more
Reads0
Chats0
TLDR
It is shown that pluripotent stem cells can be derived from the late epiblast layer of post-implantation mouse and rat embryos using chemically defined, activin-containing culture medium that is sufficient for long-term maintenance of human embryonic stem cells.Abstract:
Although the first mouse embryonic stem (ES) cell lines were derived 25 years ago using feeder-layer-based blastocyst cultures, subsequent efforts to extend the approach to other mammals, including both laboratory and domestic species, have been relatively unsuccessful. The most notable exceptions were the derivation of non-human primate ES cell lines followed shortly thereafter by their derivation of human ES cells. Despite the apparent common origin and the similar pluripotency of mouse and human embryonic stem cells, recent studies have revealed that they use different signalling pathways to maintain their pluripotent status. Mouse ES cells depend on leukaemia inhibitory factor and bone morphogenetic protein, whereas their human counterparts rely on activin (INHBA)/nodal (NODAL) and fibroblast growth factor (FGF). Here we show that pluripotent stem cells can be derived from the late epiblast layer of post-implantation mouse and rat embryos using chemically defined, activin-containing culture medium that is sufficient for long-term maintenance of human embryonic stem cells. Our results demonstrate that activin/Nodal signalling has an evolutionarily conserved role in the derivation and the maintenance of pluripotency in these novel stem cells. Epiblast stem cells provide a valuable experimental system for determining whether distinctions between mouse and human embryonic stem cells reflect species differences or diverse temporal origins.read more
Citations
More filters
Journal ArticleDOI
An alternative pluripotent state confers interspecies chimaeric competency
Jun Wu,Daiji Okamura,Mo Li,Keiichiro Suzuki,Chongyuan Luo,Li Ma,Yupeng He,Zhongwei Li,Christopher Benner,Isao Tamura,Marie N. Krause,Joseph R. Nery,Tingting Du,Zhuzhu Zhang,Tomoaki Hishida,Yuta Takahashi,Emi Aizawa,Na Young Kim,Jeronimo Lajara,Pedro Guillen,Josep M. Campistol,Concepcion Rodriguez Esteban,Pablo J. Ross,Alan Saghatelian,Bing Ren,Joseph R. Ecker,Juan Carlos Izpisua Belmonte +26 more
TL;DR: In this article, a region-selective pluripotent stem cells (rsPSCs) were obtained from mouse embryos and primate pluripotency, which can be used to generate post-implantation chimaeric embryos.
Journal ArticleDOI
Competitive Interactions Eliminate Unfit Embryonic Stem Cells at the Onset of Differentiation
Margarida Sancho,Aida Di-Gregorio,Nancy George,Sara Pozzi,Juan Miguel Sanchez,Barbara Pernaute,Tristan A. Rodriguez +6 more
TL;DR: It is found that ESCs displaying defective bone morphogenetic protein signaling or defective autophagy or that are tetraploid are eliminated at the onset of differentiation by wild-type cells in an apoptosis-dependent manner and is mediated by secreted factors.
Journal ArticleDOI
Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo
TL;DR: In this article, the genomewide location of active and repressive histone modifications in embryonic stem cells, trophoblast stem cells and extraembryonic endoderm stem cells from the mouse were compared.
Journal ArticleDOI
New Insights into Early Human Development: Lessons for Stem Cell Derivation and Differentiation
Janet Rossant,Patrick P.L. Tam +1 more
TL;DR: Pathways underlying mouse embryonic development have always informed efforts to derive, maintain, and drive differentiation of human pluripotent stem cells, but direct application to the human system has not always been successful because of fundamental developmental differences between species.
Journal ArticleDOI
Albumin-associated lipids regulate human embryonic stem cell self-renewal.
TL;DR: The discovery of the role played by albumin-associated lipids in stimulating hESC self-renewal constitutes a significant advance in the knowledge of how h ESC pluripotency is maintained by extracellular factors and has important applications in the development of increasingly chemically defined hESCs culture systems.
References
More filters
Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
Establishment in culture of pluripotential cells from mouse embryos
TL;DR: The establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts are reported, able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo.
Journal ArticleDOI
Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
TL;DR: In this article, the authors described the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice and demonstrated the pluripotency of these embryonic stem cells by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types.
Journal ArticleDOI
Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
Journal ArticleDOI
Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.
TL;DR: A role is established for Oct-3/4 as a master regulator of pluripotency that controls lineage commitment and the sophistication of critical transcriptional regulators is illustrated and the consequent importance of quantitative analyses are illustrated.