Journal ArticleDOI
Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture.
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It is shown that addition of bFGF to cultures in the presence of membrane-associated SF and LIF enhances the growth of PGCs and allows their continued proliferation beyond the time when they normally stop dividing in vivo.About:
This article is published in Cell.The article was published on 1992-09-04. It has received 1299 citations till now. The article focuses on the topics: Embryonic Germ Cells & Embryoid body.read more
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Functional expression cloning of nanog, a pluripotency sustaining factor in embryonic stem cells
Ian Chambers,Douglas Colby,Morag Robertson,Jennifer Nichols,Sonia Lee,Susan Tweedie,Austin Smith +6 more
TL;DR: These findings establish a central role for Nanog in the transcription factor hierarchy that defines ES cell identity and confirm that Cytokine dependence, multilineage differentiation, and embryo colonization capacity are fully restored upon transgene excision.
Journal ArticleDOI
Derivation of pluripotent epiblast stem cells from mammalian embryos
I. Gabrielle M. Brons,Lucy E. Smithers,Matthew Trotter,Peter J. Rugg-Gunn,Bowen Sun,Susana M. Chuva de Sousa Lopes,Sarah K. Howlett,Amanda Clarkson,Lars Ährlund-Richter,Roger A. Pedersen,Ludovic Vallier +10 more
TL;DR: It is shown that pluripotent stem cells can be derived from the late epiblast layer of post-implantation mouse and rat embryos using chemically defined, activin-containing culture medium that is sufficient for long-term maintenance of human embryonic stem cells.
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Out of Eden: Stem Cells and Their Niches
Fiona M. Watt,Brigid L.M. Hogan +1 more
TL;DR: Both intrinsic and extrinsic signals regulate stem cell fate and some of these signals have now been identified and can be exploited in the application of stem cells to tissue replacement therapy.
Journal ArticleDOI
Naive and primed pluripotent states.
TL;DR: It is proposed that two phases of pluripotency can be defined: naive and primed, and this distinction extends to pluripotent stem cells derived from embryos or by molecular reprogramming ex vivo.
Journal ArticleDOI
Derivation of pluripotent stem cells from cultured human primordial germ cells
Michael J. Shamblott,Joyce Axelman,Shunping Wang,Elizabeth M. Bugg,John W. Littlefield,Peter J. Donovan,Paul D. Blumenthal,George R. Huggins,John D. Gearhart +8 more
TL;DR: In this article, a set of primordial germ cells (PGCs, 5-9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor (HILI) and forskolin.
References
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Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).
Davor Solter,Barbara B. Knowles +1 more
TL;DR: A monoclonal antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with F9 teratocarcinoma cells is described, which defines an embryonic stage-specific antigen.
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Primordial germ cells in the mouse embryo during gastrulation.
TL;DR: It is argued that the cells in the cluster are indeed primordial germ cells, at a stage significantly earlier than any reported previously, and this would indicate that the PGC lineage in the mouse is set aside at least as early as 7 dpc, and that its differentiation, as expressed by ALP activity, is gradual.
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Platelet-derived growth factor promotes division and motility and inhibits premature differentiation of the oligodendrocyte/type-2 astrocyte progenitor cell.
TL;DR: It is found that platelet-derived growth factor mimics the effects of type-1 astrocytes on O-2A progenitor cells, and antibodies to PDGF block the effects.
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Platelet-derived growth factor from astrocytes drives the clock that times oligodendrocyte development in culture.
TL;DR: It is shown that platelet-derived growth factor (PDGF) can replace type-1-astrocyte-conditioned medium in restoring the normal timing of oligodendrocytes differentiation in vitro and that anti-PDGF antibodies inhibit this property of the appropriately conditioned medium.
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Cooperation between two growth factors promotes extended self-renewal and inhibits differentiation of oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells.
TL;DR: This work has found that O-2A progenitors can be induced to undergo continuous self-renewal in the absence of oligodendrocytic differentiation by exposure to a combination of PDGF and basic fibroblast growth factor (bFGF).